Theobromine

About: Theobromine is a research topic. Over the lifetime, 1137 publications have been published within this topic receiving 29723 citations. The topic is also known as: 3,7-Dimethylxanthine & Theobromin.

Metabolic Profiling of Dietary Polyphenols and Methylxanthines in Normal and Malignant Mammary Tissues from Breast Cancer Patients

Abstract: Scope Dietary polyphenols may protect against breast cancer. However, it is unknown whether polyphenols reach human malignant breast tumors in molecular forms and(or) at concentrations likely to act against cancer. Methods and results Ninteen breast cancer patients consumed three capsules daily from biopsy-confirmed diagnosis to surgery (6 ± 2 days). The capsules contained pomegranate, orange, lemon, olive, cocoa, and grapeseed extracts plus resveratrol, providing 37 different phenolics (473.7 mg), theobromine and caffeine (19.7 mg). A total of 101 metabolites are identified in urine, 69 in plasma, 39 in normal (NT), and 33 in malignant (MT) tissues by UPLC-ESI-QTOF-MS. Eight control patients did not consume extracts. Phenolic-derived metabolites in MT and NT are mainly glucuronidated and(or) sulfated. Some representative metabolites detected in MT (median and range, pmol g-1 ) are urolithin-A-3-O-glucuronide (26.2; 3.2-66.5), 2,5-dihydroxybenzoic acid (40.2; 27.7-52.2), resveratrol-3-O-sulfate (86.4; 7.8-224.4), dihydroresveratrol-3-O-glucuronide (109.9; 10.3-229.4), and theobromine (715.0; 153.9-3,216). Metabolites, as detected in breast tissues, do not exert antiproliferative or estrogenic/antiestrogenic activities in MCF-7 breast cancer cells. Conclusion This is the first study that describes the metabolic profiling of dietary phenolics and methylxanthines in MT and NT comprehensively. Although phase-II conjugation might hamper a direct anticancer activity, long-term tumor-senescent chemoprevention cannot be discarded.

Evaluation of xanthine derivatives in chocolate – nutritional and chemical aspects

Abstract: Organic substances containing nitrogen are widespread throughout the whole of the natural world. Also included amongst these are the methylxanthine derivatives caffeine, theobromine and theophylline. These are very closely related and are to be found in extremely varying contents in different plants. Because of their pharmacological effect, which is fundamentally of a stimulative nature, methylxanthines have been significant since time immemorial as luxury, non-essential foodstuffs and as medication. These substances are often referred to as alkaloids. Theobromine is the major alkaloid in cacao (Theobroma cacao). Caffeine, on the other hand, is only to be found in cacao in very small quantities and theophylline only in trace amounts. Methylxanthines are said to contribute towards the typically bitter taste of cacao. In addition they represent important analytical parameters with regard to the evaluation of the quality of cacao-containing and chocolate products. The amount of methylxanthines in cacao depends on various influencing factors, the most essential ones being processing procedures, genotype, geographical origin and cacao bean weight. By determining the amount of theobromine and caffeine in cacao and/or cacao products, the amount of fat-free dry cacao can also be estimated. There are various methods available for the detection and determination of methylxanthines. UV-spectrophotometric determination and analysis using HPLC have attained major significance here. With regard to human nutrition, cacao and cacao products are the main natural sources of theobromine, but significantly less so with regard to caffeine. The presence of methylxanthines in cacao products leads to that they are sometimes the subject of consumer criticism, particularly because they are considered as luxury foodstuffs. The concluding evaluation of methylxanthines by dieticians and toxicologists reveals that plants that contain methylxanthines have been consumed for as long as can be remembered without having led to damage to health. Scientific research has shown that the consumption of these substances has no consequence whatsoever, as long as consumption is not excessive, which is the case for all foodstuffs. Methylxanthines in cacao products have no negative effects on the health of humans because their amounts in chocolates and other processed foodstuffs containing cacao are so low that they account for only a very small proportion of the whole of the human diet.