Topic
Thin-layer chromatography
About: Thin-layer chromatography is a research topic. Over the lifetime, 7494 publications have been published within this topic receiving 124179 citations. The topic is also known as: TLC.
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TL;DR: The behaviour of twenty-nine steroids of the androstane series was studied by thinlayer chromatography on Silica gel G under saturated conditions and the chromatographic behaviour of the steroids in relationship to their structure was studied.
69 citations
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TL;DR: The use of silica gel partition columns has been investigated in this paper for the separation of the various gibberellins from each other, but there appears to be no report of the use of such columns for the preparation of the compounds.
Abstract: ALTHOUGH there are many chromatographic methods for separating the various gibberellins from each other, there appears to be no report of the use of silica gel partition columns. Columns are more useful for preparative separations than thin layer and paper chromatographic methods. Methods using absorption columns made with charcoal and silicic acid have been described1,2. Partition columns are usually considered to provide especially mild conditions for chromatography, which may be important with compounds that tend to be somewhat labile, such as the gibberellins.
69 citations
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TL;DR: The major metabolites of the phosphatidylinositol cycle from extracts of extracts of [(32)PO(4)]- and [(3)H]- inositol-labeled Samanea saman pulvini appear to have a variant profile.
Abstract: The major metabolites of the phosphatidylinositol cycle from extracts of [32PO4]- and [3H]-inositol-labeled Samanea saman pulvini were separated. The membrane localized phosphoinositides were separated by thin layer chromatography, identified by comparison with purified lipid standards, and quantitated based on incorporation of radiolabel. The ratio of radioactivity in phosphatidylinositol:phosphatidylinositol 4-phosphate:phosphatidylinositol 4,5-bisphosphate is about 32:8:1. The aqueous inositol phosphates were separated by anion exchange chromatography using conventional liquid chromatography and by high performance liquid chromatography (HPLC) and were identified by comparison with standards. Analysis by HPLC reveals that 32P-labeled pulvini have inositol 1-phosphate, inositol 1,4-bisphosphate, and inositol 1,4,5-trisphosphate that co-migrate with red blood cell inositol phosphates, but 3H-inositol-labeled pulvini appear to have a variant profile.
69 citations
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TL;DR: A peptide showing somatostatin-like immunoreactivity has been isolated from acid extracts of porcine upper small intestine and preliminary chemical characterization shows that it has N-terminal serine and that it is composed of som atostatin extended from its N- terminus by an additional peptide.
68 citations
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TL;DR: The purification of an angiogenic substance from the Walker 256 rat ascites tumor is reported which is mitogenic for fetal bovine aortic endothelial cells in culture and which also stimulates new blood vessel growth in vivo.
68 citations