Topic
Terpene
About: Terpene is a research topic. Over the lifetime, 2208 publications have been published within this topic receiving 51480 citations. The topic is also known as: terpenes.
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TL;DR: A novel class of volatile fungal sesquiterpenes as well as the gene cluster involved in their biosynthesis is identified, identified and demonstrated that this gene cluster is involved in the synthesis of volatile terpene compounds.
67 citations
TL;DR: The results indicate that cytochromes P-450 induced by a synthetic compound, phenobarbital, may have originally evolved in response to terpenoid compounds normally present in the environment.
67 citations
TL;DR: The results support the notion that the biosynthesis of a desired terpene can be dramatically improved by directing that metabolism to a non-native cellular compartment, thus avoiding regulatory mechanisms that might attenuate carbon flux within an engineered pathway.
Abstract: Terpenes comprise a distinct class of natural products that serve a diverse range of physiological functions, provide for interactions between plants and their environment and represent a resource for many kinds of practical applications. To better appreciate the importance of terpenes to overall growth and development, and to create a production capacity for specific terpenes of industrial interest, we have pioneered the development of strategies for diverting carbon flow from the native terpene biosynthetic pathways operating in the cytosol and plastid compartments of tobacco for the generation of specific classes of terpenes. In the current work, we demonstrate how difficult it is to divert the 5-carbon intermediates DMAPP and IPP from the mevalonate pathway operating in the cytoplasm for triterpene biosynthesis, yet diversion of the same intermediates from the methylerythritol phosphate pathway operating in the plastid compartment leads to the accumulation of very high levels of the triterpene squalene. This was assessed by the co-expression of an avian farnesyl diphosphate synthase and yeast squalene synthase genes targeting metabolism in the cytoplasm or chloroplast. We also evaluated the possibility of directing this metabolism to the secretory trichomes of tobacco by comparing the effects of trichome-specific gene promoters to strong, constitutive viral promoters. Surprisingly, when transgene expression was directed to trichomes, high-level squalene accumulation was observed, but overall plant growth and physiology were reduced up to 80 % of the non-transgenic controls. Our results support the notion that the biosynthesis of a desired terpene can be dramatically improved by directing that metabolism to a non-native cellular compartment, thus avoiding regulatory mechanisms that might attenuate carbon flux within an engineered pathway.
67 citations
TL;DR: Synthesis of cis-verbenol, which is not a sex-specific pheromone, from the host plant terpene (–)-α-pinene and other metabolites from these two terpenes was not inhibited by the antibiotic.
Abstract: Ingestion of diet containing streptomycin inhibited the conversion of myrcene, a host plant terpene, to the male-specific pheromones ipsenol and ipsdienol in 1ps paraconfusus. Synthesis of cis-verbenol, which is not a sex-specific pheromone, from the host plant terpene (-)-alpha-pinene and other metabolites from these two terpenes was not inhibited by the antibiotic.
67 citations
TL;DR: It is found that limonene directly binds to the adenosine A(2A) receptor, which may induce sedative effects, and results from an in vitro radioligand binding assay showed thatLimonene exhibits selective affinity to A( 2A) receptors.
67 citations