Topic
Thin-layer chromatography
About: Thin-layer chromatography is a research topic. Over the lifetime, 7494 publications have been published within this topic receiving 124179 citations. The topic is also known as: TLC.
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TL;DR: In this paper, high performance displacement chromatography was used for preparative scale separation of corticosterone, deoxycorticosterone and Reichstein's substance S with high-performance liquid chromatographic (HPLC) columns and instrumentation.
36 citations
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TL;DR: Gas-liquid chromatography showed that >99% of the lipid chains derived from the substituted lipid, and that cardiolipin must be must be, are derived from yeast cytochrome oxidase.
36 citations
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TL;DR: These findings provided indirect but strong support that hypothalamic and extrahypothalamic IR-TRH was indeed TRH, and were unable to discern differences in extraHypothalamic and hypothalamic IR -TRH and synthetic TRH.
36 citations
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TL;DR: In this paper, a modified procedure for the regiospecific analysis of triacylglycerols (TAG) with a 1,3-specific lipase was described, which included supported preparations from strains of Mucor miehei andRhizopus oryzae.
Abstract: A modified procedure for the regiospecific analysis of triacylglycerols (TAG) with a 1,3-specific lipase is described. After partial lipase hydrolysis of the triacylglycerol, the released free fatty acids (FFA) and 1,2(2,3)-diacylglycerols (DAG) were isolated by thin-layer chromatography (TLC) and converted to fatty acid methyl esters (FAME). The FAME were analyzed by gas-liquid chromatography (GLC). The 1,3-specific lipases used in this study included supported preparations from strains ofMucor miehei andRhizopus oryzae. The method also was applied to the regiospecific analyses of tung nut and Chinese melon seed oil triacyglycerols, both of which contain high proportions of α-elaeostearic acid. The TAG composition of the oils was substantiated in parallel analysis of the oils by highperformance liquid chromatography with chemical ionization mass spectrometric detection of intact TAG.
36 citations
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TL;DR: In this paper, two methods of fractionation of coal tar pitch, one based on solvent solubility separation and the other on preparative thin layer chromatography were compared for effectiveness of the separation.
36 citations