scispace - formally typeset
Search or ask a question
Topic

Transcription (biology)

About: Transcription (biology) is a research topic. Over the lifetime, 56532 publications have been published within this topic receiving 2952782 citations. The topic is also known as: genetic transcription & transcription, genetic.


Papers
More filters
Journal ArticleDOI
19 Jul 1974-Nature
TL;DR: Approximately 35,000 different poly(A)-containing RNA sequences are present in HeLa cell cytoplasm and the sequences are grouped in three distinct abundance classes.
Abstract: Approximately 35,000 different poly(A)-containing RNA sequences are present in HeLa cell cytoplasm. The sequences are grouped in three distinct abundance classes.

513 citations

Journal ArticleDOI
TL;DR: The structure of different cDNA clones indicates that USF RNA is differentially spliced, and alternative exon usage may regulate the levels of functional USF protein.
Abstract: We isolated full-length cDNAs encoding the 43-kD form of human upstream stimulatory factor (USF), a cellular factor required for efficient transcription of the adenovirus major late (AdML) promoter in vitro. Sequence analysis showed USF to be a member of the c-myc-related family of DNA-binding proteins. Using proteins translated in vitro, we identified a DNA-binding domain near the carboxyl terminus, which includes both a helix-loop-helix motif and a leucine repeat. We show that USF interacts with its target DNA as a dimer. The leucine repeat is required for efficient DNA binding of the intact protein and for interactions between full-length and truncated USF proteins. Interestingly, it is not required for DNA binding of the isolated helix-loop-helix domain. The structure of different cDNA clones indicates that USF RNA is differentially spliced, and alternative exon usage may regulate the levels of functional USF protein.

511 citations

Journal ArticleDOI
TL;DR: The immunoregulatory cytokine interleukin 6 directly upregulates production of human immunodeficiency virus (HIV) in acutely as well as in chronically infected cells of monocytic lineage and synergizes with tumor necrosis factor alpha (TNF-alpha) in the induction of latent HIV expression.
Abstract: The immunoregulatory cytokine interleukin 6 (IL-6) directly upregulates production of human immunodeficiency virus (HIV) in acutely as well as in chronically infected cells of monocytic lineage. In addition, IL-6 synergizes with tumor necrosis factor alpha (TNF-alpha) in the induction of latent HIV expression. Unlike TNF-alpha, upregulation of viral expression induced by IL-6 alone does not occur at the transcriptional level and it is not associated with accumulation of HIV RNA. However, when IL-6 and TNF-alpha synergistically stimulate HIV production, accumulation of HIV RNA and increased transcription are observed, indicating that IL-6 affects HIV expression at multiple (transcriptional and post-transcriptional) levels.

511 citations

Journal ArticleDOI
TL;DR: It is reported that EGFR physically interacts with signal transducers and activators of transcription 3 (STAT3) in the nucleus, leading to transcriptional activation of inducible nitric oxide synthase (iNOS) in breast carcinomas.

511 citations

Journal ArticleDOI
TL;DR: Results demonstrate that the amino-terminal domain and the zinc finger region work in concert to confer high affinity and specific DNA- binding properties to the ROR isoforms and suggest a novel strategy to control DNA-binding activity of nuclear receptors.
Abstract: Three isoforms of a novel member of the steroid hormone nuclear receptor superfamily related to the retinoic acid receptors have been identified. The three isoforms, referred to as RORal, R0Ra2, and R0Ra3, share common DNA- and putative ligand-binding domains but are characterized by distinct amino-terminal domains generated by alternative RNA processing. An exon encoding a functionally important subregion of the amino-terminal domain of the RORa2 isoform resides on the opposite strand of a cytochrome c-processed pseudogene. Binding site selection using in vitro-synthesized proteins reveals that the RORal and R0Ra2 isoforms bind DNA as monomers to hormone response elements composed of a 6-bp AT-rich sequence preceding a half-site core motif PuGGTCA (RORE). However, RORal and RORa2 display different binding specificities: RORal binds to and constitutively activates transcription from a large subset of ROREs, whereas R0Ra2 recognizes ROREs with strict specificity and displays weaker transcriptional activity. The differential DNA-binding activity of each isoform maps to their respective amino-terminal domains. Whereas truncation of the amino-terminal domain diminishes the ability of RORal to bind DNA, a similar deletion relaxes RORa2-binding specificity to that displayed by RORal. Remarkably, transfer of the entire amino-terminal region of RORal or amino-terminal deletion of RORa2 confers RORE-binding specificities to het^erologous receptors. These results demonstrate that the amino-terminal domain and the zinc finger region work in concert to confer high affinity and specific DNA-binding properties to the ROR isoforms and suggest a novel strategy to control DNA-binding activity of nuclear receptors.

509 citations


Network Information
Related Topics (5)
RNA
111.6K papers, 5.4M citations
97% related
Regulation of gene expression
85.4K papers, 5.8M citations
96% related
Transcription factor
82.8K papers, 5.4M citations
96% related
Peptide sequence
84.1K papers, 4.3M citations
95% related
Gene
211.7K papers, 10.3M citations
94% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20229
20211,730
20201,721
20191,686
20181,571
20171,465