Topic
Thin-layer chromatography
About: Thin-layer chromatography is a research topic. Over the lifetime, 7494 publications have been published within this topic receiving 124179 citations. The topic is also known as: TLC.
Papers published on a yearly basis
Papers
More filters
TL;DR: A simple and effective procedure has been developed for eliminating silica gel from gangliosides after preparative thin-layer chromatography with yields of 92% and 90% in terms of dry weight and sialic acid content.
33 citations
33 citations
TL;DR: In this article, the hexane:ethyl acetate mixture at 70:30 volume ratio was found to be an appropriate mobile phase for a normal phase chromatographic separation of free lutein.
Abstract: Marigold is a rich source of a Xanthophyll called lutein, which shows anti-oxidant and anti-cancer activities and is beneficial to eye health. This has led to various extractions and purification studies to obtain the high purity free lutein suitablefor human applications. Liquid chromatography is extensively used to purify high value natural compounds because the process results in high purity products. The suitable mobile phase and stationary phase are the key factors to achieve high purity. In this work, chromatographic separation of lutein extracted from marigold flowers was investigated, using silica gel and mixture of hexane:ethyl acetate as a stationary and a mobile phase, respectively. Initially, the suitable composition of hexane:ethyl acetate was determined using a thin layer chromatography. Hexane:ethyl acetate mixture at 70:30 volume ratio was found to be an appropriate mobile phase for a normal phase chromatographic separation of free lutein. Preliminary experiments on a semi-preparative and a preparative column carried out at the mobile phase flow rate of 10 ml/min suggested that as high as 95% purity free lutien could be obtained with a 60% approximate yield. Moreover, since the separation by chromatography arises from the adsorption of free lutein onto silica gel, thus to better understand the process, a batch adsorption study was carried out to obtain the equilibrium adsorption data. The isotherm plotted from these data was found to be reasonably described by Langmuir adsorption model.
33 citations
TL;DR: In this paper, an alternative method in which two techniques developed separately were combined: lipids saponification at low temperature and underivatized fatty acids quantification by HPLC (high performance liquid chromatography).
Abstract: The present study was undertaken to establish a simple and appropriate method for fatty acids determination where samples are not submitted to high temperatures during the preparative and quantification processes. We describe an alternative method in which two techniques developed separately were combined: lipids saponification at low temperature and underivatized fatty acids quantification by HPLC (high performance liquid chromatography). Total lipids fraction was extracted from oils and rat tissues using the Folch method. Lipids were saponified at 37°C for 2 hours and extracted with hexane. Fatty acids were separated in a reverse-phase column (C8-Shimadzu) and identified by a refractive index detector (RID-6A-Shimadzu). Fatty acid composition of oils (soybean oil, coconut fat and cocoa butter) and rat tissues (spleen and thymus) was determined. The analysis of fatty acids using this method was reproducible and the retention time, fatty acid recovery, minimal detection limit were compared to gas...
33 citations
TL;DR: The excretion of sulfatides in human urine was studied in this article, where 24-hr urine collections were filtered and the results showed that the urinary sulfatide composition was remarkably similar to that previously reported in the kidney, including differences in fatty acid pattern.
33 citations