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Thin-layer chromatography

About: Thin-layer chromatography is a research topic. Over the lifetime, 7494 publications have been published within this topic receiving 124179 citations. The topic is also known as: TLC.


Papers
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Journal ArticleDOI
TL;DR: Various techniques, which differ greatly in specificity, accuracy and simplicity, are reviewed and discussed, in terms of analytical performance, applicability to a given sample fluid, major limitations, ability to identify uncommon bile salts, including unsaturated oxo derivatives, glucuronides, sulfates, glycosides and bile alcohols.

62 citations

Journal ArticleDOI
M. Verzele1, C. Dewaele1
TL;DR: In this paper, it is shown that metal traces in high-performance liquid chromatographic bonded-phase silica gel interfere with some separations, and a test for such activity, based on the chromatography of beer bitter acids, is described.

62 citations

Journal ArticleDOI
TL;DR: The main steps of biological degradation of the drug are reduction of the keto group at C 2 oxidation at position 2′ of the isobutyl side chain selective ether cleavage at C 9 , followed by conjugation of the phenols to glucuronic acid and the same pattern of metabolite formation was observed in the rabbit, in the dog and in man.

62 citations

Journal ArticleDOI
TL;DR: In this paper, a procedure was developed for routine relative quantitative analysis of each of the ten isomeric dimethylnaphthalenes in crude oils, which requires isolation of an alkylnaphthalene fraction by thin-layer chromatography on activated alumina plates, followed by fused-silica capillary gas chromatography analysis on OV-1 and OV 1701 phases.

62 citations

Journal ArticleDOI
TL;DR: The resulting mass spectra show that only analytical quantities of approximately 1 microg of a single GSL within a complex mixture are required for the structure determination of immunostained GSLs by MS and MS/MS.
Abstract: A combined strategy of preparative high-performance thin-layer chromatography overlay assay and mass spectrometry was established for the structural characterization of immunostained glycosphingolipids (GSLs) in silica gel extracts. Crude chloroform/methanol/water (30/60/8, v/v/v) extracts of immunostained TLC bands were analyzed by nanoelectrospray low-energy CID mass spectrometry without further purification. The GSL species investigated were isomeric monosialogangliosides of the neolacto series from a ganglioside preparation of human granulocytes, the disialoganglioside GD3 from a human melanoma lipid extract, and ganglio series Gg3Cer of a neutral GSL preparation from murine lymphoreticular MDAY-D2 cells. For the specific detection of lipid-bound oligosaccharides, polyclonal chicken IgY, murine monoclonal IgG3, and IgM antibodies were used. The resulting mass spectra show that only analytical quantities of approximately 1 microg of a single GSL within a complex mixture are required for the structure determination of immunostained GSLs by MS and MS/MS. All species investigated were detected as singly charged deprotonated molecular ions, and neither buffer-derived salt adducts nor coextracted contaminants from the immunostaining procedure or the silica gel layer were observed. This effective HPTLC-MS-joined procedure offers a wide range of applications for any carbohydrate binding agents such as bacterial toxins, plant lectins, and others.

62 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
202388
2022209
202159
202068
201990
201881