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Surface plasmon resonance

About: Surface plasmon resonance is a research topic. Over the lifetime, 24909 publications have been published within this topic receiving 810976 citations. The topic is also known as: Surface plasmon resonance & SPR (technology).


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Journal ArticleDOI
TL;DR: Results indicate that an alkanethiol SAM based SPR biosensor has the potential for rapid and specific detection of E. coli O157:H7, using a sandwich assay.

280 citations

Journal ArticleDOI
TL;DR: A new method for constructing oligonucleotide arrays on gold surfaces has been developed, and these arrays have been used in DNA hybridization experiments with in situ surface plasmon resonance (SPR) imaging detection, able to differentiate between single- and double-stranded DNA regions on the gold surface.
Abstract: A new method for constructing oligonucleotide arrays on gold surfaces has been developed, and these arrays have been used in DNA hybridization experiments with in situ surface plasmon resonance (SPR) imaging detection. The detection technique was able to differentiate between single- and double-stranded DNA regions on the gold surface. The hybridization of both oligonucleotides and PCR-amplified DNA fragments was detectable, with the latter exhibiting slower hybridization kinetics. Temperature control of the in situ SPR cell was used to discriminate between perfectly matched duplexes and single-base-mismatched duplexes. The SPR detection technique requires no label on the DNA, but fluorescently labeled targets were also tested and detected by fluorescence imaging as an independent verification of the hybridization behavior of these DNA arrays. The in situ SPR imaging method for detection of DNA hybridization is expected to complement other existing methods for study of DNA interactions and might find futu...

280 citations

Journal ArticleDOI
TL;DR: In this paper, an algorithm for calculating the electromagnetic field distribution in multilayer structure is developed relying on Abeles matrices method for wave propagation in isotropic stratified media.
Abstract: The performance of surface plasmon resonance (SPR) sensors depends on the design parameters. An algorithm for calculating the electromagnetic fields distribution in multilayer structure is developed relying on Abeles matrices method for wave propagation in isotropic stratified media. The correlation between field enhancement and sensitivity enhancement is examined and found to agree with the overlap integral in the analyte region. This correlation was verified in the conventional SPR sensor based on Kretschmann configuration, and in the improved SPR sensor with high refractive index dielectric top layer for several cases, e.g. field enhancement due to resonance, the sensitivity dependence on the wavelength, the influence of prism refractive index on sensitivity, and the effect of the layers materials and thicknesses.

280 citations

Journal ArticleDOI
TL;DR: Binding of streptavidin at 1 nM concentration induces a mean resonant wavelength shift of 0.59 nm suggesting that the current optical setup is able to reliably measure wavelength shifts as small as 0.3 nm, which is close to the limit of detection of the system.
Abstract: We report the use of individual gold nanorods as plasmonic transducers to detect the binding of streptavidin to individual biotin-conjugated nanorods in real time on a surface Label-free detection at the single-nanorod level was performed by tracking the wavelength shift of the nanorod-localized surface plasmon resonant scattering spectrum using a dark-field microspectroscopy system The lowest streptavidin concentration that was experimentally measured was 1 nM, which is a factor of 1000-fold lower than the previously reported detection limit for streptavidin binding by biotinylated single plasmonic nanostructures We believe that the current optical setup is able to reliably measure wavelength shifts as small as 03 nm Binding of streptavidin at 1 nM concentration induces a mean resonant wavelength shift of 059 nm suggesting that we are currently operating at close to the limit of detection of the system

280 citations

Journal ArticleDOI
TL;DR: Observations support the recent hypothesis that the extent of metal-enhanced fluorescence is due to the ability of surface plasmons to radiate coupled fluorophore fluorescence and correlate increases in fluorescence emission with an increased particle size, providing strong experiment evidence for this hypothesis.
Abstract: The effects of thermally annealed silver island films have been studied with regard to their potential applicability in applications of metal-enhanced fluorescence, an emerging tool in nano-biotechnology. Silver island films were thermally annealed between 75 and 250°C for several hours. As a function of both time and annealing temperature, the surface plasmon band at ≈420 nm both diminished and was blue shifted. These changes in plasmon resonance have been characterized using both absorption measurements, as well as topographically using Atomic Force Microscopy. Subsequently, the net changes in plasmon absorption are interpreted as the silver island films becoming spherical and growing in height, as well as an increased spacing between the particles. Interestingly, when the annealed surfaces are coated with a fluorescein-labeled protein, significant enhancements in fluorescence are osbserved, scaling with annealing temperature and time. These observations strongly support our recent hypothesis that the extent of metal-enhanced fluorescence is due to the ability of surface plasmons to radiate coupled fluorophore fluorescence. Given that the extinction spectrum of the silvered films is comprised of both an absorption and scattering component, and that these components are proportional to the diameter cubed and to the sixth power, respectively, then larger structures are expected to have a greater scattering contribution to their extinction spectrum and, therefore, more efficiently radiate coupled fluorophore emission. Subsequently, we have been able to correlate our increases in fluorescence emission with an increased particle size, providing strong experiment evidence for our recently reported metal-enhanced fluorescence, facilitated by radiating plasmons hypothesis.

280 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20231,320
20222,684
20211,240
20201,422
20191,498
20181,528