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Thin-layer chromatography

About: Thin-layer chromatography is a research topic. Over the lifetime, 7494 publications have been published within this topic receiving 124179 citations. The topic is also known as: TLC.


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Journal ArticleDOI
TL;DR: A simple quantitative separation method for labelled norepinephrine and its metabolites by thin-layer chromatography on cellulose is described and the efficiency was tested by administration of drugs, which have known effects on the NE-metabolism.

31 citations

Journal ArticleDOI
TL;DR: An indigenous mixed bacterial culture was screened and acclimated to decolorize a sulfonated azo dye; acid red 88 (AR88) under changing growth conditions as mentioned in this paper.
Abstract: An indigenous mixed bacterial culture was screened and acclimated to decolorize a sulfonated azo dye; Acid Red 88 (AR88) under changing growth conditions. The process parameters influencing the average decolorization rate, ADRAR88 (μg min−1) was optimized using Taguchi's orthogonal array (OA-L25), under design of experiments. The value of ADRAR88 is 888.86 (μg min−1) when the operational parameters like pH, 8.0, temperature, 35°C and AR88 concentration, 500 (mg L−1) were maintained for 6 h. The mixed culture exhibited 87.89 and 90.58% reduction in the level of chemical oxygen demand (COD) and aromatic amines, respectively. It was found that increasing AR88 concentration, were found to be insignificant on the basis of analysis of variance (ANOVA) and signal-to-noise ratio (SNR) variation for the obtained responses from Taguchi's OA experiments. The decolorization by the mixed culture was confirmed by UV–vis spectrophotometry, whereas biotransformation was evidenced by spectroscopic studies like Fourier transform infrared (FTIR), proton nuclear magnetic resonance (1H-NMR), and chromatographic techniques like high performance liquid chromatography (HPLC), thin layer chromatography (TLC), and gas chromatography coupled with mass spectrometry (GC-MS) analyses. The induction and activities of various AR88 degrading oxido-reductive enzymes was elucidated. The phytotoxicity test using plant seeds revealed the extent of detoxification of parent dye compound by the mixed culture. © 2015 American Institute of Chemical Engineers Environ Prog, 34: 1455–1466, 2015

31 citations

Journal ArticleDOI
TL;DR: The major carotenoids present in water convolvulus (Ipomoea aquatica) were characterized by open-column chromatography, thin-layer chromatograhy (TLC), and high-performance chromatography (HPLC) as mentioned in this paper.

31 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202388
2022209
202159
202068
201990
201881