Showing papers on "Termitomyces clypeatus published in 2016"
TL;DR: It is demonstrated that AkP could be an effective biomolecule for killing of cancer cells by p53 restoration and surface proteoglycans cleavage by way of serine protease inhibitor phenyl methane sulfonyl fluoride.
20 citations
TL;DR: Termitomyces clypeatus possesses anticancer activity, valuable for application in food and drug products, which might be due to the antioxidant effects on EAC bearing hosts.
Abstract: Context: Termitomyces clypeatus (Lyophyllaceae) is a filamentous edible mushroom, having ethnomedicinal uses. However, information about the antioxidant, anticancer and antitumour properties of this mushroom remains to be elucidated.Objective: The study examines the in vitro antioxidant, anticancer and in vivo antitumour activity of T. clypeatus.Materials and methods: Antioxidant activity was evaluated with seven in vitro assays. Cytotoxicity of T. clypeatus was tested against a panel of cancer cells lines including U373MG, MDA-MB-468, HepG2, HL-60, A549, U937, OAW-42 and Y-79 using MTT assay. The antitumour activity of aqueous extract was evaluated against Ehrlich ascites carcinoma (EAC) tumour model in Swiss albino mice.Results: HPLC analysis of aqueous extract revealed the presence of sugar entities. Termitomyces clypeatus showed excellent in vitro antioxidant activity. Termitomyces clypeatus was found cytotoxic against all cancer cells, among which it showed higher activity against U937 (IC50 ...
16 citations
01 Jan 2016
TL;DR: The results indicate that T. clypeatus contains biologically-active compounds that could induce teratogenicity in zebrafish embryos.
Abstract: This work presents the teratogenic activity of the termite mound mushroom Termitomyces clypeatus extract on Danio rerio embryos Hatched embryos treated with 01% and higher concentration of the mushroom extract significantly yielded higher mortality rate and delayed growth than the control embryos The hatchability rate of embryos treated with 005% and higher concentrations of T cleaptus extract was found to be significantly lower than that of the control Apparently, the effect of extract to embryos is dose-dependent The different phenotypic endpoints of embryos observed include wavy somite embryo, unhatched embryo with twisted tail tip, delayed development of embryo (still at segmentation phase), tail malformed embryo and coagulated embryo Our results indicate that T clypeatus contains biologically-active compounds that could induce teratogenicity in zebrafish embryos
9 citations
Journal Article•
TL;DR: Antioxidant potential inedible wild mushrooms are found to be on account of combinations of biochemicals, rather than any such significant individual components as TPC, AA, or alkaloid.
Abstract: Objective: The main objective of this research was to analyze some selected indigenous wild edible mushrooms in Eastern India for their novel antioxidant components and their properties specifically used by primitive tribal groups of Eastern India. Methods: The antioxidant components were analyzed by standardized spectrophotometric methods. The antioxidant properties were analyzed by DPPH Free radical scavenging & Reducing power ability assay. Results: The TPC (phenolic content) in the studied edible mushroom varied from 4.55 mg/g ( Russula nigricans ) to 0.9 mg/g GAE ( Lentinus tuberigium) . Measured in term of antioxidants Termitomyces group ranked higher than Russula and Volvariella sp. The scavenging effect of studied mushrooms on 1,1 DPPH varied from 61% to as high as 94%. On the other hand, reducing power (RPA) in methanolic extracts were in the order of T. clypeatus (4.21) T. heimi (2.20) ~ R. breviceps (1.73) ~ Termitomyces eurrhizus (1.11) ~ T. rufum (1.07). Antioxidant potential inedible wild mushrooms are found to be on account of combinations of biochemicals, rather than any such significant individual components as TPC, AA, or alkaloid. Conclusion: This is for the first time wild edibles such as Termitomyces clypeatus, Termitomyces eurrhizus, Termitomyces heimii, Russula brevipes, Tuber rufum, Russula nigricans, Volvariella volvaceae, Lentinus fusipes, Lentinus tuberigium and R. lepida from eastern India were observed, collected and subjected to nutritional and biochemical analysis. Of significance is the identification of Tuber rufum and Volvariella volvaceae growing wild as edible mushrooms which have not been profiled in the Indian context. The analysed mushroom especially Lentinus fusipes and Lentinus tuberigium was found valuable in terms of iron and calcium, besides having useful phytochemicals such as phenolics, ascorbic acid, carotenoids. Keywords: Deciduous forests, Orissa, Wild food, Phenolics, Ergosterol, Termitomyces
6 citations
TL;DR: Correlation between extracellular protein productions of the fungus with respect to its specific growth rate established that secreted proteins were produced most efficiently at low specific growth rates.
6 citations
01 Jan 2016
TL;DR: The analysed mushroom especially Lentinus fusipes and Lentinu tuberigium was found valuable in terms of iron and calcium, besides having useful phytochemicals such as phenolics, ascorbic acid, carotenoids.
Abstract: Objective: The main objective of this research was to analyze some selected indigenous wild edible mushrooms in Eastern India for their novel antioxidant components and their properties specifically used by primitive tribal groups of Eastern India. Methods: The antioxidant components were analyzed by standardized spectrophotometric methods. The antioxidant properties were analyzed by DPPH Free radical scavenging & Reducing power ability assay. Results: The TPC (phenolic content) in the studied edible mushroom varied from 4.55 mg/g (Russula nigricans) to 0.9 mg/g GAE (Lentinus tuberigium). Measured in term of antioxidants Termitomyces group ranked higher than Russula and Volvariella sp. The scavenging effect of studied mushrooms on 1,1 DPPH varied from 61% to as high as 94%. On the other hand, reducing power (RPA) in methanolic extracts were in the order of T. clypeatus (4.21) T. heimi (2.20) ~R. breviceps (1.73) ~ Termitomyces eurrhizus (1.11) ~ T. rufum (1.07). Antioxidant potential inedible wild mushrooms are found to be on account of combinations of biochemicals, rather than any such significant individual components as TPC, AA, or alkaloid. Conclusion: This is for the first time wild edibles such as Termitomyces clypeatus, Termitomyces eurrhizus, Termitomyces heimii, Russula brevipes, Tuber rufum, Russula nigricans, Volvariella volvaceae, Lentinus fusipes, Lentinus tuberigium and R. lepida from eastern India were observed, collected and subjected to nutritional and biochemical analysis. Of significance is the identification of Tuber rufum and Volvariella volvaceae growing wild as edible mushrooms which have not been profiled in the Indian context. The analysed mushroom especially Lentinus fusipes and Lentinus tuberigium was found valuable in terms of iron and calcium, besides having useful phytochemicals such as phenolics, ascorbic acid, carotenoids.
5 citations
Patent•
13 Jan 2016
TL;DR: In this article, a reference gene for molecular identification of Termitomyces clypeatus and a molecular identification method for fungus species identification has been proposed, which relates to the field of fungus identification.
Abstract: The invention belongs to the field of fungus species identification and relates to a reference gene for molecular identification of Termitomyces clypeatus and a molecular identification method. Sequences of adopted primers are RPB2B-f: 5'-CCGCAAAGGCTGGTGTATC-3' and RPB2B-r: 5'-TTCGAATGAGTTCAAGTGT-3', and the primers can be used for amplification of large-subunit gene (RPB2) of RNA (ribonucleic acid) polymerase II of the termitomyces clypeatus to obtain a reference detection gene. The termitomyces clypeatus can be quickly and accurately identified according to RPB2 gene DNA (deoxyribonucleic acid) sequence differences. The RPB2 gene overcomes the defect of difficulty in identification of traditional termitomyces clypeatus forms and has the advantages of universality and easiness in amplification and comparison, reliability and accuracy in identification are greatly improved, and a powerful research implement is provided for excavation, protection and utilization of genetic resources of the termitomyces clypeatus.
3 citations
TL;DR: In this paper, the phytochemical screening and antioxidant properties of the ethanolic extract of Termitomyces clypeatus were evaluated through superoxide radical scavenging assay, DPPH radical scavengence assay, chelating ability, reducing power and total antioxidant capacity determining assays.
Abstract: Article history: Received on: 11/03/2016 Revised on: 22/04/2016 Accepted on: 01/06/2016 Available online: 28/07/2016 The present study documents the phytochemical screening and antioxidant properties of ethanolic extract of Termitomyces clypeatus. Phytochemical constituents like phenols, flavonoids and ascorbic acid were much higher than β carotene and lycopene. The analysis of the phenolic compounds performed by HPLC, revealed the presence of pyrogallol (0.223 μg/mg) and cinnamic acid (0.095 μg/mg). Antioxidant activity was evaluated through superoxide radical scavenging assay, DPPH radical scavenging assay, chelating ability, reducing power and total antioxidant capacity determining assays. Their EC50 values ranged from 0.21 μg/ml to 3.22 mg/ml. Result implies that T. clypeatus can not only serve as a food supplement but also be used as treatment for various oxidative stress related diseases.
3 citations