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Taspine

About: Taspine is a research topic. Over the lifetime, 73 publications have been published within this topic receiving 1377 citations.


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Journal ArticleDOI
TL;DR: Results indicated that taspine, with antitumor activity, could interact with the cell, act on EGFR at the cell membrane, and inhibit cell proliferation by down-regulating EGFR protein and mRNA expression.
Abstract: Taspine was isolated for the first time from Radix et Rhizoma Leonticis. Epidermal growth factor receptor (EGFR) is important in cell growth and differentiation and has become an important target in anti-cancer drug design. In this study, we found taspine could inhibit proliferation of A431 and HEK293/EGFR cells. To investigate whether it could enter the cell or acted on the cell membrane receptor, a cell-based assay was established to determine the concentration of taspine in A431 and HEK293/EGFR cells using high-performance liquid chromatography–mass spectrometry (HPLC–MS). The inhibitory effects of taspine on EGFR expression and mRNA expression were also investigated. The HPLC–MS results showed that taspine decreased in the supernatant liquid, and increased in the cell lysate, which indicated that taspine could enter the cell or act on the cell membrane receptor. Subsequent analysis using a cell-membrane chromatography (CMC) assay showed that taspine could act on EGFR at the cell membrane. In addition, ELISA and reverse transcriptase polymerase chain reaction (RT-PCR) assays showed that taspine inhibited proliferation, EGFR protein, and EGFR mRNA expression in A431 and HEK293/EGFR cells. These results indicated that taspine, with antitumor activity, could interact with the cell, act on EGFR at the cell membrane, and inhibit cell proliferation by down-regulating EGFR protein and mRNA expression.

6 citations

Journal Article
TL;DR: Taspine enhances wound healing by increasing the autocrine of TGF-beta1 and EGF by fibroblast by accelerating the recovery of skin wound.
Abstract: Objective To study the effect of taspine on enhancement of skin wound healing and its effect on fibroblast proliferation and autocrine. Methods The plerosis effect of taspine on experimental skin wound was observed in vivo. Different concentrations of taspine were added in vitro and MTT technique was applied to observe its effect on fibroblast proliferation, the levels of transforming growth factor-beta1 (TGF-13P) and epidermal growth factor (EGF) were determined by ELISA. Results In vivo, exo-applied taspine 300 microg and 150 microg accelerated the recovery of skin wound. In vitro, 0.50-0.4 microg/ml taspine could increase autocrine of TGF-beta1and EGF by fibroblast, but it showed no effect on L929 fibroblast proliferation. Conclusions Taspine enhances wound healing by increasing the autocrine of TGF-beta1 and EGF by fibroblast.

6 citations

Journal ArticleDOI
TL;DR: In this paper, the total synthesis of taspine, starting from ferulic acid and isovanillin, was reported, and the successful synthetic strategy was then applied to the synthesis of a symmetrical analogue TAS2C.

6 citations

Journal ArticleDOI
Bingling Dai1, Wenjie Wang1, Yujiao Ma1, Rui Liu1, Yanmin Zhang1 
TL;DR: The findings showed that 12k had an inhibitory effect on the growth of Caco-2 cells, and it functioned by interrupting the phosphorylation of EphrinB2 and its related signaling pathway.
Abstract: Colorectal cancer is a common gastrointestinal malignancy worldwide and it is a lethal and aggressive malignancy with a dismal prognosis. In the present study, we investigated the effect of taspine derivative 12k on human colorectal cancer targeted at EphrinB2 and its PDZ. The results indicated that 12k could bind to EphrinB2 and showed a higher suppressive effect on EphrinB2/HEK293 than on HEK293 cells. Caco‑2 cells were screened for high expression of EphrinB2. We found that 12k not only significantly decreased Caco‑2 cell viability and colony formation but impaired migration. Meanwhile, 12k effectively inhibited blood vessel formation in a tissue model of angiogenesis. Mechanistic studies revealed that 12k significantly reduced the phosphorylation of EphrinB2 and PDZ protein PICK1. Accordingly, it was associated with the downregulation by 12k of the PI3K/AKT/mTOR and MAPK signaling pathways which were downstream of VEGFR2, yet it had no effect on VEGFR3. Moreover, the expression of CD34, CD45 and HIF‑1α were downregulated in the Caco‑2 cells. In conclusion, our findings showed that 12k had an inhibitory effect on the growth of Caco-2 cells, and it functioned by interrupting the phosphorylation of EphrinB2 and its related signaling pathway.

5 citations

Journal ArticleDOI
TL;DR: In this paper, a nonaqueous capillary electrophoresis (NACE) method has been developed for the simultaneous determination of magnoflorine, taspine, and caulophine in Caulophyllum robustum collected in different seasons.
Abstract: A non-aqueous capillary electrophoresis (NACE) method has been developed for the simultaneous determination of magnoflorine, taspine, and caulophine in Caulophyllum robustum collected in different seasons. The relative standard deviations (RSDs) of the migration times and peak areas of the three components were 0.33 and 3.34% for magnoflorine, 0.45 and 2.67% for taspine, and 0.49 and 3.71% for caulophine, respectively. Detection limits of magnoflorine, taspine, and caulophine were 0.30 μg/mL, 0.08 μg/mL, and 0.10 μg/mL, respectively. This method has been successfully applied to simultaneous determination of the three bioactive components in the different collection seasons.

5 citations

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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20212
20193
20161
20152
20143
20134