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Transposon mutagenesis

About: Transposon mutagenesis is a research topic. Over the lifetime, 2100 publications have been published within this topic receiving 117305 citations. The topic is also known as: transposition mutagenesis.


Papers
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Journal ArticleDOI
TL;DR: In this paper, a new vector strategy for the insertion of foreign genes into the genomes of gram negative bacteria not closely related to Escherichia coli was developed, which can utilize any gram negative bacterium as a recipient for conjugative DNA transfer.
Abstract: We have developed a new vector strategy for the insertion of foreign genes into the genomes of gram negative bacteria not closely related to Escherichia coli. The system consists of two components: special E. coli donor strains and derivatives of E. coli vector plasmids. The donor strains (called mobilizing strains) carry the transfer genes of the broad host range IncP–type plasmid RP4 integrated in their chromosomes. They can utilize any gram negative bacterium as a recipient for conjugative DNA transfer. The vector plasmids contain the P–type specific recognition site for mobilization (Mob site) and can be mobilized with high frequency from the donor strains. The mobilizable vectors are derived from the commonly used E. coli vectors pACYC184, pACYC177, and pBR325, and are unable to replicate in strains outside the enteric bacterial group. Therefore, they are widely applicable as transposon carrier replicons for random transposon insertion mutagenesis in any strain into which they can be mobilized but not stably maintained. The vectors are especially useful for site–directed transposon mutagenesis and for site–specific gene transfer in a wide variety of gram negative organisms.

7,278 citations

Book
01 Jan 1994
TL;DR: Methodology for General and Molecular Microbiology Morphology Light microscopy Determinative and cytological light microscopy Electron microscopy Cell fractionation Antigen-antibody reactions Growth: Physicochemical factors in growth Nutrition and media Enrichment and isolation Solid, liquid/solid and semisolid culture Liquid culture Growth measurement Culture preservation Molecular Genetics: Gene mutation Gene transfer in Gram-negative bacteria Gene transferIn Gram-positive bacteria Plasmids Transposon mutagenesis
Abstract: Methodology for General and Molecular Microbiology Morphology Light microscopy Determinative and cytological light microscopy Electron microscopy Cell fractionation Antigen-antibody reactions Growth: Physicochemical factors in growth Nutrition and media Enrichment and isolation Solid, liquid/solid and semisolid culture Liquid culture Growth measurement Culture preservation Molecular Genetics: Gene mutation Gene transfer in Gram-negative bacteria Gene transfer in Gram-positive bacteria Plasmids Transposon mutagenesis Gene cloning and expression Polymerase chain reaction Nucleic acid analysis Metabolism: Physical analysis Chemical analysis Enzymatic activity Permeability and transport Systematics: Phenotypic characterization DNA sequence similarities Ribosomal RNA hybridization and gene sequencing Nucleic acid probes General Methods: Laboratory safety Photography Records and reports

3,742 citations

Journal ArticleDOI
01 Sep 1984-Gene
TL;DR: A new method for in vitro insertional mutagenesis of genes cloned in Escherichia coli that makes use of the omega fragment, a 2.0-kb DNA segment consisting of an antibiotic resistance gene flanked by short inverted repeats carrying transcription and translation termination signals and synthetic polylinkers.

1,729 citations

Journal ArticleDOI
14 Nov 1997-Cell
TL;DR: Sleeping Beauty is an active DNA-transposon system from vertebrates for genetic transformation and insertional mutagenesis, and it mediates precise cut-and-paste transposition in fish as well as in mouse and human cells.

1,397 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023114
202276
202141
202073
201957
201852