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Showing papers on "Thin-layer chromatography published in 1973"


Journal ArticleDOI
TL;DR: A unique colorimetric method for the quantitative determination of phospholipids that does not involve the acid digestion of the lipid is described and it followed Beer's law in the range of 1-10 micro g ofospholipid phosphorus.

437 citations


Journal ArticleDOI
TL;DR: Thin-layer chromatography of PTH amino acids on polyamide sheets with a fluorescent indicator gave a 50–100-fold increase in sensitivity when compared to thin-layer polyamide plates developed by spray techniques.

324 citations


Journal ArticleDOI
TL;DR: The application of thin layer and analytical liquid chromatography to the analysis of two samples of commercial soybean lecithins gave a composition profile of the lipid classes comparable to two-dimensional thin layer chromatography, indicating the potential of this method for the complete analysis of complex lipids, such as soybeans.
Abstract: The application of thin layer and analytical liquid chromatography to the analysis of two samples of commercial soybean lecithins is described. A combination of column chromatography and quantitative thin layer chromatography showed that these products consisted of ca. 82% mixture of the major phospholipids of soybeans, phosphatidyl choline, phosphatidyl ethanolamine, and phosphatidyl inositol. The remainder of these products contained essentially the entire spectrum of lipid classes found in soybean oil-some 24 known and unknown glycolipids and phospholipids, in addition to the neutral lipids. Applications of analytical liquid chromatography to these lecithins gave a composition profile of the lipid classes comparable to two-dimensional thin layer chromatography. The potential of this method for the complete analysis of complex lipids, such as soybean lecithins, is indicated.

71 citations



Journal ArticleDOI
TL;DR: A laboratory apparatus that could be used to treat pure triglycerides under simulated deep fat frying conditions was designed and built and the volatile decomposition products produced during frying could be quantitatively collected at the same time.
Abstract: A laboratory apparatus that could be used to treat pure triglycerides under simulated deep fat frying conditions was designed and built. By the use of this apparatus, the volatile decomposition products produced during frying could be quantitatively collected at the same time. Pure trilinolein was treated in this apparatus at 185 C for 74 hr. The volatile decomposition products were collected quantitatively. Their fractionation and identification will be reported in a subsequent paper. The nonvolatile decomposition products were isolated from the treated trilinolein as the non-urea-adduct-forming methyl esters. They constituted 26.3% of the treated trilinolein and were separated into seven fractions by repeated liquid column chromatography. Chemical and physical analyses of these fractions indicated that the chemical reactions taking place under simulated deep fat frying conditions were not entirely the same as those during simple heating under air. One of the seven fractions was further purified by thin layer chromatography and then identified as a cyclic carbon to carbon linked dimer which amounted to 4.9% of the treated trilinolein. Another fraction was further purified by thin layer chromatography, followed by gas chromatography, and then identified as noncyclic hydroxy dimers formed through carbon to carbon linkages. The noncyclic dimers constituted 2.8% of the treated trilinolein. The other four fractions were depolymerized by hydroiodic acid. The depolymerization products were fractionated by thin layer chromatography and then analyzed. It was estimated that the treated trilinolein contained 8.4% of trimers formed through carbon to carbon linkages, and 4.9% of dimers and trimers joined through carbon to carbon or carbon to oxygen linkages in the same molecule and also trimers, in which all the three monomeric units were joined through carbon to oxygen linkages.

65 citations



Journal ArticleDOI
TL;DR: An adaption of two-dimensional chromatography to thin-layer cellulose is described for the separation of thymine monomers from dimers, finding a number of specific advantages over paper chromatography.

47 citations







Journal ArticleDOI
F. Kunz1
TL;DR: A one-step one-dimensional thin-layer Chromatographie method is described to separate phospholipids, i-monoglycerides, 2-Monoglyceride, cholesterol, 1,2-diglycerides, free fatty acids, triglycerides and cholesteryl esters.


Journal ArticleDOI
TL;DR: A new assay procedure using quantitative thin-layer chromatography has been developed for trimethoprim and its four major free and conjugated metabolitres based upon the measurement of the fluorescence which develops for these compounds on non-fluorescing silica gel plates.

Journal ArticleDOI
TL;DR: Glycolipids with blood group A activity were purified from hog stomach mucosa powder and the homogeneity of the purified fractions was confirmed by thin layer chromatography in neutral, acidic, and basic solvent systems.

Journal ArticleDOI
TL;DR: High pressure ion exchange chromatography has been used for separation of metabolites of doxapram from whole dog urine and the structures of the metabolites thus isolated were determined by mass spectroscopy.
Abstract: 1. High pressure ion exchange chromatography has been used for separation of metabolites of doxapram from whole dog urine. Thin layer chromatography was used for additional separation and clean-up.2. The structures of the metabolites thus isolated were determined by mass spectroscopy.3. Seven metabolites were separated and identified.

Journal ArticleDOI
TL;DR: A reversed-phase thin-layer chromatographic procedure using layers of Kieselguhr G impregnated with triolein, castor oil or paraffin oil, which is rapid, simple and relatively insensitive to many variables that have caused difficulties in adsorption TLC, such as relative humidity and the complexity of the pigment mixture.

Journal ArticleDOI
TL;DR: Affinity chromatography with CC-cellulose showed that 3~5 mg of lysozyme/ml resin was adsorbed specifically and desorbed quantitatively under mild conditions.
Abstract: As a new adsorbent of lysozyme-like enzymes, chitin coated (CC-)cellulose was prepared. CC-cellulose was stable and had good flow properties for use in column chromatography. Affinity chromatography with CC-cellulose showed that 3~5 mg of lysozyme/ml resin was adsorbed specifically and desorbed quantitatively under mild conditions. The utilities of the method of affinity chromatography with CC-cellulose are discussed.

Journal ArticleDOI
TL;DR: A simplified and relatively rapid method, based on a gas chromatographic determination preceded by a thin-layer chromatography, for the separation of heterocyclic nitrogen compounds in atmospheric particulate matter has been developed.


Journal ArticleDOI
TL;DR: Pure 1-linoleyl-2,3-distearin, which had been subjected to thermal oxidation at 200C, was separated into its polar fatty acid fraction by solvent partitioning and data obtained from combined gas chromatography-mass spectrometry indicated the presence of dimeric compounds.
Abstract: Pure 1-linoleyl-2,3-distearin, which had been subjected to thermal oxidation at 200C, was separated into its polar fatty acid fraction by solvent partitioning. Further fractionation was achieved via thin layer chromatography and partition column chromatography. These data, as well as data obtained from combined gas chromatography-mass spectrometry, indicated the presence of dimeric compounds.

Journal ArticleDOI
TL;DR: It is concluded that the rat liver mitochondria can oxidize either of the two isomeric forms of phytanic acid, the 3l,7d,11d-isomer being somewhat more rapidly oxidized, particularly at high concentrations of substrate.

Journal ArticleDOI
TL;DR: This procedure has been sucessfully applied to the analysis of skin surface lipids and is described for the detection of free fatty acids, triglycerides, wax esters and cholesterol esters on thin-layer Chromatographic plates.

Journal ArticleDOI
TL;DR: In this paper, the major ester component was isolated by preparative gas-liquid chromatography and was found to be 2,3-dihydroxyhexacosanoic acid as deduced from the mass spectra of its trimethylsilyl ether and isopropylidene derivative.

Journal ArticleDOI
TL;DR: From data collected, a two-dimensional thin-layer chromatographic system has been developed that satisfactorily resolves a mixture of the fourteen methylated bases employed in this investigation.


Journal ArticleDOI
TL;DR: In this article, the adsorption of inorganic anions on alumina thin layers developed with aqueous salts, acids and bases was studied and the effect of pH and salt concentration was discussed and compared with the tendency for the formation of ion pairs between anions and Al3+ as observed in paper electrophoresis.

Journal ArticleDOI
TL;DR: In this article, a method was developed for the separation of sixteen ergot alkaloids by thin-layer chromatography on silica gel impregnated with formamide, using solvent systems consisting of either diisopropyl ether-tetrahydrofuran-toluene-diethylamine (70:15:15):0.1) or diisophropyl e ether-to-ethanol-threetextruthanol-tohexyl-threedextrachlorine (75:20:5

Journal ArticleDOI
J.A.F. Wickramasinghe1, S.R. Shaw1
TL;DR: A convenient thin-layer chromatographic method for separating and isolating the isomeric prostaglandins A1, B1, and C1 under non-alkaline conditions was developed as a prerequisite for the in vivo investigation of the metabolism of PGA1.