Topic
Substrate (chemistry)
About: Substrate (chemistry) is a research topic. Over the lifetime, 35902 publications have been published within this topic receiving 740722 citations. The topic is also known as: enzyme substrate.
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TL;DR: The conversion of [13l-3H, 3-14C]8,11,14-eicosatrienoic acid is accompanied by an isotope effect, suggesting that the hydrogen removal occurs as the initial step of the reaction, and only the hydrogen of the l configuration is removed by the enzyme during the conversion.
633 citations
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TL;DR: There are a number of methods that can be used for the preparation of enzyme-containing lipid vesicles (liposomes) which are lipid dispersions that contain water-soluble enzymes in the trapped aqueous space, and a review of these studies is given and some of the main results are summarized.
619 citations
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TL;DR: A new substrate for chymotrypsin, succinyl-Ala—Ala —Pro—Phe-p-nitroanilide has been synthesized and is readily soluble and stable to hydrolysis in Tris buffer at pH 8.0.
600 citations
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01 Jan 1974
TL;DR: This chapter discusses about lactate dehydrogenase (LDH), which was first crystallized from rat muscle in 1943, which is applied in biochemistry and clinical chemistry and is not linked to the pyridine nucleotide coenzymes.
Abstract: Publisher Summary
This chapter discusses about lactate dehydrogenase (LDH), which was first crystallized from rat muscle in 1943. LDHs from bacteria and yeast are not linked to the pyridine nucleotide coenzymes. The LDH activity in serum results from the presence of different enzyme proteins with the same action and substrate specificity, but of different origin. LDH is applied in biochemistry and clinical chemistry. The optimum conditions for the measurements of the enzyme in serum after myocardial infarction, in liver damage, blood diseases, and tumors have been more or less established. The sodium pyruvate content should be at least 99%. Deterioration of the buffer/substrate solution is usually because of bacterial contamination, which can be prevented by addition of a few drops of chloroform. Sudhof et al. have measured the loss of activity on storage of serum at various temperatures. With highly active serum a large proportion of the NADH can be oxidized between the addition of the serum and the first reading. LDH is a cytoplasmic enzyme; simple homogenization in a Potter–Elvehjem homogenizer is sufficient to completely extract the entire enzyme.
598 citations
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TL;DR: A protein kinase which phosphorylated histone and protamine was partially purified from bovine cerebellum and suggested that the enzyme was produced from its precursor protein by a limited proteolytic reaction.
586 citations