Topic
Substrate (chemistry)
About: Substrate (chemistry) is a research topic. Over the lifetime, 35902 publications have been published within this topic receiving 740722 citations. The topic is also known as: enzyme substrate.
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TL;DR: A thermophilic bacterium isolated from a hot spring area of Yellowstone National Park was isolated and its enzyme activity was stable to both hydrogen peroxide and alkaline protease which are detergent ingredients.
185 citations
TL;DR: The efficient protection against thiol reagent inhibition by substrate supports a supposition that this -SH group forms a covalent bond with substrate during the catalytic reaction.
185 citations
Patent•
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30 Oct 1986
TL;DR: In this paper, an enzyme-labeled binding assay is performed using an elongate strip provided with transverse reagent zones, where the substrate moves slower through the absorbent material than the enzyme-labelled reagent or any compound of the enzyme formed in the assay.
Abstract: A device for performing an enzyme-labelled binding assay comprises an absorbent material and a developing solution, wherein the absorbent material is provided with a plurality of reagent zones including an indicator reagent zone, and is capable of transporting the developing solution by capillary action sequentially through each reagent zone, and wherein the indicator reagent zone includes a reagent capable, directly or indirectly, of immobilising an enzyme-labelled reagent in an amount dependent upon the assay result, characterised in that the developing solution includes a signal producing substrate for the enzyme. The substrate moves slower through the absorbent material than the enzyme-labelled reagent or any compound of the enzyme-labelled reagent formed in the assay. The absorbent material is suitably in the form of an elongate strip provided with transverse reagent zones. The device is useful for performing immunoassays including immunometric assays and dual analyte assays.
185 citations
TL;DR: In this paper, a novel method consisting of two dry process techniques, that is, nanotexturing by an oxygen plasma treatment and subsequent hydrophobic coating by means of low temperature chemical vapor deposition or plasma-enhanced chemical vapor deblurring, was employed to form ultra water-repellent polymer sheets.
185 citations
TL;DR: A rapid, simple, and accurate method for the chemical assay of angiotensin-converting enzyme has been developed that relies on the use of 2,4,6-trichloro-s-triazine (TT) as a colorimetric reagent of hippuric acid (N-benzoylglycine).
185 citations