Topic
Thin-layer chromatography
About: Thin-layer chromatography is a research topic. Over the lifetime, 7494 publications have been published within this topic receiving 124179 citations. The topic is also known as: TLC.
Papers published on a yearly basis
Papers
More filters
TL;DR: The Salmonella mutation assay has been adapted to a number of experimental procedures used to characterize the chemical components and molecular mechanisms involved in the direct-acting mutagenicity of diesel particle extracts, and the mutagens were found to be unreactive towards purified DNA.
Abstract: The Salmonella mutation assay has been adapted to a number of experimental procedures used to characterize the chemical components and molecular mechanisms involved in the direct-acting mutagenicity of diesel particle extracts. The mutagens were found to be unreactive towards purified DNA. Mutagenic activity was decreased in nitroreductase-deficient bacteria and increased in the absence of oxygen, suggesting that bacterial enzymes active the mutagens. The extract was fractionated by thin layer chromatography using both normal and reverse phase silica gel plates. On normal silica plates, a separation was made between unsubstituted PAH, nitro-PAH and the more polar aromatic compounds. About a third of the mutagenic activity in the particle extract was recovered in fractions containing monosubstituted nitro-PAH compounds. The absorption spectra and reverse phase chromatographic properties indicate 1-nitropyrene is a predominant component but not the only mutagen in these fractions. The remaining activity was in the more polar fractions, and mutagenicity assays using anaerobic conditions and nitro-reductase-deficient bacteria suggest they contain other nitro-substituted compounds.
169 citations
TL;DR: Cab-O-Sil, a gelling agent, was used to suspend the TLC adsorbent in a liquid scintillator solution, and for this reason the procedure requires no elution steps.
167 citations
TL;DR: Escherichia coli heat-stable enterotoxin derived from a strain pathogenic for man has been purified 13,000-fold to apparent homogeneity, suggesting the presence of disulfide bridges which are required for biological activity.
167 citations
TL;DR: In this paper, the first liquid chromatographic method for the identification and quantification of seven phytosterols in olive oil was presented, using mass spectrometry detection in positive APCI (atmospheric pressure chemical ionisation) mode.
167 citations
TL;DR: The methods have been employed to study the kinetics of triolein and tricaprylin lipolysis in an in vitro lipid digestion model commonly used to assess the digestibility of novel oral lipid-based formulations.
167 citations