Topic
Thin-layer chromatography
About: Thin-layer chromatography is a research topic. Over the lifetime, 7494 publications have been published within this topic receiving 124179 citations. The topic is also known as: TLC.
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TL;DR: The triphytochemical screening of three extracts of Malva sylvestris L. revealed that the seed contain alkaloids, sterols and steroids, reducing sugars, tannins, emodols, starch, coumarins and anthocyanosids which give the medicines several healing properties.
Abstract: The triphytochemical screening of three extracts (etheric, ethanolic and aqueous) of Malva sylvestris L. revealed that the seed contain alkaloids, sterols and steroids, reducing sugars, tannins, emodols, starch, coumarins and the stem contain flavonoids, tannins, starch, saponins, alkaloids, emodols, sterols and steroids, reducing compounds, coumarins and anthocyanosids which give the medicines several healing properties. The separation of the bioactive compounds from the two parts of the plant extracts was carried out using thin layer chromatography (TLC). However; the aqueous acetone extract of the seed and the stem identified the phenolic compounds such as phenolic acids.
98 citations
TL;DR: This chapter discusses the analysis of hexosamines in bacterial polysaccharides by chromatographic procedures by use of colorimetry, ionophoresis, cation exchange chromatography, and paper or thin layer Chromatography, combined with staining and comparison of ninhydrin degradation products.
Abstract: Publisher Summary This chapter discusses the analysis of hexosamines in bacterial polysaccharides by chromatographic procedures. Amino sugars found to date in bacteria, with the single exception of sialic acid, include only hexose derivatives. The naturally occurring amino sugars retain aldose and amino group functions, they vary widely in properties and no single specific assay is available for the combined estimation of amino sugars. However, by use of colorimetry, ionophoresis, cation exchange chromatography, and paper or thin layer chromatography, combined with staining and comparison of ninhydrin degradation products, reasonable identifications can be made for most of the naturally occurring representatives. Cell wall mucopeptide and associated polysaccharides are prepared from both gram positive and gram negative bacterial cells. Amino sugars are detected on paper or thin layer cellulose chromatograms with (1) alkaline silver nitrate, (2) ninhydrin, (3) Elson–Morgan reagent, (4) with Morgan–Elson reagents after N -acetylation, (5) indole after deamination with nitrous acid, (6) p-anisidine HCl, (7) aniline hydrogen phthalate, and (8) silver nitrate after ninhydrin degradation. The separation of 6-deoxy-allosamine from 6-deoxy-galactosamine and 6-deoxy-gulosamine has been achieved by gas liquid chromatography of their trimethylsilyl derivatives.
98 citations
TL;DR: Thin-layer chromatographic procedures have been combined in two-dimensional techniques to give a separation of mixtures of the 2,4-dinitrophenylhydrazones of the normal homologous series of alkan-2-ones, alk- i -en-3-ones
97 citations
TL;DR: A spray reagent consisting of anisaldehyde, glacial acetic acid, and concentrated sulfuric acid is a sensitive means of detecting bile acids in thin-layer chromatography and gives characteristic colors with this reagent, thus making identification easier.
97 citations
TL;DR: The lipids from isolated human sebaceous glands have been studied by thin- layer chromatography and no thin-layer chromatographic spots were found that corresponded to the reference standards of hydrocarbon, sterol esters, free fatty acids, sterols, monoglycerides or diglycerides.
Abstract: The lipids from isolated human sebaceous glands have been studied by thin-layer chromatography. Lipids were present that corresponded to the reference standards of squalene, wax esters, and triglycerides. No thin-layer chromatographic spots were found that corresponded to the reference standards of hydrocarbon, sterol esters, free fatty acids, sterols, monoglycerides or diglycerides.
97 citations