Topic
Thin-layer chromatography
About: Thin-layer chromatography is a research topic. Over the lifetime, 7494 publications have been published within this topic receiving 124179 citations. The topic is also known as: TLC.
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TL;DR: During degradation of benzo[a]pyrene, free extracellular laccase was detected with reduced activity of lignin peroxidase, manganese-dependent peroxIDase and 2,3-dioxygenase, suggesting that laccases might play an important role in the transformation of PAHs compounds.
40 citations
TL;DR: 3β-Sterols differing in unsaturation in ring B and in the side chain were separated by thin-layer chromatography and differences in resolving power between polar and nonpolar systems were observed.
40 citations
TL;DR: An high-performance liquid chromatography-thin-layer chromatography method was developed to detect the illegal use of the xenobiotic growth promotor Trenbolone acetate (TBA), and no residues of TBA or its metabolites were found in any of the 144 random samples from the Dutch public health surveillance programme.
39 citations
TL;DR: The three major fractions (II, III and V) of the five fractions of GA-like activity obtained by the thin layer chromatographic purification of an extract of hazel embryos have been examined by gas liquid chromatography and combined gas chromatography-mass spectrometry.
Abstract: The three major fractions (II, III and V) of the five fractions of gibberellin (GA)-like activity obtained by the thin layer chromatographic purification of an extract of hazel embryos have been examined by gas liquid chromatography and combined gas chromatography-mass spectrometry. By these techniques GA1 was identified as the methyl ester trimethylsilyl ether in fraction II and GA9 as the methyl ester in fraction V. Both substances were clearly separated from other components by gas liquid chromatography so that accurate determination of their concentrations was possible. GA1 accounted for most of the activity found by bioassay in fraction II while GA9 accounted for only part of the activity of fraction V. The GA-like substances in the less biologically active thin layer chromatography fractions have not yet been identified.Freshly harvested seeds contained GA1 but GA9 was not detected. After 28 days dry storage and 42 days imbibition and chilling at 5°, the GA1 content was very low and GA9 was still below the limits of detection. On subsequent exposure of the seeds to a temperature of 20° for 8 days, there was a 40-fold increase of GA1 and at least a 300-fold increase of GA9.
39 citations
TL;DR: A previously suggested close relationship between M. leprae and M. gordonae was not supported because of the homogeneity of the chain lengths of the mycolic acids in each species.
Abstract: The mycolic and fatty acids of three samples each of Mycobacterium leprae and Mycobacterium gordonae were compared. Acids released by whole-organism alkaline hydrolysis were converted to 4-nitrobenzyl esters and mycolic acids were further derivatized to t-butyldimethylsilyl ethers. Thin-layer chromatography of the derivatized long-chain extracts showed that all three M. leprae preparations contained so-called alpha-mycolates and ketomycolates but that the M. gordonae samples had a methoxymycolate in addition to the above types. Silica gel normal-phase high-performance liquid chromatography of the total mycolic acid derivatives confirmed the lack of detectable amounts of methoxymycolates in M. leprae and reverse-phase chromatography of the individual mycolate types demonstrated the homogeneity of the chain lengths of the mycolic acids in each species. Non-hydroxylated fatty acid 4-nitrobenzyl esters were transformed to methyl esters and examined by gas chromatography. Tuberculostearic (10-methyloctadecanoic) acid was a major component of the lipids of all three M. leprae preparations but it was absent in one M. gordonae strain and a very minor component in the other representatives of this latter species. On the basis of fatty and mycolic acid compositions, therefore, a previously suggested close relationship between M. leprae and M. gordonae was not supported.
39 citations