Topic
Thin-layer chromatography
About: Thin-layer chromatography is a research topic. Over the lifetime, 7494 publications have been published within this topic receiving 124179 citations. The topic is also known as: TLC.
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TL;DR: Results obtained by analysis of TFA as triacylglycerols compared favourably with those obtained by using methyl esters, and the findings of the FTIR method were substantiated by applying argentation chromatography in combination with capillary gas chromatography to the same set of samples.
Abstract: Trans fatty acids (TFA) formed during biohydrogenation by ruminant animals were quantified in cows' milk fat by means of Fourier transform i.r. (FTIR) spectroscopy. When samples were analysed as the derived methyl esters, a spectral subtraction technique resulted in TFA values that were not biased by the unspecific absorption of intact triacylglycerols or influenced by the shape of the trans band. Austrian milk fat samples contained 20-50 g TFA/kg. Results obtained by analysis of TFA as triacylglycerols compared favourably with those obtained by using methyl esters. The findings of the FTIR method were further substantiated by applying argentation chromatography in combination with capillary gas chromatography to the same set of samples. Besides trans monoenoic acids, non-methylene interrupted cis, trans octadecadienoic acids contributed significantly to the total trans content. Two novel TFA, trans-11, cis-15 octadecadienoic and cis-9, trans-13 octadecadienoic acids, were identified in milk fat by means of their retention behaviour on argentation chromatography and gas chromatography as well as by an oxidative degradation procedure.
51 citations
51 citations
TL;DR: A novel delta 12-desaturase from animals, which converts oleic acid to linoleic acid, was characterized in the house cricket, Acheta domesticus, and it is reported that the oleoyl-CoA moiety used as substrate was in the form of a CoA derivative and not in the forms of a phospholipid, as it is for the plantDelta 12- Desaturase.
51 citations
51 citations
TL;DR: Surface-Enhanced Resonance Raman Spectra of Adriamycia, 11-Deoxycar minomycin, Their Model Chromophores, and Their Complexes with DNA is used to determine Purine Bases by Reversed-Phase High Performance Liquid Chromatography Using Real-Time Surface-Enhanced.
Abstract: T. W. Graham Solomms, Organic Chemistry, John Wiley & Sons 1980. Theodora W. Greene, Protective Groups In Organic Synthesis, John Wiley & Sons 1981. Giulietta Smulevich et al. \"Surface-Enhanced Reso nance Raman Spectra of Adriamycia, 11-Deoxycar minomycin, Their Model Chromophores, and Their Complexes with DNA\" Journal of Physical Chemistry, vol. 90, No. 23 (1986) 6388-6392. Therese M. Cotten et al. \"Determination of Purine Bases by Reversed-Phase High Performance Liquid Chromatography Using Real-Time Surface-Enhanced
51 citations