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Showing papers on "Thin-layer chromatography published in 1967"


Journal ArticleDOI
TL;DR: Concentrations of the four glycosyl ceramides in plasma and in erythrocytes were determined for samples from young, healthy males and varied with the complexity of the oligosaccharide moiety and ranged from 94% with glucosyl ceramide to 71% with globoside.

642 citations


Journal ArticleDOI
TL;DR: Several crystalline N-hydroxysuccinimide esters of short- and long-chain fatty acids have been synthesized and the behavior of the N-acylamino acids on thin-layer chromatography is described.

302 citations


Journal ArticleDOI
TL;DR: A simple, rapid, and reliable technique for identifying small amounts of phenylthiohydantoin (PTH) derivatives has been developed for use in the analysis by Edman degradation ofsmall amounts of peptides isolated from fingerprints.

291 citations


Journal ArticleDOI
TL;DR: A two-dimensional thin-layer chromatographic system that separates all of the known polar lipids of milk and mammary tissue is described.

182 citations


Book ChapterDOI
TL;DR: This chapter discusses the combined methodology for the isolation and determination of polar lipids from mitochondria and submitochondrial preparations, used for the most part with mitochondria isolated from animal organs, particularly those from heart, kidney, liver, and brain.
Abstract: Publisher Summary This chapter discusses the combined methodology for the isolation and determination of polar lipids from mitochondria and submitochondrial preparations. Procedures are presented for both micro scale and somewhat larger scale work. Included are extraction, general laboratory techniques, removal of nonlipid contaminants from lipid extracts, isolation by diethylaminoethyl (DEAE) cellulose column chromatography, quantitative analysis by thin layer chromatography, and procedures for characterization of lipids. The procedures have been used for the most part with mitochondria isolated from animal organs, particularly those from heart, kidney, liver, and brain. principal polar lipid classes: phosphatidylcholine (lecithin), phosphatidylethanolamine, and diphosphatidylglyeerol (cardiolipin). The structures of these three lipids are schematically presented. Mitochondrial polar lipids are very susceptible to alteration. Elution from DEAE columns in the characteristic manner, migration to the proper positions by two-dimensional thin layer chromatography, and reactivity to specific spray reagents provided the basis for characterization of mitochondrial phospholipids. Chromatographic identifications can be confirmed with lipids isolated by DEAE column or thin layer chromatography by pressing with potassium bromide into a pellet for infrared examination or by hydrolysis with acid or base followed by paper and thin layer chromatography for separation of hydrolysis products.

179 citations


Journal ArticleDOI
TL;DR: Three one-dimensional thin-layer Chromatographie systems for separation of acidic phospholipids such as cardiolipin, phosphatidic acid, ceramidemonohexosides, sulfatides and ceramidedihexoside (overlapping) are described and demonstrated on total lipid extracts from animal tissues.

128 citations


Journal ArticleDOI
TL;DR: Five new solvent systems are reported for the separation of 1-dimethylaminonaphthalene-5-sulphonylamino acids by thin-layer chromatography on silica gel and a method for the semiquantitative estimation of amino acids in biological fluids is described.
Abstract: 1. Five new solvent systems are reported for the separation of 1-dimethylaminonaphthalene-5-sulphonylamino acids by thin-layer chromatography on silica gel. After two-dimensional chromatography with a suitable pair of these solvent systems, most of the 1-dimethylaminonaphthalene-5-sulphonyl derivatives were completely separated and could be located by their intense yellow fluorescence when viewed under u.v. light. 2. These techniques have been used to identify 21 amino acids present in superfusates of cat cerebral cortex, plasma and cerebrospinal fluid. 3. A method for the semiquantitative estimation of amino acids in biological fluids is described in which the fluorescent intensity of their separated 1-dimethylaminonaphthalene-5-sulphonyl derivatives was measured.

123 citations


Journal ArticleDOI
TL;DR: The lipids from isolated human sebaceous glands have been studied by thin- layer chromatography and no thin-layer chromatographic spots were found that corresponded to the reference standards of hydrocarbon, sterol esters, free fatty acids, sterols, monoglycerides or diglycerides.
Abstract: The lipids from isolated human sebaceous glands have been studied by thin-layer chromatography. Lipids were present that corresponded to the reference standards of squalene, wax esters, and triglycerides. No thin-layer chromatographic spots were found that corresponded to the reference standards of hydrocarbon, sterol esters, free fatty acids, sterols, monoglycerides or diglycerides.

97 citations


Journal ArticleDOI
TL;DR: The results of chromatography of carbohydrates on thin layers of silica gel in the presence of sodium mono- and dihydrogen phosphate are often better than those of paper chromatography.

92 citations


Journal ArticleDOI
TL;DR: Study of the substrate specificity, heat stability, pH optimum, and chromatographic behavior of the chloramphenicol-acetylating enzyme from the multiple drug-resistant E. coli found it was not possible to decide whether one or two enzymes participate in the acetylation of chlorampshenicol.

87 citations


Journal ArticleDOI
TL;DR: The technique is based on preliminary separation of total lipids on thin layer plates of silicic acid with a second stage column chromatography and allows the complete recovery of intact single lipids from naturally occurring mixtures.




Journal ArticleDOI
Gösta Arvidson1
TL;DR: The four fractions obtained by argentation thin-layer chromatography of intact rat liver lecithins can be further subdivided by reversed-phase partition thin- layer chromatography on hydrophobic kieselguhr.

Journal ArticleDOI
21 Jan 1967-Nature
TL;DR: The use of silica gel partition columns has been investigated in this paper for the separation of the various gibberellins from each other, but there appears to be no report of the use of such columns for the preparation of the compounds.
Abstract: ALTHOUGH there are many chromatographic methods for separating the various gibberellins from each other, there appears to be no report of the use of silica gel partition columns. Columns are more useful for preparative separations than thin layer and paper chromatographic methods. Methods using absorption columns made with charcoal and silicic acid have been described1,2. Partition columns are usually considered to provide especially mild conditions for chromatography, which may be important with compounds that tend to be somewhat labile, such as the gibberellins.

Journal ArticleDOI
TL;DR: Two one-dimensional systems for separation of glycolipids from total lipid extracts of tissues by thin-layer chromatography are described and all neutral lipids moved to the very top of the chromatogram and phospholipids stayed at the origin.

Journal ArticleDOI
TL;DR: Adequate specificity of the method was shown by no significant change in the values when aliquots of all samples estimated were further purified by acid hydrolysis of the diacetate to the 21-monoacetate followed by paper chromatography, and then after oxidation of the monoacet...
Abstract: A double isotope derivative method suitable for the estimation of aldosterone in human peripheral plasma has been developed. 3H-acetic anhydride was used as labeled reagent (1050 mc/mmole) and 4-14C-aldosterone (46 mc/mmole; 0.8 mμg added to the sample) as indicator. Only 0.25 μl (2.5 mc) acetic anhydride in 5μl benzene is required per reaction as aldosterone 21-monoacetate is the initial derivative formed. Purification is achieved by 2-dimensional silica gel thin layer chromatography of aldosterone 21-monoacetate and of the 18,21-diacetate then formed using nonisotopic acetic anhydride, followed by 2 paper chromatograms of the diacetate. The over-all recovery of the method with these 4 chromatographic steps is 31 ±9 (sd)%. Adequate specificity of the method was shown by no significant change in the values when aliquots of all samples estimated were further purified by acid hydrolysis of the diacetate to the 21-monoacetate followed by paper chromatography, and then after oxidation of the monoacet...

Journal ArticleDOI
TL;DR: In this paper, a method for the characterization of more than sixty nucleo-derivatives on purified cellulose layers using n -propanol-25 % ammonia-water (6:3:1, v/v) in the first dimension and isopropanol-saturated ammonium sulphate water (2:79:19, V/v), in the second dimension.

Journal ArticleDOI
TL;DR: Crude ether extracts of green shoots of Cucumis sativus L. promoted the elongation of cucumber hypocotyl segments and identified the growth promoter as indole-3-ethanol by mass spectrometry, thin layer and gas chromatography, and ultraviolet and visible spectroscopy.
Abstract: Crude ether extracts of green shoots of Cucumis sativus L. promoted the elongation of cucumber hypocotyl segments. Purification of the extract was accomplished by DEAE cellulose, silicic acid, and magnesium silicate chromatography followed by gel filtration and preparative thin layer chromatography. Identification of the growth promoter as indole-3-ethanol was achieved by mass spectrometry, thin layer and gas chromatography, and ultraviolet and visible spectroscopy, as well as by physiological characteristics.



Journal ArticleDOI
01 Jan 1967-Analyst
TL;DR: A two-dimensional thin-layer chromatographic system has been used to separate tocopherols and tocotrienols before determining them by the Emmerie-Engel reaction.
Abstract: A two-dimensional thin-layer chromatographic system has been used to separate tocopherols and tocotrienols before determining them by the Emmerie-Engel reaction. Reproducible recoveries of about 92 per cent. were obtained with authentic tocopherols and the method was used to determine tocopherols and tocotrienols in several vegetable oils. A compound, apparently plastochromanol, has been identified in some oils.

Journal ArticleDOI
TL;DR: It is postulated that the synthesis of waxes from long chain alcohols and fatty acids without activation is possible in an environment from which water is essentially excluded and can be envisioned as resulting from the interaction of two nonpolar compounds and an enzyme in a micellar state.


Journal ArticleDOI
TL;DR: By the use of thin layer chromatography, gas-liquid chromatography and the Liebermann-Burchard reaction and mass-spectrometry, 4α, 14α-dimethyl-Δ8, 24(28)-ergostadien-3β-ol (obtusifoliol) and cycloeucalenol have been demonstrated in grapefruit peel.
Abstract: By the use of thin layer chromatography, gas-liquid chromatography, the Liebermann-Burchard reaction and mass-spectrometry, 4α,14α-dimethyl-Δ8,24(28)-ergostadien-3β-ol (obtusifoliol) and cycloeucalenol have been demonstrated in grapefruit peel. Gas-liquid chromatography has also revealed the presence of these compounds in several other plant tissues.

Journal ArticleDOI
TL;DR: Thin-layer chromatography for the rapid separation of several sterols on neutral alumina impregnated with silver nitrate is described, particularly effective for sterols that differ in the number and location of olefinic bonds.

Journal ArticleDOI
TL;DR: The standard gradient described in this paper allows the detection of nucleic acid base, nucleoside and nucleotide on the same plate or sheet as well as the direct fluorometry of compounds resolved on PEI-cellulose layers.


Book ChapterDOI
TL;DR: TLC is an experimentally simple and inexpensive method that permits very rapid and efficient qualitative and even semiquantitative analysis of amino acids and amino acid mixtures and lends itself especially to the comparison of protein hydrolyzates, analysis of the course of hydrolysis experiments, characterization of peptides and their hydrolyZates, and purity control.
Abstract: Publisher Summary This chapter discusses the thin-layer chromatography (TLC) of amino acids. TLC is an experimentally simple and inexpensive method that permits very rapid and efficient qualitative and even semiquantitative analysis of amino acids and amino acid mixtures. It lends itself especially to the comparison of protein hydrolyzates, analysis of the course of hydrolysis experiments, characterization of peptides and their hydrolyzates, and purity control. The role of certain details important in the technique of TLC are layer thickness and homogeneity, degree of layer activation, solvent quality and aging of solvent mixtures, design of separation chamber and layer location, length of run of the solvent and distance from the immersion level to the origin of the chromatogram, technique of sample application and amount of applied material, and temperature and temperature control. Quantitative evaluation of chromatograms is feasible with a possible error of up to ±15% when one-dimensional separations are satisfactory and some standardization with respect to the general complexity of the mixture under investigation is possible.