Thin-layer chromatography

About: Thin-layer chromatography is a research topic. Over the lifetime, 7494 publications have been published within this topic receiving 124179 citations. The topic is also known as: TLC.

Allelopathic activity of root exudates from Leonurus sibiricus L. (Raktodrone)

Abstract: Root exudates (RE) of Leonurus sibiricus L. show the stimulatory effect of a bioassay on rice, wheat and mustard. Root exudate shows concentration-dependent activity. A stock solution (one seedling in 200 mL of 0.1% Knop’s solution for 4 days) is denoted as (1). A 10-fold higher concentration (10) was made by using a rotary vacuum evaporator; for example, 100 mL stock solution was reduced to 10 mL. For bioassay, (25), (50), (75) and (100)-fold concentrations have been used. The stimulatory effect increases with concentration (peak at 10), decreases thereafter, and at (50) (75) and (100), the effect is inhibitory (almost 100% at 75 and 100). Paper chromatography and staining suggest the presence of phenolics including caffeic acid in RE. Caffeic acid also shows the same concentration-dependent activity on rice seeds. An ether extract of acid hydrolyzed leaves shows a number of phenolics on thin layer chromatography. Among these phenolics, three are common with RE. One highly fluorescent band occurs in a much larger amount (both RE and acid hydrolyzed leaf extract), and coincides with standard caffeic acid. On ultraviolet spectrometer analysis after elution, the putative caffeic acid compound shows two characteristic peaks similar to that of caffeic acid at 277 and 231 nm, respectively. Gas liquid chromatography retention time of both standard caffeic acid and the fluorescent spot is the same.

Separation and micro-detection of oligosaccharides of glycosphingolipids by high performance cellulose thin-layer chromatography-autoradiofluorography.

Abstract: A method is described for the microscale characterization of the oligosaccharides of glycosphinglolipids using high-performance cellulose thin-layer chromatography and autoradiofluorography. With this procedure the oligosaccharides of neutral glycosphingolipids as well as gangliosides were analyzed at an average detection of 10 pmol. The method was tested with the known glycolipds from the erythrocytes of man, sheep, rabbit and guinea pig. As a further application small quantities of the as yet unidentified glycolipids of rat erythrocyte ghosts were isolated and their oligosaccharides determined.