Topic
Toad
About: Toad is a research topic. Over the lifetime, 1624 publications have been published within this topic receiving 28732 citations.
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5 citations
TL;DR: The inorease in weight of the prostates of immature hypophysectomised rats is unique in that it is affected neither by the follicle-stimulating hormone nor by estrogens, which is quite an accurate method, although cumbrous, time-taking, and needs a high degree of dexterity in its performance.
Abstract: THE various methods of assay of human chorionic gonadotrophin may, broadly speaking, be divided into 2 groups-first, based upon primary changes produced by the hormone in the gonads and, second, on secondary changes caused by it in the accessory reproductive organs of test animals. Assays depending on changes in the vaginal epithilium, increase in weight of uterus or of male organs, etc., belong to the laltter group and have been found to be totally unreliable and inaccurate. But the single exception of this group of tests is the inorease in weight of the prostates of immature hypophysectomised rats (Greep et dl), which is unique in that it is affected neither by the follicle-stimulating hormone nor by estrogens. I t is quite an accurate method, although cumbrous, time-taking, and needs a high degree of dexterity in its performance.
5 citations
TL;DR: Although the structure and the biological role(s) of PRL have substantially changed during evolution, the receptor for this hormone has retained many of its structural features as could be assessed between an amphibian and a mammalian species on functionally different target tissues.
Abstract: The binding characteristics of the prolactin (PRL) receptors present in toad (Bufo marinus) kidneys were investigated and compared to those of PRL receptors present in rabbit mammary glands. The molecular characteristics of the Triton X-100 solubilized renal and mammary PRL receptors were assessed by gel filtration and by migration analysis on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) after affinity labeling of the binding sites with 125I-human growth hormone. Similar results were obtained for both receptors. Partial purification of the toad PRL receptor could be achieved by affinity chromatography. The molecular weight of this purified receptor could be determined by analysis on SDS-PAGE. With the use of a polyclonal antiserum raised against a purified preparation of rabbit mammary PRL receptor, one or several antigenic epitope(s) could be identified on the core of the toad renal PRL receptor. In conclusion, although the structure and the biological role(s) of PRL have substantially changed during evolution, the receptor for this hormone has retained many of its structural features as could be assessed between an amphibian and a mammalian species on functionally different target tissues.
5 citations
TL;DR: The results demonstrated that only after they have been affected by substances released by the first 1-3 cm of the oviduct (pars recta), is the envelope sensitive to spermlysins and the oocytes fertilizable.
Abstract: In this communication I have attempted to present an overview of some contributions to the understanding of the oviduct-egg interaction in amphibians.
5 citations
Journal Article•
5 citations