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Trichoderma harzianum
About: Trichoderma harzianum is a research topic. Over the lifetime, 4731 publications have been published within this topic receiving 96796 citations.
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TL;DR: The results presented here demonstrate for the first time the potential application of T. harzianum CF in up-regulating the asiaticoside biogenetic pathway in C.Asiatica.
Abstract: Effect of fungal elicitors on biomass and asia- ticoside production in multiple shoot cultures of Centella asiatica was studied in a dose- and culture age- dependent manner. Addition of 3 % v/v Trichoderma harzianum culture filtrate (CF) in the growth medium on 10th day of 35 days culture cycle resulted in 9.63 mg g -1 dry weight content and 1.15 mg dry weight culture -1 yield of asiati- coside that was 2.53 and 2.35 times higher than in the un- elicited control shoots, respectively. The elicited cultures also registered 1.24 folds more biomass over the control with a growth index (GI) of 7.67, calculated as fresh weight increment over the initial inoculum weight. Elicitation of shoots with mycelial extract (ME) of the fungus Colleto- trichum lindemuthianum (1.5 % v/v, added on 0 day) on the other hand, though favored highest biomass accumu- lation amongst all the elicitation treatments (GI = 16.10) resulted in a decreased asiaticoside content of 1.10 mg g -1 dry weight that was nearly 3.5 and 8.7 times lower than in the control or T. harzianum CF-treated shoots, respectively. Treatments with mycelial extract of Fusarium oxysporum (0.5-1.5 % v/v), in general, proved inhibitory for shoot growth if added on 0 day of the culture cycle with GI = 4.85-8.45 in comparison to 11.11 in the control with a poor asiaticoside yield of only 0.18-0.42 mg dry weight culture -1 . Though the shoot biomass accumulation was marginally improved (GI = 5.68-11.94) over the untreated control when F. oxysporum ME (0.5-1.5 % v/v) was added in the medium on the 30th day of culture but the asiati- coside yield (0.18-0.94 mg dry weight culture -1 ) remained low. The results presented here demonstrate for the first time the potential application of T. harzianum CF in up- regulating the asiaticoside biogenetic pathway in C. asiatica.
40 citations
TL;DR: A series of 18-residue antibiotic-antifungal peptides, trichorzins HA and MA, were isolated from Trichoderma harzianum strains exhibiting antagonistic properties against phytopathogenic fungi.
Abstract: A series of 18-residue antibiotic-antifungal peptides, trichorzins HA and MA, were isolated from Trichoderma harzianum strains exhibiting antagonistic properties against phytopathogenic fungi. The sequences of the nine major pure peptides isolated by HPLC were determined by positive ion FAB-MS data and two-dimensional NMR measurements, including COSY, HOHAHA, ROESY and 1H-13C LRCOSY experiments.
40 citations
TL;DR: In this article, a field experiment was conducted using split-split plot design to evaluate the performance of crop rotation and fertilization on the canola in Iran using three crop rotations: chickpea, sunflower, wheat, and canola.
40 citations
TL;DR: 6PP biosynthesis was growth associated and its specific productivity showed a bell-shaped relationship with the energy dissipation rate, and T. harzianum physiology was strongly influenced by the prevailing hydrodynamic conditions as it triggered cellular metabolism and differentiation shifts.
Abstract: The influence of the agitation conditions on biomass growth, morphology, carbon metabolism, viability, and 6-pentyl-alpha-pyrone (6PP) production by Trichoderma harzianum were studied in an extractive fermentation system. Batch spore-inoculated cultures developed at dissolved oxygen concentrations above 35% of air saturation were carried out in a 14 L bioreactor. The effect of energy dissipation rate over culture performance was assessed using two sets of three Rushton turbines (having different diameters) operated at different agitation speeds. Higher mechanical stress enhanced cellular differentiation (i.e., sporulation), while yielding lower specific growth rates and increased specific CO(2) production rates (CPRs) at relatively constant specific glucose consumption rates. In addition, fungal viability and clump mean diameter decreased gradually at higher energy dissipation rates. 6PP biosynthesis was growth associated and its specific productivity showed a bell-shaped relationship with the energy dissipation rate. T. harzianum physiology was, therefore, strongly influenced by the prevailing hydrodynamic conditions as it triggered cellular metabolism and differentiation shifts.
40 citations
TL;DR: It is found that a significant increase in the level of GLU36 in the culture filtrate follows glucose exhaustion, suggesting that this enzyme is controlled by carbon catabolite repression.
Abstract: The effect of carbon sources on the level of β-1,3-glucanases in the culture filtrates of Trichoderma harzianum (Tc) was investigated. Enzyme activity was detected in all carbon sources, but highest levels were found when laminarin and purified cell walls were used. Three isoforms of β-1,3-glucanase were produced during growth of the fungus on purified cell walls. Two isoforms were produced on chitin, chitosan, N-acetylglucosamine and laminarin, while only one was detected when the fungus was grown on cellulose and glucose. A 36-kDa β-1,3-glucanase (GLU36) was secreted from T. harzianum (Tc) grown on all carbon sources tested as demonstrated by Western blot analysis. We found that a significant increase in the level of GLU36 in the culture filtrate follows glucose exhaustion, suggesting that this enzyme is controlled by carbon catabolite repression.
40 citations