Topic
Thin-layer chromatography
About: Thin-layer chromatography is a research topic. Over the lifetime, 7494 publications have been published within this topic receiving 124179 citations. The topic is also known as: TLC.
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TL;DR: Simple and sensitive methods for the separation and detection of biogenic amines by thin-layer chromatography have been developed and adapted for microradiometric enzyme assays with a high degree of sensitivity and specificity.
87 citations
TL;DR: The overall method, involving tissues extraction, thin-layer chromatographic separation and assay has been evaluated using pure standards and biological samples and gives good reproducibility and almost complete recovery of lipids.
87 citations
87 citations
TL;DR: In all cases tried it has been found that the papergram solvent systems and spray reagents are directly transferable to the new system, a finding which opens up the vast papergram literature to this type of thin-layer chromatography.
86 citations
Journal Article•
TL;DR: Current reports have demonstrated that improvements in separation are attainable with the use of 100 m columns, and more accurate trans fatty acid analyses can be obtained by coupling GC with either silver-nitrate thin-layer chromatography or silver- Nitrate liquid chromatography.
Abstract: trans Isomers of naturally occurring cis-unsaturated fatty acids are produced when liquid vegetable oils or marine oils are partially hydrogenated to produce margarine, shortenings, and other hardened-fat products. Isomeric trans fatty acids are also formed in the intestinal tract of ruminants, and they appear in small amounts in dairy products and ruminant meat. Currently, satisfactory analyses for the fatty acid profiles of fats containing trans fatty acids are obtained by gas chromatography (GC) using capillary columns coated with highly polar cyanosilicone stationary phases. In capillary GC methods, the key limitation has been the incomplete separation of trans-monoenoic acid isomers from their cis isomers; however, recent reports have demonstrated that improvements in separation are attainable with the use of 100 m columns. In these columns, there is very little overlap of cis and trans isomers. More accurate trans fatty acid analyses can be obtained by coupling GC with either silver-nitrate thin-layer chromatography or silver-nitrate liquid chromatography.
86 citations