Topic
Thin-layer chromatography
About: Thin-layer chromatography is a research topic. Over the lifetime, 7494 publications have been published within this topic receiving 124179 citations. The topic is also known as: TLC.
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96 citations
TL;DR: Thin-layer chromatographic methods are described for the separation of the following monosaccharides: glucose, galactose, glucosamine, galactsamine, N-acetylglucosamines, N -acetylgalactosamine and neuraminic acids.
96 citations
TL;DR: A rapid method for the separation and quantitation of the major lipids of tissues and lipoproteins by automated high-performance thin-layer chromatography is presented and is particularly suitable for the rapid quantification of small amounts of lipid.
96 citations
TL;DR: The prospects and the limitations of polyamines as cancer markers using chromatographic and electrophoretic techniques are examined.
96 citations
TL;DR: In this paper, the effects and interaction of PL and TOC on soybean-oil stability were evaluated using the time in days of oil samples incubated at 110C to reach a peroxide value of 100 meq/kg, and the effect of the PL was not simply a matter of pro-oxidant metal inactivation, but rather appeared to extend the effectiveness of TOC in free-radical termination.
Abstract: The phospholipids (PL), phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were purified from commercial soybean lecithin by silicic acid chromatography and preparative silica gel thin layer chromatography (TLC). Purified phosphatidylinositol (PI) was obtained commercially. Phosphatidic acid (PA) was made from PC by phospholipase D action and purified by preparative TLC. Commercial soybean tocopherols (TOC) were further purified in a florisil column. Combinations of PL and TOC were added to commercially refined, unhydrogenated soybean oil to determine the effects and interaction of PL and TOC on soybean-oil stability. Oil stability was determined by measuring the time in days of oil samples incubated at 110C to reach a peroxide value of 100 meq/kg. Additions of TOC and all PL except PA increased the stability of the oil. PI and PE appear to be more effective than PC in increasing oil stability. The effect of the PL was not simply a matter of pro-oxidant metal inactivation, but rather appeared to extend the effectiveness of the TOC in free-radical termination.
96 citations