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Showing papers on "TEC published in 2001"


Journal ArticleDOI
TL;DR: In this article, the authors examined the ionospheric total electron content (TEC) served by a network of the global positioning system (GPS) receivers in Taiwan area.
Abstract: On 20 September1999 UT (21 Septemberin local time, LT) a large earthquake Mw=7.7 struck central Taiwan nearthe small town of Chi-Chi. The greatest plasma frequency in the ionosphere, foF2, observed by the Chung- Li ionosonde (25.0 ◦ N, 121.2 ◦ E) reveals three clear precur- sors at 1, 3, and 4 days prior to the earthquake. This paper examines the ionospheric total electron content (TEC) ob- served by a network of the global positioning system (GPS) receivers in Taiwan area. It is found that variations in foF2 and overhead TEC recorded at Chung-Li have a similar ten- dency. Combining the data of the network of 13 GPS re- ceivers, time, and spatial variations of TEC prior to the Chi- Chi earthquake are examined. Results show that the equa- torial anomaly crest moves equatorward and its TEC value significantly decreases 1, 3, and 4 days before the earth- quake. A comparison between the disturbed and reference (previous 15-day median) days confirms that TEC decreases significantly around the epicenter in the afternoons of these days. Finally, possible mechanisms are proposed and dis- cussed.

310 citations


Journal ArticleDOI
TL;DR: The purpose of this essay is to characterize members of the Tec family and discuss their involvement in signaling, and the three‐dimensional structure, expression pattern and evolutionary aspects will also be considered.
Abstract: Cytoplasmic protein-tyrosine kinases (PTKs) are enzymes involved in transducing a vast number of signals in metazoans, The importance of the Tec family of kinases was immediately recognized when, i ...

309 citations


Journal ArticleDOI
TL;DR: In this article, the ionospheric effects of a halo coronal mass ejection (CME) initiated on the Sun on September 20, 1999, and causing the largest magnetic storm during this month on September 22, 23, and 24, 1999 were studied through their effects on a prototype of a Global Positioning System (GPS)-based navigation system called Wide Area Augmentation System (WAAS) and their impact on global VHF/UHF communication systems.
Abstract: In this paper we present a study of the ionospheric effects of a halo coronal mass ejection (CME) initiated on the Sun on September 20, 1999, and causing the largest magnetic storm during this month on September 22–23, 1999, with the hourly Dst index being −167 nT at ∼2400 UT on September 22. The recurrent CME on October 18 caused an even larger magnetic storm on October 22, 1999, with Dst of −231 nT at ∼0700 UT. The ionospheric effects of these two major magnetic storms are studied through their effects on a prototype of a Global Positioning System (GPS)-based navigation system called Wide Area Augmentation System (WAAS) being developed by the Federal Aviation Administration for use in the continental United States and their impact on global VHF/UHF communication systems. It is shown that the penetration of transient magnetospheric electric fields equatorward of the shielding region at midlatitudes, which have been well-correlated in the past with rapid changes in the well-known Dst index (or through its recently available high resolution 1-min counterpart the SYM-H index), can cause large increases of total electron content (TEC), TEC fluctuations, and saturated 250-MHz scintillation, and these, in turn, may have significant impacts on WAAS. The local time of Dst changes (and not just Dst magnitude) was found to be very important for WAAS, since the largest effects on TEC are seen near dusk. The prompt penetration of these magnetospheric electric fields all the way to the magnetic equator causes augmentation or inhibition of equatorial spread F. The global ionospheric response to these storms has been obtained from ground-based TEC observations with a GPS network and space-based in situ density and electric field measurements using the Republic of China Satellite-1 (ROCSAT-I) and several Defense Meteorological Satellite Program satellites. These prompt penetration electric fields cause VHF/UHF scintillations and GPS TEC variations at low latitudes in the specific longitude sector for which the early evening period corresponds to the time of rapid Dst variations and maximum Dst phase. The effects of the delayed ionospheric disturbance dynamo and those of decreased magnetospheric convection on postmidnight irregularity generation are shown to be confined to a part of the same longitude range that actively responded to the prompt penetration of electric fields in the early evening sector.

235 citations



Journal ArticleDOI
TL;DR: The protocols for transformation by spheroplasting, LiAc/ssDNA/PEG, and electroporation are compared, and possible mechanisms for transformation are discussed.
Abstract: Genetic transformation was first described by Griffith in 1928 and has since been demonstrated in a variety of organisms, including many species of fungi. This review focuses on the history and tec...

207 citations


Journal ArticleDOI
TL;DR: This work addresses the widespread concern that TEC is too unspecified and, therefore, too vague in a number of important respects, and elaborate on its views about TEC's relations to other important frameworks and approaches in the field.
Abstract: First, we discuss issues raised with respect to the Theory of Event Coding (TEC)'s scope, that is, its limitations and possible extensions Then, we address the issue of specificity, that is, the widespread concern that TEC is too unspecified and, therefore, too vague in a number of important respects Finally, we elaborate on our views about TEC's relations to other important frameworks and approaches in the field like stages models, ecological approaches, and the two-visual-pathways model

197 citations


Journal ArticleDOI
TL;DR: In this article, a new technique for calculating the vertical total electron content (TEC) from ground-based ionosonde measurements is introduced, where the ionogram provides the information to directly calculate the vertical electron density profile up to the peak of the F2 layer.
Abstract: A new technique for calculating the vertical total electron content (TEC) from ground-based ionosonde measurements is introduced. The ionogram provides the information to directly calculate the vertical electron density profile up to the peak of the F2 layer. The profile above the peak is approximated by an α-Chapman function with a scale height that is derived from the profile shape around the F2 peak. The ionosonde TEC, or ITEC, is then calculated as the integral from 0 to ∞ over the entire profile. ITEC values from Digisonde observations at Millstone Hill, Wallops Island, and Jicamarca are compared with incoherent scatter radar and with Faraday and TOPEX satellite TEC measurements, showing very good agreement at middle latitudes and the magnetic equator.

193 citations


Journal ArticleDOI
TL;DR: In this paper, a new technique of estimating the ionospheric topside profile from the information contained in the ground-based ionograms is introduced, where the electron density profile above the F2 layer peak is approximated by an α-Chapman function with a constant scale height that is derived from the bottomside profile shape near the F 2 peak.

169 citations


Patent
10 Oct 2001
TL;DR: In this article, a self-contained thermoelectric cooler (TEC) is provided by utilizing a DC-to-DC active power supply to provide compact size and a non-planar barrier between the hot side and cold side of the TEC.
Abstract: A compact self-contained thermoelectric cooler (TEC) is provided by utilizing a DC to DC active power supply to provide compact size. The compactness and flatness of the DC to DC active power supply allows the unit to be completely self-contained. The compactness and flatness of the DC to DC active power supply allow the power supply assembly to be located on the hot side of the TEC. A non-planar barrier between the hot side and cold side of the TEC also provides compactness and allows the TEC to be completely self-contained. A mounting frame is disposed between the hot and cold side. The mounting frame includes a power pack cutout allowing a non-planar barrier between the hot and cold side. Electrical components of the power supply are mounted to a power pack heat sink. The power pack heat sink is attached to the mounting frame with electrical components protruding through the power pack cutout. Power pack cover is attached to the cold side of the mounting frame, encapsulating the electrical components and creating a non-planar barrier between the hot side and the cold side of the TEC. The TEC includes several moisture resistant barriers and thermally isolating barriers to improve performance and longevity. A moisture resistant barrier is provided around at least one thermoelectric module and around a power supply assembly. A non-planar thermally isolating barrier is provided between the hot side and cold side of the TEC. A power control system is provided to deliver maximum cooling power for a given design and a give set of operating conditions. Finally, a dual power supply may be provided to increase reliability.

118 citations


Journal ArticleDOI
TL;DR: In this article, the ionospheric total electron contents (TEC) in both northern and southern equatorial anomaly regions were examined by using the Global Positioning System (GPS) in Asian area.
Abstract: The ionospheric total electron contents (TEC) in both northern and southern equatorial anomaly regions are examined by using the Global Positioning System (GPS) in Asian area. The TEC contour charts obtained at YMSM (25.2°N, 121.6°E; 14.0°N geomagnetic) and DGAR (7.3°S, 72.4°E; 16.2°S geomagnetic) stations in 1997, solar minimum, are investigated. It is found that the ionospheric crests manifest remarkable seasonal variations. The TEC values on both northern and southern equatorial anomaly crests yield their maximum values during the vernal and autumnal months, but the winter anomaly does not appear in the southern region. Results show that both crests are fully developed around midday in winter, postnoon in equinoxes and late afternoon in summer, and the two crests move significantly equatorward in winter but slightly poleward in summer and autumn. These phenomena can be fully explained by a combined theory of the transequatorial netural wind, the subsolar point, and the auroral equatorward wind.

85 citations


Journal ArticleDOI
TL;DR: In this paper, the authors constructed latitudinal profiles of the total electron content (TEC) using measurements from six GPS receivers conducted during 1998, and divided the TEC profiles into two groups: one corresponds to days when plumes or equatorial spread F (ESF) develops, and the second group portrays days of no-ESF condition, based on the signature of coherent echoes measured by the Jicamarca Unattended Long-Term Investigation (JULIA) radar and records of scintillations from two sites spaced in latitude.
Abstract: We have constructed latitudinal profiles of the total electron content (TEC) using measurements from six GPS receivers conducted during 1998. The TEC profiles have been divided into two groups: One corresponds to days when plumes or equatorial spread F (ESF) develops, and the second group portrays days of no-ESF condition. The presence/absence of ESF is based on the signature of the coherent echoes measured by the Jicamarca Unattended Long-Term Investigation (JULIA) radar and records of scintillations from two sites spaced in latitude. One scintillation station is located near the magnetic equator (Ancon) and the other 12° southward (Antofagasta). The TEC profiles display the typical day-to-day and seasonal variability seen at low latitudes. During the equinoxes, we observed quite often the crests of the anomaly located between 12° and 20° away from the magnetic equator and a trough in-between. The monthly distribution of the appearance of the anomaly and the local time of their appearance are in very good agreement with the reported variability of the upward vertical drifts and the current theory of the equatorial fountain effect. During the equinoxes and the December solstice, the TEC anomaly is observed almost every day, sometimes when there is no ESF activity. Nevertheless, fine inspection of the TEC latitudinal profiles suggests the existence of a close relationship between the temporal evolution of the TEC profiles near sunset and the onset of ESF. We have examined the TEC latitudinal distributions in two different ways. First, we calculated time difference profiles using the distributions corresponding to 1800 and 2000 LT. Second, we used a parameterization of the TEC distributions obtained at 2000 LT. The first method indicates quite drastic increases of the crest values and sharp decreases near the trough during ESF days. In contrast, during days of no ESF there exist almost uniform TEC decreases at all latitudes. The second method displays a preferred high crest/trough ratio (>2), small TEC values at the trough, and large latitudinal integrated values during ESF events.

Journal ArticleDOI
TL;DR: It is demonstrated that Tec plays a role in TCR signaling leading to IL-2 gene induction and TCR-mediated Tec tyrosine phosphorylation, and the Tec kinase PH domain and phosphatidylinositol 3-kinase in Tec signaling downstream of the TCR are implicate.
Abstract: Tec, the prototypical member of the Tec family of tyrosine kinases, is abundantly expressed in T cells and other hemopoietic cell types. Although the functions of Itk and Txk have recently been investigated, little is known about the role of Tec in T cells. Using antisense oligonucleotide treatment to deplete Tec protein from primary T cells, we demonstrate that Tec plays a role in TCR signaling leading to IL-2 gene induction. Interestingly, Tec kinases are the only known family of tyrosine kinases containing a pleckstrin homology (PH) domain. Using several PH domain mutants overexpressed in Jurkat T cells, we show that the Tec PH domain is required for Tec-mediated IL-2 gene induction and TCR-mediated Tec tyrosine phosphorylation. Furthermore, we show that Tec colocalizes with the TCR after TCR cross-linking, and that both the Tec PH and Src homology (SH) 2 domains play a role in this association. Wortmannin, a phosphatidylinositol 3-kinase inhibitor, abolishes Tec-mediated IL-2 gene induction and Tec tyrosine phosphorylation, and partially suppresses Tec colocalization with the activated TCR. Thus, our data implicate the Tec kinase PH domain and phosphatidylinositol 3-kinase in Tec signaling downstream of the TCR.

Journal ArticleDOI
TL;DR: In this article, the authors used the method involving a global spatial averaging of disturbance spectra of the total electron content (TEC) to characterize the TID intensity quantitatively, and suggested that a new global index of the degree of disturbance should be used, which is equal to the mean value of the rms variations in TEC within the selected range of spectral periods.
Abstract: . In this paper an attempt is made to verify the hypothesis of the role of geomagnetic disturbances as a factor in determining the intensity of traveling ionospheric disturbances (TIDs). To improve the statistical validity of the data, we have used the method involving a global spatial averaging of disturbance spectra of the total electron content (TEC). To characterize the TID intensity quantitatively, we suggest that a new global index of the degree of disturbance should be used, which is equal to the mean value of the rms variations in TEC within the selected range of spectral periods (of 20– 60 min, in the present case). The analysis has been made for a set of 100 to 300 GPS stations for 10 days with a different level of geomagnetic activity (Dst from 0 to –350 nT; the Kp index from 3 to 9). It was found that power spectra of daytime TEC variations in the range of 20–60 min periods under quiet conditions have a power-law form with the slope index k = –2.5. With an increase in the level of magnetic disturbance, there is an increase in the total intensity of TIDs, with a concurrent kink of the spectrum caused by an increase in oscillation intensity in the range of 20–60 min. The TEC variation amplitude is found to be smaller at night than during the daytime, and the spectrum decreases in slope, which is indicative of a disproportionate increase in the amplitude of the small-scale part of the spectrum. It was found that an increase in the level of geomagnetic activity is accompanied by an increase in the total intensity of TEC; however, it does not correlate with the absolute level of Dst, but rather with the value of the time derivative of Dst (a maximum correlation coefficient reaches –0.94). The delay of the TID response of the order of 2 hours is consistent with the view that TIDs are generated in auroral regions, and propagate equatorward with the velocity of about 300–400 m/s. Key words. Ionosphere (ionospheric disturbances; auroral ionosphere; equatorial ionopshere)

Journal ArticleDOI
TL;DR: In this article, the authors used the Millstone Hill incoherent scatter radar database to investigate the spatial extent and temporal evolution of TEC and density altitude/latitude structure at middle and subauroral latitudes as a function of solar cycle, local time, and level of geomagnetic activity.
Abstract: The subauroral ionosphere, at the magnetic latitudes which characterize the northeastern United States, is subject to severe F region ionospheric density structuring due to the space weather effects of magnetospheric disturbance electric fields. Communications and navigation systems relying on transionospheric propagation must be able to compensate for the effects of the sharp changes (>10X) in total electron content (TEC) associated with the ionospheric trough and storm time disturbance effects at midlatitudes. The Millstone Hill incoherent scatter radar database has been used to investigate the spatial extent and temporal evolution of TEC and density altitude/latitude structure at middle and subauroral latitudes as a function of solar cycle, local time, and level of geomagnetic activity. More than 11,000 radar elevation scans covering >20° of latitude and altitudes between 150 and 750 km have been used to identify the characteristics of the density gradient near the equatorward edge of the ionospheric trough in a variety of circumstances spanning 20 years and two solar cycles. Pronounced density gradients can be identified in ∼35% of the Millstone Hill scans, and we present a statistical characterization of average magnitude and location for these steepest TEC gradients. In some cases (especially near noon) the equatorward edge of the trough lies poleward of our observational field of view, and gradients associated with phenomena other than the trough contribute to our statistics. On most days the trough appears in the radar scans between 1600 and 2000 magnetic local time (MLT). Larger TEC gradients occur at solar maximum and when the background TEC is higher. The steepest gradients occur in an environment of high TEC (at solar maximum and adjacent to regions of storm-enhanced density (SED)), when the processes which generate the trough are strongest (high Kp). High gradient values occur in the sunlit sector, with maximum values of TEC gradient (∼10 TEC/deg latitude, with 1 TEC unit = 10 16 el m -2 ) found in the postnoon ionosphere. Mean solar maximum TEC gradient at 1600 MLT is 3-4 TEC/deg for Kp 100 over New England and TEC gradients of ∼50 TEC/deg.

Journal ArticleDOI
TL;DR: In this paper, the authors used a low Earth orbiting (LEO) satellite to receive multi-channel Global Positioning System (GPS) carrier phase signals and demonstrate active limb sounding of the Earth's atmosphere and ionosphere by radio occultation technique.
Abstract: The Global Positioning System/Meteorology (GPS/MET) mission has been the first experiment to use a low Earth orbiting (LEO) satellite (the MicroLab-1) to receive multi-channel Global Positioning System (GPS) carrier phase signals and demonstrate active limb sounding of the Earth’s atmosphere and ionosphere by radio occultation technique. Under the assumption of spherical symmetry at the locality of the occultation, the dual-band phase data have been processed to yield ray-path bending angle profiles, which have then been used to yield profiles of refractive index via the Abel integral transform. The refractivity profiles can then, in turn, yield profiles of ionospheric electron density and other atmospheric variables such as neutral atmospheric density, pressure, and temperature in the stratosphere and upper troposphere, and water vapor in the lower troposphere with the aid of independent temperature data. To approach a near real-time process, electron density profiles can also be derived by the Abel transform through the computation of total electron content (TEC) assuming straight-line propagation (neglecting bending). In order to assess the accuracy of the GPS/MET ionospheric electron density retrievals, coincidences of ionosonde data with GPS/MET occultations have been examined. The retrieved electron density profiles from GPS/MET TEC observations have been compared with ionogram inversion results derived from digital ionospheric sounders operated by the National Central University (the Chung-Li digisonde; 24.6°N, 121.0°E) and by Utah State University (the Bear-Lake dynasonde; 41.9°N, 111.4°W). A fuzzy classification method for the automatic identification and scaling of ionogram traces has been applied to recorded ionograms, and then bottomside ionospheric electron density profiles are determined from true-height analysis. The comparison results show better agreement for both of the derived electron density profiles and the F2-layer critical frequency ( foF2) at mid-latitude observations than at low-latitude observations. The rms foF2 differences from the GPS/MET retrievals are 0.61 MHz to the Bear-Lake dynasonde measurements and 1.62 MHz to the Chung-Li digisonde measurements.

Journal ArticleDOI
TL;DR: In this article, an iterative learning control (ILC) technique based on a quadratic optimal criterion is proposed for temperature uniformity control of a wafer under rapid thermal processing (RTP).
Abstract: An iterative learning control (ILC) technique based on a quadratic optimal criterion is proposed for temperature uniformity control of a wafer under rapid thermal processing (RTP). The proposed tec...

13 Jun 2001
TL;DR: The GSV4004 GPS ionospheric scintillation and TEC monitor (GISTM) as discussed by the authors is a dual-frequency GPS signal monitor with a low phase noise (LPSN) oscillator.
Abstract: GPS signals provide an excellent means for measuring ionospheric scintillation effects on a global basis because the signals are continuously available and can be measured through many points of the ionosphere simultaneously. GPS signals are themselves affected. However, tracking through disturbances with a GPS receiver is usually possible with reasonably wide bandwidth tracking loops. Because of this, ionospheric scintillation can be monitored, and is currently being monitored around the world. This was not widely possible during the last solar activity peak. The importance of the wide bandwidth is that scintillation parameters, such as spectral content, can be computed, not just the effects of the scintillation on GPS receiver performance. The majority of the current wide bandwidth monitoring is being done using a commercially off-the-shelf GPS receiver implemented with special software -- the GSV4000 GPS Ionospheric Scintillation Monitor (GISM) and predecessor prototype units. Now, GPS Silicon Valley is pleased to offer the new GSV4004 GPS Ionospheric Scintillation and TEC Monitor (GISTM) receiver. This receiver, a NovAtel EURO4 dual-frequency receiver with special firmware, comprises the major component of a GPS signal monitor, specifically configured to measure amplitude and phase scintillation from the L1 frequency GPS signals, and the ionosphere's TEC from the L1 and L2 frequency GPS signals. This scintillation and TEC monitoring receiver is housed in a NovAtel GPStation4E housing with a low phase noise oscillator, and provides true amplitude, single frequency carrier phase measurements and TEC measurements of up to 11 GPS satellites in view. It will also track one SBAS (WAAS, EGNOS or MSAS) satellite, providing L1 measurements and data, as the 12th satellite. The unit comes with complete software that allows the automatic measurement and computation of all the major scintillation parameters and TEC. A variety of antennae, with or without choke rings and cables, are offered as options. In this paper, the wide bandwidth monitoring capabilities of these receivers are described. This is followed by the presentation of data collected from a selection of recorded scintillation events and TEC calibration results.

Journal ArticleDOI
TL;DR: In this article, the authors presented data from first global positioning system (GPS) measurements of global response of the ionosphere to solar flares of September 23, 1998 and July 29, 1999 and found that fluctuations of total electron content (TEC) obtained by removing the linear trend of TEC with a time window of about 5 min, are coherent for all stations and the line of sight to the GPS satellites on the dayside of the Earth.

Journal ArticleDOI
TL;DR: A pivotal function for the formation of a Tec/Vav1/PRLr complex during PRL-driven signal transduction is suggested, given the role of Vav1 in the control of cell proliferation and the regulation of Rho family-mediated cytoskeletal alterations.
Abstract: Stimulation of the PRL receptor (PRLr) results in the activation of the guanine nucleotide exchange factor (GEF) p95 Vav1 with corresponding alterations in cytoarchitecture and cell motility. To better understand the mechanisms involved in the regulation of Vav1 activity, the role of the tyrosine kinase p70 Tec was examined. Coimmunoprecipitation and in vitro kinase assays revealed that ligand stimulation of the PRLr resulted in the rapid activation of Tec and its concomitant association with the PRLr. When coexpressed in COS-1 cells, both Vav1 and Tec were found to associate with the PRLr in the presence of ligand. In the absence of receptor, a constitutive complex between Vav1 and Tec was noted. Both Vav1 and Tec, however, were capable of independent engagement of a bipartite intracellular domain of the PRLr. Deletion mapping studies confined this interaction to residues 323 to 527 of the intracellular domain of the PRLr. Furthermore, Tec enhanced the GEF activity of Vav1 as evidenced by an increase in GTP-bound Rac1. These data would suggest a pivotal function for the formation of a Tec/Vav1/PRLr complex during PRL-driven signal transduction, given the role of Vav1 in the control of cell proliferation and the regulation of Rho family-mediated cytoskeletal alterations. (Molecular Endocrinology 15: 832‐841, 2001)

Journal ArticleDOI
TL;DR: This paper used the TOPEX satellite data set to compare climatologies of total electron content (TEC) measurements from the dual-frequency altimeter with the Doppler orbitography and radiopositioning integrated by satellite (DORIS) TEC results and with the international reference ionosphere (IRI and Bent model predictions corresponding to the satellite measurements.
Abstract: We have used the TOPEX satellite data set to compare climatologies of total electron content (TEC) measurements from the dual-frequency altimeter with the Doppler orbitography and radiopositioning integrated by satellite (DORIS) TEC results and with the international reference ionosphere (IRI) and Bent model predictions corresponding to the satellite measurements. We have used the TOPEX measurements from launch in 1992 through 1997 to build a database that includes time, geographic and geomagnetic coordinates of the measurement, geomagnetic indices (Kp, previous Kp, hemispheric power, and integral of hemispheric power over the previous 36 hours), solar index (F 10.7), TOPEX and DORIS TEC measurements, and empirical model (IRI and Bent) results corresponding to the TOPEX measurements. We have binned the measurements in a magnetic local time, magnetic latitude coordinate system to produce global maps of TEC. We present climatological differences between TOPEX TEC and DORIS measurements as well as between TOPEX and the two empirical models. The maps were constructed using all appropriate TOPEX TEC data, DORIS TEC measurements, and the corresponding Bent and IRI model results for solar minimum conditions.

01 Jan 2001
TL;DR: Activated T cells may enhance IL-15 expression in two ways: by secreting IFN-gamma and by cell to cell contact using CD154, each signal alone induces IL- 15 in similar magnitudes, and both signals are additive.
Abstract: Interleukin-15 (IL-15) is a potent T-cell growth fac- tor and activator. Acute rejection of kidney allografts strongly correlated with elevated IL-15 mRNA in the graft. A role in the rejection process is also suggested for the interaction between CD40 ligand (CD154) expressed on membranes of activated T cells and its receptor (CD40). The effect of CD40 ligation on IL-15 production in human tubular epithelial cells (TEC) was investigated. TEC were co-cultured with a cell line genetically engineered to express CD154. CD154-expressing cells (CD40L cells) bind to TEC. Addition of the CD40L cells to the TEC culture resulted in elevated IL-15 levels. This enhanced production was not observed with control cells, when anti- CD154 antibody was added, and when direct contact between CD40L-cells and TEC was prevented with the use of a Trans- well system. CD40 activation resulted in a twofold increase of IL-15 mRNA transcripts as measured by reverse transcription- PCR and a concordant elevation in IL-15 protein production as measured by specific enzyme-linked immunosorbent assay. The intensity of activation by CD154 was linearly dependent on cell number, reaching plateau when the effector/target-ratio was 1:1. The increase of IL-15 levels was similar to that produced by stimulation with interferon-g (IFN-g). Combina- tion of IFN-g and activation with CD154 resulted in an addi- tive effect. To conclude, activated T cells may enhance IL-15 expression in two ways: by secreting IFN-g and by cell to cell contact using CD154. Each signal alone induces IL-15 in similar magnitudes, and both signals are additive. Because IL-15 is a major T-cell activator, this interaction may contrib- ute to graft rejection.

Journal ArticleDOI
TL;DR: In this paper, the authors analyzed the polarization properties of very high frequency (VHF) pulses generated by the Portable Pulser (LAPP) and found that the polarization degrades from circular toward elliptical as the nadir angle increases.
Abstract: Coherent very high frequency (VHF) radio observations with the pair of orthogonal log-periodic array antennas of the FORTE satellite allow us to study thoroughly the polarization properties for a received signal. Eighty-one broadband VHF pulses that were generated by the Los Alamos Portable Pulser (LAPP) have been analyzed. The data are analyzed by computing the Stokes parameters in the time-frequency domain. We first examine the LAPP pulses at high time resolution so as to separate the ordinary and extraordinary ionospheric modes. The two modes have been found to be mirror images of each other in terms of polarization, as would be expected. For each mode the polarization degrades from circular toward elliptical as the nadir angle increases. Antenna pattern effects on this observation are discussed. The tilt of the detected polarization ellipse is found to be tightly associated with the azimuthal direction of the pulse source. The same set of data are then examined with much lower time resolution to intentionally mix together the two split modes, so that the ionospheric Faraday rotation can be detected. With the known geomagnetic field the total electron content (TEC) is computed, which shows good agreement with the TEC computed by dechirping the signal. A case study of an impulsive lightning emission shows that it is highly polarized, indicating that the associated breakdown processes are highly coherent and organized. Finally, we discuss the potential use of the polarization observations for locating terrestrial radio signals.

Journal ArticleDOI
TL;DR: In this paper, the effect of CD40 ligand ligation on IL-15 production in human tubular epithelial cells (TEC) was investigated, and it was shown that activation with CD40-expressing cells (CD40L cells) resulted in an additive effect.
Abstract: Interleukin-15 (IL-15) is a potent T-cell growth factor and activator. Acute rejection of kidney allografts strongly correlated with elevated IL-15 mRNA in the graft. A role in the rejection process is also suggested for the interaction between CD40 ligand (CD154) expressed on membranes of activated T cells and its receptor (CD40). The effect of CD40 ligation on IL-15 production in human tubular epithelial cells (TEC) was investigated. TEC were co-cultured with a cell line genetically engineered to express CD154. CD154-expressing cells (CD40L cells) bind to TEC. Addition of the CD40L cells to the TEC culture resulted in elevated IL-15 levels. This enhanced production was not observed with control cells, when anti-CD154 antibody was added, and when direct contact between CD40L-cells and TEC was prevented with the use of a Trans-well system. CD40 activation resulted in a twofold increase of IL-15 mRNA transcripts as measured by reverse transcription-PCR and a concordant elevation in IL-15 protein production as measured by specific enzyme-linked immunosorbent assay. The intensity of activation by CD154 was linearly dependent on cell number, reaching plateau when the effector/target-ratio was 1:1. The increase of IL-15 levels was similar to that produced by stimulation with interferon-gamma (IFN-gamma). Combination of IFN-gamma and activation with CD154 resulted in an additive effect. To conclude, activated T cells may enhance IL-15 expression in two ways: by secreting IFN-gamma and by cell to cell contact using CD154. Each signal alone induces IL-15 in similar magnitudes, and both signals are additive. Because IL-15 is a major T-cell activator, this interaction may contribute to graft rejection.

Journal ArticleDOI
TL;DR: In this article, the authors explore the total electron content (TEC) registered by Global Positioning System (GPS) receivers located close to the Cornell All-Sky Imager (CASI) at the Arecibo Observatory.
Abstract: On November 22–23, 1997, a geomagnetic storm occurred during a period of excellent viewing conditions over the Arecibo Observatory in Puerto Rico. Here we explore the total electron content (TEC) registered by Global Positioning System (GPS) receivers located close to the Cornell All-Sky Imager (CASI) at the Arecibo Observatory. The storm began with the equatorward surge of a very high (100% increase) TEC enhancement stretching for many hours of local time on the dayside. At dusk the TEC over the Caribbean remained elevated with levels equal to the noontime monthly averages. During the event the TEC was highly structured and clearly correlated with high and low airglow emission levels. In one fortuitous instance a common ionospheric penetration point (15 km apart), shared by two GPS satellites viewed from two receiving stations, registered an 8 TEC unit difference during the active period. We show that a GPS station can be calibrated using the pseudorange method and a reliable data-driven technique during quiet conditions and still have absolute TEC capability within 2 TEC units (RMS) 5 days later. We compare the observations to a climatological model which, although reasonable for quiet times, is very poor during the storm period. We also present an independent evaluation of the GPS TEC. This study is an initial step toward quality control of this database, needed before it is used in an assimilation model.

Journal ArticleDOI
TL;DR: In this article, the results from the vertical ionosondes located under the path of totality and in the partial eclipse region and dual frequency GPS TEC measurements are discussed in relation to making model predictions.
Abstract: The localised “night” created as the moon's shadow travelled across the Earth during the total solar eclipse of 11th August 1999, produced changes in the ionosphere across Europe that were monitored with a variety of modern instrumentation. The passage of the 100km wide, super-sonic lunar shadow offered the opportunity to examine the changes in electron densities, radio absorption, neutral wind patterns and the possible generation of waves in the layers of the ionosphere. All these for an event for which the cause of the disturbance can be calculated with accuracy. Reported here are the results from the vertical ionosondes located under the path of totality and in the partial eclipse region and dual frequency GPS TEC measurements. The ionosondes showed that even in the partial shadow the peak electron densities of the F & E ionospheric layers decreased by as much as 20–35%. The TEC measurements showed that the vertical equivalent line integrated electron density dropped by 15% at the 97% partial eclipse north of the path of totality. The consequences of these observations are discussed in relation to making model predictions.

Journal ArticleDOI
TL;DR: In this article, the authors derived the transfer function for a spherically symmetric ionosphere with an arbitrary radial electron density profile in the limit where the radio frequencies of interest are much larger than the plasma frequency pe 2 / 2 )i s carried out.
Abstract: The ionospheric transfer function is derived for a spherically symmetric ionosphere with an arbitrary radial electron density profile in the limit where the radio frequencies of interest are much larger than the plasma frequency pe .A n expansion of the transfer function to second order in the parameter X ( pe 2 / 2 )i s carried out. In this limit the dispersive properties of the ionosphere are manifested as a frequency-dependent time of arrival that includes quadratic, cubic, and quartic terms in 1/. The coefficients of these terms are related to the total electron content (TEC) along the slant path from transmitter to receiver, the product of TEC and the longitudinal magnetic field strength along the slant path, and refractive bending and higher-order electron density profile effects, respectively. By fitting the time of arrival versus frequency of a transionospheric signal to a polynomial in 1/ it is possible to extract the TEC, the longitudinal magnetic field strength, the peak electron density, and an effective thickness for the ionosphere. This exercise was carried out for a number of transionospheric pulses measured in the VHF by the FORTE satellite receiver and generated by the Los Alamos Portable Pulser. The results are compared with predictions derived from the International Reference Ionosphere and the United States Geological Survey geomagnetic field model.

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TL;DR: In this paper, a simple ionospheric model consisting of a vertical TEC, a horizontal gradient, and the azimuth of that gradient was developed and fitted to the GPS Total Electron Content (TEC) data from each receiver.
Abstract: : We have conducted an experiment to evaluate the usefulness of ionospheric data produced using the Global Positioning System (GPS) for making Faraday rotation and interferometer phase corrections at the NRAO Very Large Array (VLA). Four GPS receivers were installed at the VLA site -- one at the array center and one at the end of each arm. A simple ionospheric model consisting of a vertical TEC, a horizontal gradient, and the azimuth of that gradient was developed and fitted to the GPS Total Electron Content (TEC) data from each receiver. The model was then used to predict the TEC in the observing direction. Ionospheric Faraday rotation and phase gradients were then estimated and compared with VLA measurements taken at frequencies of 322 and 333 MHz. We find that we can normally make Faraday rotation corrections that are accurate to less than or approximately 2 deg, although one unexplained discrepancy remains. The interferometer phase shifts caused by large-scale (greater than or approx. 1000 km) ionospheric structures can be predicted by our model. However the phase shifts caused by smaller (less than or approx. 100 km) structures can be estimated only when the direction of observation lies within a few degrees of one of the GPS satellites.

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TL;DR: In this article, the AIM1.03L-R ionospheric model is used to estimate the induced plasma drift in the meridional neutral wind (MNE) in both local and regional forms.
Abstract: Assimilation Ionosphere Model (AIM) is a physics-based, global, ionospheric specification model that is currently under development. It assimilates a diverse set of real-time (or near-real-time) measurements, such as ionograms, GPS slant total electron content (TEC), and in situ plasma measurements. This study focuses on a middle latitude ionosonde assimilation capability in both local and regional forms. The models described are capable of using theƒ0F2 and hmF2 from ionograms to generate either a local or a regional distribution of the induced plasma drift. This induced drift is usually caused by the meridional neutral wind. Results from a local model (AIM1.03L) and a regional model (AIM1.03R) are presented and compared with the international reference ionosphere (IRI) climatological predictions as well as GPS slant TEC measurements. Results from year-long studies during solar maximum show that the accuracy of the AIM1.03L model is about a factor of 2 better than that of IRI. An initial month-long regional study is also presented, and the results are almost as good. A study is also carried out using observations taken during the Combined Ionospheric Campaign (CIC) held in November, 1997, in the Caribbean. The digisonde located at Ramey Solar Observatory is used to drive the AIM1.03L model, and the predicted GPS slant TECs are compared to those observed by a GPS receiver located at St. Croix. This study confirms that this first step in preparing a weather-sensitive ionospheric representation is superior to a climatological representation. This sets the stage for the development of full assimilation of GPS TEC, in situ density measurements, etc., and it is anticipated that the AIM1.03L-R ionospheric representation will provide an accurate ionospheric specification.

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TL;DR: In this article, the authors compared the IRI-95 model with the observed and the predicted TEC and found that the discrepancies are maximum in equinox and summer months and minimum in winter months.

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TL;DR: In this article, an analysis of the ionospheric response to solar flares was performed using phase measurements of the total electron content (TEC) in the ionosphere using an international GPS network.