Topic
Thin-layer chromatography
About: Thin-layer chromatography is a research topic. Over the lifetime, 7494 publications have been published within this topic receiving 124179 citations. The topic is also known as: TLC.
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Book•
17 Apr 1965
TL;DR: Thin-Layer Chromatography edited by E. Stahl (+) is the standard laboratory reference book for applications of this analytical method in chemical, pharmaceutical and clinical analysis.
Abstract: Thin-Layer Chromatography edited by E. Stahl (+) is the standard laboratory reference book for applications of this analytical method in chemical, pharmaceutical and clinical analysis.
1,084 citations
TL;DR: Thin-Layer Chromatography as an Analytical Tool as a Preparative Tool and Qualitative Analysis for Quantitative Analysis.
Abstract: Technique
Thin-Layer Chromatography as an Analytical Tool
Qualitative Analysis
Quantitative Analysis
Thin-Layer Chromatography as a Preparative Tool
874 citations
TL;DR: With the HPLC method, polyamine titers have been reproducibly estimated for various organs of amaranth, Lemna, oat, pea, Pharbitis, and potato and the analyses correlate well with results of thin layer chromatography determinations.
Abstract: A sensitive (0.01-1 nmol) method has been developed for the analysis of polyamines in higher plant extracts based on high performance liquid chromatography (HPLC) of their benzoyl derivatives (Redmond, Tseng 1979 J Chromatogr 170: 479-481). Putrescine, cadaverine, agmatine, spermidine, spermine, and the less common polyamines nor-spermidine and homospermidine can be completely resolved by reverse phase HPLC, isocratic elution with methanol:water (64%, v/v) through a 5-mum C(18) column, and detection at 254 nm. The method can be directly applied to crude plant extracts, and it is not subject to interference by carbohydrates and phenolics. A good quantitative correlation was found between HPLC analysis of benzoylpolyamines and thin layer chromatography of their dansyl derivatives. With the HPLC method, polyamine titers have been reproducibly estimated for various organs of amaranth, Lemna, oat, pea, Pharbitis, and potato. The analyses correlate well with results of thin layer chromatography determinations.
770 citations
TL;DR: Three distinct patterns of mycobacterial mycolates were produced by two-dimensional thin-layer chromatography, good examples of each pattern being the mycolate from Mycobacterium tuberculosis, Myc Cobacterium avium and MycOBacterium fortuitum.
653 citations
TL;DR: Concentrations of the four glycosyl ceramides in plasma and in erythrocytes were determined for samples from young, healthy males and varied with the complexity of the oligosaccharide moiety and ranged from 94% with glucosyl ceramide to 71% with globoside.
642 citations