Showing papers on "Tartrate-resistant acid phosphatase published in 1981"
TL;DR: Tartrate-resistant acid phosphatase was isolated from serum and spleen of patients affected by Gaucher's disease and electrophoretic and antigenic properties were compared to the enzyme isolated from hairy cells described in a previous study.
49 citations
TL;DR: The data suggest that mouse tissue is a good animal model to study the normal cell type with strong tartrate‐resistant acid phosphatase, and enzyme histochemical studies showed many nonphagocytic cells with strong Tartrate‐ resistant acidosphatase in spleen.
Abstract: A strong activity of the tartrate-resistant acid phosphatase, isoenzyme 5b, was observed in mouse spleen. The enzyme of the mouse spleen had similar electrophoretic mobility and related antigenicity to that of the reticulum cells of leukemic reticuloendotheliosis. Enzyme histochemical studies showed many non-phagocytic cells with strong tartrate-resistant acid phosphatase in spleen. The data suggest that mouse tissue is a good animal model to study the normal cell type with strong tartrate-resistant acid phosphatase.
12 citations
2 citations
TL;DR: It is found that chloride ions increase the trapping efficiency of the medium without decreasing the turnover number of the particular acid phosphatase under study, a more likely explanation for the favourable properties of the chloride-enriched medium.
Abstract: The Gomori method has been notorious for its irreproducibility and inefficiency. It is to be welcomed, therefore, that in a recent paper Waters & Butcher (1980) analyse a number of Gomori media for their actual lead content and describe the conditions for the preparation of a stable medium. Their article ends with the remark that ' . . . the use of such media should allow more detailed studies to be made of the effects of the composition of the incubation medium on the reaction in the section'. It is regrettable, therefore, that they do not incorporate in their discussion some recent findings in which, with the aid of a new polyacrylamide model system, the influence of some variations in media composition was demonstrated objectively (De Jong et al., 1978, 1979a, b). From these investigations, the essential role of chloride ions for increasing the trapping efficiency of the medium without changing its turnover number has emerged. A Gomori-chloride medium was shown to be superior to the classical Gomori medium in trapping efficiency in the model. The reason for this favourable effect is probably due to the incorporation of the chloride ion in the lead phosphate precipitate with concomitant change in its properties. The model was also found useful for studying other precipitation processes (De Jong et al., 1980). At the end of their paper, Waters & Butcher seem to give the impression that we recommended the addition of chloride ions on the same theoretical basis as did Reed & Wenzel (1975), who thought its effect to be due to the influence on the osmolality of the medium. We have found, however, that chloride ions increase the trapping efficiency of the medium without decreasing the turnover number of the particular acid phosphatase under study. This seems to us a more likely explanation for the favourable properties of the chloride-enriched medium. In some investigations, when adjusting the pH of the buffer-substrate solution accidently with HC1 or a different acid, some workers might have obtained different results without realising its basis. We have also found that the lead ion-containing solution should not, even temporarily during a mixing procedure, have a pH higher than 7. On this point we agree with Waters & Butcher.