Toad

About: Toad is a research topic. Over the lifetime, 1624 publications have been published within this topic receiving 28732 citations.

Mechanism of action of aldosterone on active sodium transport across toad skin.

Abstract: Epithelium of the abdominal skin of the toad,Bufo marinus, has been studied by microelectrode impalement. Using an electrical equivalent circuit model, effective EMF's and specific conductances of the apical and basolateral membrane could be calculated. The skin was divided into 2 fragments for incubation in the presence, or not, of aldosterone (≥0.1 μM). After incubation overnight, sodium transport by the hormone-treated piece was increased 2.7-fold on average, compared to the untreated control. Concomitantly, conductance of the apical border increased more than 3-fold. Furthermore, mean conductance and electromotive force at the basolateral border increased by 80% and by 10%, respectively. Whether the latter changes merely represent delayed adaptation to increased apical conductance, cannot be settled from the data available.

Membrane structural and functional responses to vasopressin in toad bladder

Abstract: Freeze-fracture electronmicroscopy demonstrates that vasopressin stimulation of isolated toad bladder results in a striking morphologic alteration of epithelial membrane structure. This alteration is characterized by the aggregation of intramembranous particles in orderly linear arrays at multiple sites in the luminal membranes of granular cells specifically. The size of these aggregates varies considerably, in terms of area, over a range from 0.5 to 70×10−3 μm2. The median aggregate size is about 10.5×10−3 μm2. Since the extent of vasopressin-associated particle aggregation, in terms of frequency of sites per area of membrane or cumulative area of membrane occupied by them, closely correlates with induced changes in transport function, as measured by osmotic water flow, the aggregates themselves appear to be of physiologic significance in the mechanism of action of vasopressin. This hypothesis is supported by the observations that sites of aggregation occur (a) in response to serosal exposure to hormone specifically, (b) independently of an osmotic gradient, and (c) following stimulation with cyclic adenosine monophosphate.