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Transplantation
About: Transplantation is a research topic. Over the lifetime, 276584 publications have been published within this topic receiving 7961661 citations.
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TL;DR: The newly developed transposase yields unprecedented stable gene transfer efficiencies following nonviral gene delivery that compare favorably to stable transduction efficiencies with integrating viral vectors and is expected to facilitate widespread applications in functional genomics and gene therapy.
Abstract: The Sleeping Beauty (SB) transposon is a promising technology platform for gene transfer in vertebrates; however, its efficiency of gene insertion can be a bottleneck in primary cell types. A large-scale genetic screen in mammalian cells yielded a hyperactive transposase (SB100X) with approximately 100-fold enhancement in efficiency when compared to the first-generation transposase. SB100X supported 35-50% stable gene transfer in human CD34(+) cells enriched in hematopoietic stem or progenitor cells. Transplantation of gene-marked CD34(+) cells in immunodeficient mice resulted in long-term engraftment and hematopoietic reconstitution. In addition, SB100X supported sustained (>1 year) expression of physiological levels of factor IX upon transposition in the mouse liver in vivo. Finally, SB100X reproducibly resulted in 45% stable transgenesis frequencies by pronuclear microinjection into mouse zygotes. The newly developed transposase yields unprecedented stable gene transfer efficiencies following nonviral gene delivery that compare favorably to stable transduction efficiencies with integrating viral vectors and is expected to facilitate widespread applications in functional genomics and gene therapy.
816 citations
Book•
20 Nov 2007TL;DR: Egalement disponible en version electronique par MD Consult (mot de passe requis).
Abstract: SECTION I: SURGICAL BASIC PRINCIPLES History of Surgery Molecular and Cell Biology The Role of Cytokines as Mediators of the Inflammatory Response Fluid, Electrolytes & Shock Hematologic Principles in Surgery Metabolism in Surgical Patients Wound Healing Critical Assessments of Outcomes SECTION II: PERIOPERATIVE MANAGEMENT Principles of PreopPreparation Principles of OperTechnique Surgical Infections & Choice of Antibiotics Surgical Problems in the Immunosuppressed Patient Surgical Complications Surgery in the Elderly The Surgical Treatment of Morbid Obesity Anesthesiology Principles Pain Management and Conscious Sedation Minimally Invasive Surgery SECTION III: TRAUMA & CRITICAL CARE Management of Acute Trauma Burns Bites and Stings Critical Care Respiratory Failure SECTION IV: TRANSPLANTATION AND IMMUNOLOGY Transplantation, Immunobiology & Immunosuppression Transplantation of Abdominal Organs SECTION V: SURGICAL ONCOLOGY Tumor Biology and Tumor Markers Melanoma and Cutaneous Malignancies Soft Tissue Sarcomas & Bone Tumors SECTION VI: HEAD AND NECK Head and Neck SECTION VII: BREAST Breast Breast Reconstruction SECTION VIII: ENDOCRINE The Thyroid The Parathyroid Gland Endocrine Pancreas The Pituitary and Adrenal Glands The Multiple Endocrine Neoplasia Syndromes SECTION IX: ESOPHAGUS Esophagus Hiatal Hernia and Gastroesophageal Reflux Disease SECTION X: ABDOMEN Abdominal Wall, Umbilicus, Peritonoeum, Mesenteries, Omentum & Retroperitoneum Hernias Acute Abdomen Acute Gastrointestinal Hemorrhage Stomach Small Bowel The Appendix Colon and Rectum Anus The Liver Surgical Complications of Cirrhosis & Portal Hypertension Biliary Tract Exocrine Pancreas The Spleen SECTION XI: CHEST Chest Wall and Pleura The Mediastinum Lung/Pulmonary Embolism Congenital Heart Disease Acquired Heart Disease: Coronary Insufficiency Acquired Heart Disease: Valvular SECTION XII: VASCULAR Thoracic Vasculature Cerebrovascular Disease Aneurysmal Vascular Disease Peripheral Arterial Occlusive Disease Vascular Trauma Venous Disease The Lymphatics Access and Ports SECTION XIII: SPECIALTIES IN GENERAL SURGERY Pediatric Surgery Neurosurgery Plastic Surgery Hand Surgery Emergent Care of Musculoskeletal Injuries Gynecology & The Female Reproductive Organs Urology
815 citations
TL;DR: A simple approach for large-scale transgenesis in Xenopus laevis embryos is developed and used to identify in vivo requirements for FGF signaling during gastrulation and, by contrast, embryos expressing XFD contain well-patterned nervous systems despite a putative role for F GF in neural induction.
Abstract: We have developed a simple approach for large-scale transgenesis in Xenopus laevis embryos and have used this method to identify in vivo requirements for FGF signaling during gastrulation. Plasmids are introduced into decondensed sperm nuclei in vitro using restriction enzyme-mediated integration (REMI). Transplantation of these nuclei into unfertilized eggs yields hundreds of normal, diploid embryos per day which develop to advanced stages and express integrated plasmids nonmosaically. Transgenic expression of a dominant negative mutant of the FGF receptor (XFD) after the mid-blastula stage uncouples mesoderm induction, which is normal, from maintenance of mesodermal markers, which is lost during gastrulation. By contrast, embryos expressing XFD contain well-patterned nervous systems despite a putative role for FGF in neural induction.
813 citations
Book•
01 May 1993
TL;DR: This poster presents a probabilistic procedure to characterize the response of the immune system to the presence of carbon dioxide in the airway of an invalid person.
Abstract: CONTENTS: Atmosphere Anatomy Elasticity Flow Control Pulmonary ventilation Pulmonary circulation Distribution Diffusion Carbon dioxide Oxygen Non-Respiratory functions Exercise Sleep High altitude Diving Drowning Neonates Smoking Anaesthesia Ventilatory failure Artificial ventilation Extra-Corporeal respiration Transplantation Anaemia Pulmonary oedema Col/ Atel Embolus ARDS Hypercap Hypoxia Hyperoxia
812 citations
TL;DR: Patients with drug-induced hepatocellular jaundice have 11.7% chance of progressing to death or transplantation, and amoxicillin-clavulanate stands out as the most common drug related to DILI.
811 citations