Showing papers on "Transcription factor published in 1981"

An adenovirus mutant defective in splicing RNA from early region 1A.

Abstract: Many primary products of transcription in eukaryotes contain sequences that are removed during maturation of mRNAs, although the structural determinants that direct this highly specific splicing reaction are unknown. Comparative sequence studies of known splice points1–4 have identified a short consensus sequence to which all known splice points conform to varying degrees. However, there is no direct evidence that this consensus sequence is necessary for splicing to occur. In this communication, an adenovirus mutant is described which contains a cis dominant defect in the splicing of RNA from one of the early transcriptional units. The defect is ascribed to changes in two adjacent nucleotides within a splice site consensus sequence. The mutations lie in a region of the adenovirus genome (the transforming region 1A) which normally produces a set of three co-terminal mRNAs differing only by the internal sequences removed during post-transcriptional processing. The mutant has thus been used not only to define sequences required for splicing, but also to investigate the pathways along which a series of related mRNAs is generated.