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Trichoderma harzianum

About: Trichoderma harzianum is a research topic. Over the lifetime, 4731 publications have been published within this topic receiving 96796 citations.


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Journal ArticleDOI
TL;DR: The role of cellulase from Trichoderma harzianum strain Th22 in stimulation of 2,4-Dihydroxy-7-methoxy-2H-1, 4-benzoxazin-3(4H)-one (DIMBOA) and defense-related gene expressions in maize root against deoxynivalenol producing Fusarium graminearum (FG) by in vitro, in planta and virtual molecular modeling experiments as mentioned in this paper.

34 citations

Journal ArticleDOI
TL;DR: The results suggest the synergistic interactions between Brevibacterium halotolerans and Trichoderma harzianum for survival and improving plant growth and yield and higher population of Th may protect the subsequent crops from many phytopathogens.

34 citations

Journal ArticleDOI
TL;DR: Fungal cellulolytic and xylanolytic enzymes were assessed through a two-step process, wherein highly active cellulase- and/or hemicellulaseproducing fungi were selected in a first screening step followed by a second step to isolate the best enzymeproducer.
Abstract: Filamentous fungi colonizing rice straw were collected from 11 different sites in Korea and were identified based on characterization of their morphology and molecular properties. The fungi were divided into 25 species belonging to 16 genera, including 14 ascomycetes, one zygomycete, and one basidiomycete. Fungal cellulolytic and xylanolytic enzymes were assessed through a two-step process, wherein highly active cellulase- and/or hemicellulaseproducing fungi were selected in a first screening step followed by a second step to isolate the best enzymeproducer. Twenty-five fungal species were first screened for the production of total cellulase (TC), endo-beta-1,4 glucanase (EG), and endo-beta-1,4 xylanase (XYL) using solid-state fermentation with rice straw as substrate. From this screening, six species, namely, Aspergillus niger KUC5183, A. ochraceus KUC5204, A. versicolor KUC5201, Mucor circinelloides KUC6014, Trichoderma harzianum 1 KUC5182, and an unknown basidiomycete species, KUC8721, were selected. These six species were then incubated in liquid Mandels' media containing cellulose, glucose, rice straw, or xylan as the sole carbon source and the activities of six different enzymes were measured. Enzyme production was highly influenced by media conditions and in some cases significantly increased. Through this screening process, Trichoderma harzianum 1 KUC5182 was selected as the best enzyme producer. Rice straw and xylan were good carbon sources for the screening of cellulolytic and xylanolytic enzymes.

34 citations

Journal Article
TL;DR: Findings highlight the potential of prospecting marine- derived fungal endophytes to identify novel cell-wall degrading enzymes of value to the biofuel industry.
Abstract: Marine-derived fungi surviving as symptomless endo- phytes in seaweeds and seagrasses were screened for production of xylan-degrading enzymes. Of the eight endophyte isolates obtained from five different sea- grasses and another eight from six different marine algae, half of them exhibited xylanase activity in an agar plate assay. Further examination of these lead candidates using spectrophotometric assays revealed that Trichoderma harzianum, endophytic in the brown alga Sargassum wightii, had the maximum secreted xy- lanase and xylosidase activity. Moreover, this fungus could grow in NaCl-containing media (up to 1.2 M NaCl), and inclusion of 0.26 M NaCl in the media elic- ited a two- and three-fold increase in extracellular xylanase and xylosidase activity respectively. These findings highlight the potential of prospecting marine- derived fungal endophytes to identify novel cell-wall degrading enzymes of value to the biofuel industry.

34 citations

Journal ArticleDOI
TL;DR: The aim of this study was to isolate and to identify the pathogenic fungi accompanying interior in hard wheat seeds, and to evaluate the antagonism capability in vitro of (Trichoderma harzianum) against those pathogenic isolated fungi.
Abstract: The aim of this study was to isolate and to identify the pathogenic fungi accompanying interior in hard wheat seeds, and to evaluate the antagonism capability in vitro of (Trichoderma harzianum) against those pathogenic isolated fungi. Three samples of local hard wheat seeds (Triticum durum Desf) were brought from Oum-elbouaghi (Algeria) silos yields, follower for varieties: Vitron, Waha, and Cirta. ).They were stored for one year. The results of isolation on potato-dextroseagar (PDA) medium and after identification of fungal accompanying interior in the seeds clarified about (83) fungus isolates following for species next : Fusarium acuminatum (1 isolate) in a ratio of (1.2%), and Alternaria(82 isolates) with a ratio of (98.795 %).The Alternaria isolates apportioned among two species: Alternaria alternata with 39 isolates(12 isolates for: Alternaria alternata1 and 27 isolates for : Alternaria alternata2) and Alternaria infectoria with 43 isolates).And, besides it was clearly seen that the Cirta variety was a lowest infected with fungi(19 isolates) in ratio of (22.89 %), and both varieties Waha and Vitron with(32 isolates)for each one in a ration equal (38.554 %) . The results of the antagonistic capability in vitro of (Trichoderma harzianum) against isolated fungi, showed that: 1The direct confrontation in vitro of Trichoderma harzianum against the different fungus isolates on PDA medium, showed that: aIn the fourth day of the experiment, Trichoderma harzianum inhibited the mycelia growth of different pathogenic fungi in dual cultures, with different ratios, it was equal to: 6.66% for Fusarium acuminatum, and 23%, 26.66% and 40% for Alternaria alternata1, Alternaria alternata2, and Alternaria infectoria, respectively. b-The microscopic observations of the different interactions hyphal between Trichoderma harzianum and different fungus isolates, showed that the antagonistic fungus affected on Alternaria species with the decomposition phenomenon (Lyses), where it analyzed the mycelia and spores of Alternaria altrenata2 and Alternaria infectoria, while it analyzed the mycelia of Alternaria alternata1, compared with control. 2The remote confrontation in vitro of Trichoderma harzianum against the different fungus isolates on PDA medium showed that : aThe volatile metabolic substances of Trichoderma harzianum affected the growth of the different pathogenic fungi, with different ratios over the seven days of treatment, compared with control.bThe microscopic observations Noted that the volatile metabolic substances of Trichoderma harzianum affected some isolated fungi with mycelia analysis prevent the spore's formation of the Alternaria alternata2, while stopped the spore formation of Fusarium acuminatum, compared with control.

34 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023163
2022383
2021200
2020254
2019251
2018228