scispace - formally typeset
Search or ask a question
Topic

Thin-layer chromatography

About: Thin-layer chromatography is a research topic. Over the lifetime, 7494 publications have been published within this topic receiving 124179 citations. The topic is also known as: TLC.


Papers
More filters
Journal ArticleDOI
01 Oct 1981-Lipids
TL;DR: The amount of α-tocopherolquinone in rat liver has been reinvestigated comparing a conventional procedure including saponification and thin layer chromatography followed by high peformance liquid chromatography with the direct HPLC analysis of a total lipid extract.
Abstract: The amount of α-tocopherolquinone in rat liver has been reinvestigated comparing (a) a conventional procedure including saponification and thin layer chromatography followed by high peformance liquid chromatography (HPLC), with (b) the direct HPLC analysis of a total lipid extract. Recovery of added α-tocopherolquinone was quantitative with both procedures. In contrast to a recent report of 124 nmol/g in rat liver, we found no more than 1–4 nmol/g by procedure a and less than 1 nmol/g by procedure b.

44 citations

Journal ArticleDOI
TL;DR: A sulfated neolactotetraosylceramide has been isolated from the lipid extract of hog gastric mucosa and the structure of this glycolipid is proposed to be: Galbeta1 leads to 4GlcNAc(6 comes from SO3)beta1 leading to 3Galbeta1 lead to 4 Glc1 leadsto 1 ceramide.

44 citations

Journal ArticleDOI
TL;DR: In this article, a dichloromethane extract of triterpenoids from seeds of the Neem tree (Azadirachta indica) can be fractionated rapidly and with economic use of solvents using the Biotage™ flash chromatography system.
Abstract: A dichloromethane extract of the triterpenoids from seeds of the Neem tree (Azadirachta indica) can be fractionated rapidly and with economic use of solvents using the Biotage™ flash chromatography system. The fractions can be analysed by thin layer chromatography (TLC), or quantitatively, by supercritical fluid chromatography or high performance liquid chromatography (HPLC), for the determination of 11 tetranortriterpenoids, including the natural insecticide azadirachtin. After a second pass through the Biotage flash column, pure compounds, including azadirachtin and salannin can be obtained directly, although these compounds comprise less than 0.3% of the total mass of the seeds extracted and are present in a complex mixture of similar substances. Copyright © 1999 John Wiley & Sons, Ltd.

44 citations

Journal ArticleDOI
TL;DR: Not only sapogenins differing in the number and kind of polar groups, but also those differing in A/B ring junction and in configuration of hydroxyl and C-25 methyl groups have been resolved.

44 citations

Journal ArticleDOI
TL;DR: The separation of hexoseph phosphates and triosephosphates may be obtained by use of TLC, using a cellulose adsorbent without binder, which resolved into well-defined spots while 3-phosphoglyceric acid and 2-ph phosphate did not separate.

44 citations


Network Information
Related Topics (5)
High-performance liquid chromatography
47.3K papers, 1M citations
91% related
Gas chromatography
27.4K papers, 583.3K citations
89% related
Capillary electrophoresis
21.3K papers, 590.2K citations
87% related
Solid phase extraction
20.7K papers, 550.4K citations
85% related
Detection limit
34.3K papers, 644.8K citations
83% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202388
2022209
202159
202068
201990
201881