Showing papers on "Thin-layer chromatography published in 1986"
TL;DR: The suitability of the present method for studies involving phospholipid synthesis was confirmed by monitoring the elimination of water-soluble compounds from the single-phase extracts using a classical phosphorus precursor, 2-[3H]glycerol-3-phosphate.
137 citations
Journal Article•
TL;DR: A high performance liquid chromatography (HPLC) method to investigate the flavonol composition of grape skins was developed using Cinsault grapes as mentioned in this paper, and the expected main grape flavonols were extracted by semi-preparative HPLC and identified by means of ultraviolet spectrophotometry, mass spectrometry, proton nuclear magnetic resonance (NMR), and thin layer chromatography analysis of the sugars released from the glycosides by acidification.
Abstract: A high performance liquid chromatography (HPLC) method to investigate the flavonol composition of grape skins was developed using Cinsault grapes. In addition to the expected main grape flavonols, several unknown compounds were separated. They were isolated by semi-preparative HPLC and identified by means of ultraviolet spectrophotometry, mass spectrometry, proton nuclear magnetic resonance (NMR), and thin layer chromatography (TLC) analysis of the sugars released from the glycosides by acidic and enzymic hydrolysis. The main flavonols were quercetin-3-glucoside, quercetin-3-glucuronide, and myricetin-3-glucoside. Minor compounds included kaempferol-3-glucoside, kaempferol-3-galactoside, and isorhamnetin-3-glucoside. The kaempferol- and myricetin-3-glucuronides and three diglycosides were also tentatively identified. Evidence was obtained that six additional pigments consisted of the chalcone forms of anthocyanins.
123 citations
TL;DR: A simple, rapid, two-dimensional TLC system is presented which resolves the four phosphoinositide cycle phospholipids as well as all commonly encountered major and minor phospholIPids.
68 citations
Journal Article•
TL;DR: Chlorodinitrobenzene, ethacrynic acid, captopril, styrene oxide, and iminocyclophosphamide were found to be substrates, each providing a different kind of electrophilic functional group for conjugation.
Abstract: Glutathione transferase activity was shown to be present in an immobilized preparation of microsomal protein. Chlorodinitrobenzene, ethacrynic acid, captopril, styrene oxide, and iminocyclophosphamide were found to be substrates, each providing a different kind of electrophilic functional group for conjugation. The glutathione conjugates were characterized by thin layer chromatography (visualized by reaction with ninhydrin) and by high pressure liquid chromatography. A variety of conditions was evaluated for analysis of these glutathiones by fast atom bombardment mass spectrometry.
60 citations
TL;DR: A simple screening method was used to investigate mycotoxin production among 114 Fusarium isolates and the importance of the use of a range of different substrates for mycot toxin production was proved.
Abstract: A simple screening method was used to investigate mycotoxin production among 114 Fusarium isolates. Zearalenone, T‐2 toxin, HT‐2 toxin, diacetoxyscirpenol, neosolaniol, deoxynivalenol, nivalenol, fusarenon‐X, butenolide, moniliformin and equisetin were detected. The importance of the use of a range of different substrates for mycotoxin production was proved.
44 citations
TL;DR: Patients with inherited adenylosuccinase deficiency excrete large quantities of succinyloaminoimidazolecarboxamide riboside (SAICAR) and succinyloadenosine (SAdo) and their concentrations are determined by cation exchange column chromatography.
44 citations
43 citations
38 citations
TL;DR: The best combination of the chromatographic systems examined for the identification of an unknown compound is G LC on OV-17, HPLC on ODS-Hypersil with acetonitrile-water as eluent and TLC using an isooctane-ethyl acetate solvent system.
37 citations
37 citations
TL;DR: Isomeric shogaols were derived by thermal dehydration of isolated gingerols of ginger (Zingiber officinale Rescoe), isolated by thin-layer chromatography and fractionated by preparative high-performance liquid chromatography as discussed by the authors.
TL;DR: A peak corresponding to a methyl octadecenoate other than oleate has been detected on the capillary gas chromatogram of the methyl esters of mango pulp fatty acids as mentioned in this paper.
Abstract: A peak corresponding to a methyl octadecenoate other than oleate has been detected on the capillary gas chromatogram of the methyl esters of mango pulp fatty acids. This octadecenoate was isolated by silica gel and argentation column chromatography, high performance liquid chromatography and argentation thin layer chromatography, and then analyzed by infrared, nuclear magnetic resonance and gas chromatography-mass spectrometry, chromatographic separations and oxidative degradation. These analytical data proved that the octadecenoic acid wascis-vaccenic acid (cis-11-octadecenoic acid). The concentration of this acid in total octadecenoic acids ranged from 35% to 50% in the pulp of mangoes from Fiji, Mexico, the Philippines and Taiwan.cis-Vaccenic acid was revealed to be one of the major component fatty acids of non-polar lipids (mainly triacylglycerols), glycolipids and phospholipids in mango pulp. The glycolipids containedcis-vaccenic acid (ca. 20%) in higher concentration than oleic acid (ca. 15%). A trace amount ofcis-vaccenic acid (0.5%) was detected in the total lipids of mango seeds. Profile of fatty acid composition of mango pulp lipids (0.2–0.3 wt% of wet pulp) was characterized by the presence of n−7 acid isomers,cis-vaccenic acid and palmitoleic acid, and unusual mono- and dienoic positional isomers.
TL;DR: In this article, a new derivatized silica gel to which a polyol is bonded is investigated as a stationary phase for high-performance liquid chromatography, and a very high percentage of modifier can be used in straight-phase chromatography on Polyol-RSiL.
TL;DR: The gingerol compounds of freeze-dried ginger (Zingiber officinale Roscoe) were extracted by liquid carbon dioxide (600-700 p.s.i.), isolated by thin-layer chromatography and fractionated into individual gingerols by preparative high-performance liquid chromatography as discussed by the authors.
TL;DR: In this paper, the methanolic extract of the cyanobacterium (blue-green alga) Spirulina geitleri has been treated with methylpheophorbides to convert all chlorophyllous pigments to their methylphephorbides.
Abstract: he methanolic extract of the cyanobacterium (blue-green alga) Spirulina geitleri has been treated with methanolic acid to convert all chlorophyllous pigments to their methylpheophorbides. Fractionation of the latter from methylpheophorbide a by thin layer chromatography and high pressure liquid chromatography yielded methylpheophorbide-RCI. Its structure has been determined as 132S-hydroxy-20-chloro-methylpheophorbide a by 1H-nuclear magnetic resonance, absorption and circular dichroism spectroscopy, mass spectrometry and by partial synthesis from chlorophyll a. The pigment is isolated from Spirulina geitleri irrespective of the use or omission of chlorinated substances during the isolation procedure.
TL;DR: The properties, apparatus and applications of overpressured thin-layer chromatography (OPTLC) have been reviewed and examples of separations of mixtures have been given.
TL;DR: In this article, the chromatographic behavior of 6-O-α-d -glucosyl, 6- O-α -maltosyl derivatives of α-, β- and γ-cyclodextrin (CD) on three kinds of high-performance liquid chromatogram (HPLC) columns and four kinds of thin-layer chromatography (TLC) plates was investigated.
TL;DR: To study the chemical transformations of these components as a function of time and environment, fingerprint residues from four males and four females were analysed by thin-layer chromatography and high performance liquid chromatography.
TL;DR: A thin layer chromatographic system including densitometry has been developed for determining cyclopiazonic acid in peanuts and corn using methanol-chloroform and derivatization with dimethylaminobenzaldehyde-HCl spray.
Abstract: A thin layer chromatographic system including densitometry has been developed for determining cyclopiazonic acid in peanuts and corn. Samples are extracted with methanol-chloroform (20 + 80); the extract is stripped of most interferences by partitioning with aqueous sodium bicarbonate followed by acidification and repartitioning with chloroform. After thin layer chromatography and derivatization with dimethylaminobenzaldehyde-HCl spray, the toxin is quantitated by reflection densitometry at 540 nm. The recovery of cyclopiazonic acid averages 90% for peanuts and 85% for corn. The absolute detection limit is 25 ng per spot which translates to a detection limit of 125 micrograms/kg for a 50 g sample.
TL;DR: In this paper, Atrazine, simazine, 2, 4-D, silvex, and chlorphenoxy acid herbicides were determined in natural water samples at 10 ppb levels by solid phase extraction on disposable C18 columns and TLC on preadsorbent silica gel layers impregnated with AgNO3, exposure to UV light, and densitometric scanning.
Abstract: Atrazine, simazine, 2, 4-D, silvex, and 2, 4, 5-T, were determined in natural water samples at 10 ppb levels by solid phase extraction on disposable C18 columns and TLC on preadsorbent silica gel layers impregnated with AgNO3, exposure to UV light, and densitometric scanning. Recoveries ranged from 70 to 88% for the triazines and 93 to 100% for chlorphenoxy acid herbicides, with average CV values of 7 to 8%. Solid phase extraction proved to be an advantageous alternative to classical liquid-liquid partition for the analysis of water for these compounds by quantitative TLC.
Journal Article•
TL;DR: Carbon-14 glucose, in which all carbon-hydrogen bonds were replaced by carbon-deuterium bonds (deuterioglucose), was extracted from algae growing in heavy water and exposed to [14C]carbon dioxide and may be a useful feature of radiopharmaceutical design.
Abstract: Carbon-14 glucose, in which all carbon-hydrogen bonds were replaced by carbon-deuterium bonds (deuterioglucose), was extracted from algae growing in heavy water and exposed to [14C]carbon dioxide. The identity of [14C]deuterioglucose was confirmed by comparison with authentic material on two high performance liquid chromatography and two thin layer chromatography systems. Fermentation to lactate followed by oxidative decarboxylation demonstrated that 35% of the 14C was on carbons 3 and 4 for deuterioglucose isolated from a 24-hr algal incubation, and 61% for a 20-min incubation. Mice were injected intravenously with either (24-hr) deuterioglucose or with 14C-labeled (protio)glucose labeled uniformly. The deuterioglucose cleared more slowly from the blood, while heart and brain accumulated label more slowly. Tissue concentrations peaked at later times for deuterioglucose. Deuteration may be a useful feature of radiopharmaceutical design.
TL;DR: The t-butyldimethylsilyl (tBuDMS) group at N-9 may be selectively hydrolyzed and the resulting mono-O-Silyl derivatives are sufficiently stable to be subjected to thin-layer chromatography and high-performance liquid chromatography.
TL;DR: The effect of solvent pH on the ion-pair reversed-phase thin-layer chromatography of four organic acids (salicylic acid, gentisic acid, 2,5- and 2,6-dihydroxybenzoic acid) has been re-investigated in this paper.
TL;DR: In this article, a diethyl ether extract from an aqueous solution of 4-methylphenol after treatment with hypochlorite was found to be mutagenic to the Ames Salmonella test strain TA100 in the absence of liver homogenate.