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Trichoderma harzianum
About: Trichoderma harzianum is a research topic. Over the lifetime, 4731 publications have been published within this topic receiving 96796 citations.
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TL;DR: In all, 53 strains were isolated and identified at the species level by analysis of their internal transcribed spacers regions 1 and 2 of the rDNA cluster and (or) a fragment of the translation elongation factor 1 (tef1) gene, using an online interactive key for species identification in Trichoderma and ex-type strains and taxonomically established isolates of Trichodma as references.
Abstract: Trichoderma strains were sampled in 4 different bioclimatic zones of Tunisia, a Mediterranean North African country with strong climatic and edaphic variability from north to south, to assess the genetic diversity of endemic species of Trichoderma and their relationship to the bioclimatic zones. In all, 53 strains were isolated and identified at the species level by analysis of their internal transcribed spacers regions 1 and 2 (ITS1 and ITS2) of the rDNA cluster and (or) a fragment of the translation elongation factor 1 (tef1) gene, using an online interactive key for species identification in Trichoderma and ex-type strains and taxonomically established isolates of Trichoderma as references. At least 2 different species were observed in each ecosystem. Trichoderma harzianum clade VI and Trichoderma longibrachiatum were present in forest soils in north Tunisia; Trichoderma atroviride and Trichoderma hamatum were found in cultivated fields in northeast Tunisia; T. harzianum clade VI, a Trichoderma sp. close to the T. harzianum complex, and Trichoderma saturnisporum were isolated from forest soils in central Tunisia; and T. harzianum clade II and T. hamatum were present in oasis soils in south Tunisia.
38 citations
TL;DR: A close inspection of the enzymatic activity of these enzymes surprisingly revealed a marked difference between them despite the sequence similarity, with ThBgl1 has an increased tendency to catalyze transglycosylation reaction whileThBgl2 acts more as a hydrolyzing enzyme.
38 citations
TL;DR: A temperature of 30°C and an initial pH of 4.7 were found to be optimal for β-1,3-glucanase production from T. harzianum in both surface culture and submerged culture processes.
38 citations
TL;DR: The present work shows that a transformant of T. harzianum, which is silenced in the erg1 gene and accumulates high levels of squalene, also acts as an important elicitor molecule of tomato defense-related genes and induces Botrytis genes involved in BOT biosynthesis, in both cases, in a concentration-dependent manner.
Abstract: Trichoderma species are often used as biocontrol agents against plant-pathogenic fungi. A complex molecular interaction occurs among the biocontrol agent, the antagonistic fungus, and the plant. Terpenes and sterols produced by the biocontrol fungus have been found to affect gene expression in both the antagonistic fungus and the plant. The terpene trichodiene (TD) elicits the expression of genes related to tomato defense and to Botrytis virulence. We show here that TD itself is able to induce the expression of Botrytis genes involved in the synthesis of botrydial (BOT) and also induces terpene gene expression in Trichoderma spp. The terpene ergosterol, in addition to its role as a structural component of the fungal cell membranes, acts as an elicitor of defense response in plants. In the present work, using a transformant of T. harzianum, which is silenced in the erg1 gene and accumulates high levels of squalene, we show that this ergosterol precursor also acts as an important elicitor molecule of tomato defense-related genes and induces Botrytis genes involved in BOT biosynthesis, in both cases, in a concentration-dependent manner. Our data emphasize the importance of a balance of squalene and ergosterol in fungal interactions as well as in the biocontrol activity of Trichoderma spp.
38 citations
TL;DR: In this paper, the impact of trichoderma, biochar, and combination on Spinach plants under salt stress conditions was investigated, and the authors concluded that salinity is among the most significant threats hindering global food security.
Abstract: Salinity is among the most significant threats hindering global food security. The impact of Trichoderma, biochar, and combination on Spinach plants under salt stress conditions was investigated. O...
38 citations