Topic
Thin-layer chromatography
About: Thin-layer chromatography is a research topic. Over the lifetime, 7494 publications have been published within this topic receiving 124179 citations. The topic is also known as: TLC.
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TL;DR: In this paper, the metal ions in each Group are separated by circular thin-layer chromatography, and several solvents and spray reagents for the Group analyses are reported; development of a chromatoplate is complete within 2 min.
38 citations
TL;DR: Crude cell membrane fractions from a number of tissues can form acidic glycolipids and Mouse mastocytoma tissue was active in forming acidic galactose lipids with UDP-galactose as substrate, and one of the products was identified as retinylphosphate Galactose.
38 citations
TL;DR: In this paper, the authors show that the insufficient resolution in discrete regions of the HPLC profile of such mixtures is caused by both intrinsic structural properties within each phospholipid class ( e.g., heterogeneity in aliphatic residues), which cause severe expansion of the eluted peaks, and by changes related to column performance.
38 citations
TL;DR: There were no statistically significant differences between HPLC and HPTLC for quantitative determination of chlorogenic acid according to the test of equality of the means.
Abstract: Two chromatographic methods, high-performance TLC (HPTLC) and HPLC, were developed and used for separation and quantitative determination of chlorogenic acid in green coffee bean extracts For HPTLC silica gel Kieselgel 60 F 254 plates with ethyl acetate/dichlormethane/formic acid/acetic acid/water (100:25:10:10:11, v/v/v/v/v) as mobile phase were used Densitometric determination of chlorogenic acid by HPTLC was performed at 330 nm A gradient RP HPLC method was carried out at 330 nm All necessary validation tests for both methods were developed for their comparison There were no statistically significant differences between HPLC and HPTLC for quantitative determination of chlorogenic acid according to the test of equality of the means
38 citations
TL;DR: Higher amounts of spermidine and spermine were found in the urines of cancer patients compared to the values of these substances in normal urine.
38 citations