Topic
Thin-layer chromatography
About: Thin-layer chromatography is a research topic. Over the lifetime, 7494 publications have been published within this topic receiving 124179 citations. The topic is also known as: TLC.
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TL;DR: In this article, high performance liquid chromatography (HPLC) in the system silica gel-dry n-hexane has been used to characterize the behaviour of several series of commercially available mixtures of chlorinated naphthalenes, viz., Halowax 1031-1051, Clonacire 90-130 and Nibren D88-D130.
35 citations
TL;DR: In this paper, different allomerization products of pheophytins have been obtained by alkaline treatment in the presence of atmospheric oxygen and isolated by normal phase thin-layer chromatography and semi-preparative high-performance liquid chromatography (HPLC).
35 citations
TL;DR: A peak corresponding to a methyl octadecenoate other than oleate has been detected on the capillary gas chromatogram of the methyl esters of mango pulp fatty acids as mentioned in this paper.
Abstract: A peak corresponding to a methyl octadecenoate other than oleate has been detected on the capillary gas chromatogram of the methyl esters of mango pulp fatty acids. This octadecenoate was isolated by silica gel and argentation column chromatography, high performance liquid chromatography and argentation thin layer chromatography, and then analyzed by infrared, nuclear magnetic resonance and gas chromatography-mass spectrometry, chromatographic separations and oxidative degradation. These analytical data proved that the octadecenoic acid wascis-vaccenic acid (cis-11-octadecenoic acid). The concentration of this acid in total octadecenoic acids ranged from 35% to 50% in the pulp of mangoes from Fiji, Mexico, the Philippines and Taiwan.cis-Vaccenic acid was revealed to be one of the major component fatty acids of non-polar lipids (mainly triacylglycerols), glycolipids and phospholipids in mango pulp. The glycolipids containedcis-vaccenic acid (ca. 20%) in higher concentration than oleic acid (ca. 15%). A trace amount ofcis-vaccenic acid (0.5%) was detected in the total lipids of mango seeds. Profile of fatty acid composition of mango pulp lipids (0.2–0.3 wt% of wet pulp) was characterized by the presence of n−7 acid isomers,cis-vaccenic acid and palmitoleic acid, and unusual mono- and dienoic positional isomers.
35 citations
35 citations
TL;DR: In this article, the electron impact mass spectrum showed the typical peaks of a chloroindolyl-3-acetic acid methyl ester: the molecular ion cluster at m/e 223, 225 and the characteristic indole fragment ions atm/e 164, 166 (M-COOCH3)+.
Abstract: Immature green peas were harvested from plants grown in water culture on Johnson's nutrient solution with radioactive chloride. Twenty five g of lyophilized peas were extracted with butanol, the extract chromatographed, first on Sephadex LH 20 and then on silica gel. The radioactive hormone was followed by scintillation counting without the use of any scintillant. The radioactive fractions were taken up in a few μl of chloroform and injected into a gas chromatograph interfaced with a mass spectrometer. Derivatization was not necessary. The electron impact mass spectrum showed the typical peaks of a chloroindolyl-3-acetic acid methyl ester: the molecular ion cluster at m/e 223, 225 and the characteristic indole fragment ions at m/e 164, 166 (M-COOCH3)+. The chlorine atom was assigned to the 4 position of the indole ring after thin layer chromatography of radioactive extract and standards of four different chlorinated indolyl-3-acetic acid methyl esters.
35 citations