Showing papers on "Thin-layer chromatography published in 1970"
TL;DR: A convenient thin-layer chromatographic screening procedure for the detection of 18 mycotoxins is described.
Abstract: A convenient thin-layer chromatographic screening procedure for the detection of 18 mycotoxins is described.
262 citations
107 citations
TL;DR: Seven metabolites were isolated by reversed phase partition chromatography and thin layer chromatography after incubation of tritium-labeled prostaglandin E2 with the soluble fraction of homogenates of guinea pig liver and showed that prostaglandsin Fα compounds can be formed from prostag landin E compounds in animal tissue.
99 citations
TL;DR: A method is described for the separation and determination of individual bile acids in serum and in intestinal contents, employing thin-layer chromatography and fluorimetry, with good results from intestinal juice and serum.
Abstract: A method is described for the separation and determination of individual bile acids in serum and in intestinal contents, employing thin-layer chromatography and fluorimetry. The mean recovery from intestinal juice was 94% and from serum 82%. Normal values for the individual bile acids of serum and examples of clinical applications of the method are given.
67 citations
56 citations
TL;DR: Prostaglandin-like activity is found in inflammatory exudate in the rat after local injection of carrageenin, and further identification of these PGs of the E-type is described.
55 citations
TL;DR: Modifications of the analytical methods are described for the rapid separation of porphyrin methyl esters bearing eight to two methyl ester groups within 10 min on small chromatocards in chloroform-ethyl acetate solvent mixtures.
Abstract: Thin-layer chromatography methods on the preparative and analytical scale are presented for the separation of porphyrin methyl esters according to the number of ester groups in each porphyrin. Complete and sharp separations are obtained on silica gel glass plates or aluminium cards run in benzene-ethyl acetate-methanol solvent systems. Dicarboxylic porphyrin esters from proto-, deuteroand hematoporphyrin IX have also been separated. Modifications of the analytical methods are described for the rapid separation of porphyrins bearing eight to two methyl ester groups within 10 min on small chromatocards in chloroform-ethyl acetate solvent mixtures. The porphyrins are determined quantitatively by spectrophotometric analysis of the eluted substances, or by fluorescence scanning of the bands of thin-layer chromatograms, using the Camag-Turner TLC-scanner. The fluorometric method is highly sensitive and permits a simultaneous relative measurement of all bands in the chromatogram. Examples of thin-layer chromatograms in combination with fluorescence scanning are presented, demonstrating the application of this new analytical method.
47 citations
TL;DR: Gradient-thickness thin-layer chromatography promises to be useful for the quantitative isolation of trace amounts not only of other types of lipids but also of classes of compounds other than lipids.
43 citations
39 citations
TL;DR: It is proposed that there are several new, undescribed neuraminic acids in the jelly coat of the eggs of the sea urchin, since the sialic acid yielded only 45% of the color in the reaction with thiobarbituric acid as compared with the direct Ehrlich assay and, moreover, no release of sIALic acid from the complex by neuraminidase treatment was obtained.
TL;DR: It is suggested that retinal-oxidizing enzyme is a metalloprotein which requires sulfhydryl groups for maximal enzymic activity.
TL;DR: The sugar phosphate esters were utilized to identify intermediates involved in the synthesis of the branched chain sugar which is present as a cell wall polysaccharide in Lemna minor and as a flavone glycoside in parsley.
TL;DR: The influence of steric hindrance has been investigated with different steroids and the utility of the MO for microassay of steroids for a doubleisotope method has been established.
TL;DR: In this article, the molecular weight distribution of oligomeric polyethylene glycol derivatives is estimated using thin-layer chromatography and photometric measurement of the spots visualized with iodine vapour.
TL;DR: Determination of molecular weight distribution of polymeric Substances by thin-layer chromatography as discussed by the authors is a well-known method for determining the molecular distribution of a polymeric substance. But it is computationally expensive.
Abstract: Determination of Molecular Weight Distribution of Polymeric Substances by Thin-Layer Chromatography
TL;DR: Frozen tissue sections were applied directly to silica gel plates, and the lipids were separated by developing the plates in different solvent systems by direct transmission densitometry.
TL;DR: Four paper and four thin-layer chromatographic systems were studied for their ability to separate colchicine from its photoisomer(s) and other congeners and the importance of identifying and quantitating the light-induced degradation products of colchichine in radioisotopic studies on the metabolic fate of this drug in the human subject was stressed.
TL;DR: The thin-layer electrophoresis has been applied to the separation of compounds in the vitamin B6 group as mentioned in this paper, which can be utilized either in conjunction with thin layer chromatography as a second dimension or independently of it in cases where thin layer does not offer sufficient separation.
Abstract: Publisher Summary The advantages of thin-layer chromatography and thin-layer electrophoresis are well documented. The thin-layer chromatography of vitamin B6 has been the subject of several studies. Thin-layer electrophoresis has been applied to the separation of compounds in the vitamin B6 group. Separation of the nonphosphorylated forms of vitamin B6 (pyridoxol, pyridoxal, pyridoxamine, and the most important metabolites, 4-pyridoxic acid and its lactone) involves problems different from those created by separation of the phosphorylated forms, and hence the two classes of compounds are discussed individually. When absorbents containing fluorescence indicators are used, all forms and derivatives of vitamin B6 can be detected through the quenching of fluorescence in ultraviolet light. Thin-layer electrophoresis adds another important possibility to separation techniques and can be utilized either in conjunction with thin-layer chromatography as a second dimension or independently of it in cases where thin-layer chromatography does not offer sufficient separation.
TL;DR: A rapid and accurate method is described for the determination of griseo-fulvin and dechlorogriseofulvin extracted from Penicillium urticae with chloroform.
Abstract: A rapid and accurate method is described for the determination of griseofulvin and dechlorogriseofulvin extracted from Penicillium urticae with chloroform. Thinlayer chromatography was used to tentatively identify griseofulvin or dechlorogriseofulvin, or both. Two gas-liquid chromatographic systems provided additional qualitative information and simultaneous quantitation of the individual compounds.
TL;DR: The steroid glucuronide, and mono- and disulphate fractions were obtained from human bile by chromatography on Sephadex LH-20 and analysed by gas-liquid chromatography and gas chromatography-mass spectrometry.
Abstract: The steroid glucuronide, and mono- and disulphate fractions were obtained from human bile by chromatography on Sephadex LH-20 The conjugates in the glucuronide fraction were hyderolysed with β-glucuronidase and the mono- and disulphate fractions were solvolysed The steroids liberated were fractionated on silicic acid and by thin layer chromatography and analysed by gas-liquid chromatography and gas chromatography-mass spectrometry The following C21O3 and C21O4 steroids were identified: 5α-pregnane-3α,17α,20α-triol, 5β-pregnane-3α,17α,20α-triol, 5-pregnene-3α,17α,20α-triol, 5-pregnene-3β,17α,20α-triol, 5α-pregnane-3α,16α,20α-triol, 3α,21-dihydroxy-5β-pregnan-20-one, 3α,11β,21-trihydroxy-5α-pregnan-20-one, 3α,11β,21-trihydroxy-5β-pregnan-20-one and 3α,21-dihydroxy-5β-pregnane-11,20-dione Concentrations of these steroids were measured in fistula or gall-bladder bile samples from five subjects
TL;DR: The specific determination of traces of nicotinamide and nicotinic acid by a combination of thin-layer chromatography and direct fluorescence spectroscopy is described and compares favourably with reflectance spectroscopic and fluorescence quenching techniques.
TL;DR: A procedure was developed that permitted several sugars to be identified in normal urine samples without prior desalting or concentration, and is readily modifed for quantification of sugars by thin-layer densitometry.
Abstract: Ten procedures for thin-layer chromatography of carbohydrates in urine were compared. The procedures differ considerably in their sensitivity and ability to separate sugars of clinical importance. A procedure was developed that permitted several sugars to be identified in normal urine samples without prior desalting or concentration. Celite was used as adsorbent, anisaldehyde as location reagent. The procedure is simple and reproducible, and is readily modifed for quantification of sugars by thin-layer densitometry.