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Trichoderma harzianum
About: Trichoderma harzianum is a research topic. Over the lifetime, 4731 publications have been published within this topic receiving 96796 citations.
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TL;DR: Toxic-metabolite-emitting microbes were isolated from the indoor environment of a building where the occupant was suffering serious building-related ill-health symptoms and dissipated the mitochondrial membrane potential of boar spermatozoa.
Abstract: Toxic-metabolite-emitting microbes were isolated from the indoor environment of a building where the occupant was suffering serious building-related ill-health symptoms. Toxic substances soluble in methanol and inhibitory to spermatozoa at <10 μg (dry weight) ml−1 were found from six bacterial isolates and one fungus. The substances from isolates of Bacillus simplex and from isolates belonging to the actinobacterial genera Streptomyces and Nocardiopsis were mitochondriotoxic. These substances dissipated the mitochondrial membrane potential (Δψ) of boar spermatozoa. The substances from the Streptomyces isolates also swelled the mitochondria. The substances from isolates of Trichoderma harzianum Rifai and Bacillus pumilus damaged the cell membrane barrier function of sperm cells.
89 citations
TL;DR: The Taguchi design was applied to improve the physicochemical conditions for nano-Ag production, and the optimum conditions which increased its mass weight 3 times larger than a basal condition were as follows: AgNO3, diluted reductant and incubated at 30 °C, 200 rpm for 24 hr.
Abstract: Development of reliable and low-cost requirement for large-scale eco-friendly biogenic synthesis of metallic nanoparticles is an important step for industrial applications of bionanotechnology. In the present study, the mycosynthesis of spherical nano-Ag (12.7 ± 0.8 nm) from extracellular filtrate of local endophytic T. harzianum SYA.F4 strain which have interested mixed bioactive metabolites (alkaloids, flavonoids, tannins, phenols, nitrate reductase (320 nmol/hr/ml), carbohydrate (25 μg/μl) and total protein concentration (2.5 g/l) was reported. Industrial mycosynthesis of nano-Ag can be induced with different characters depending on the fungal cultivation and physical conditions. Taguchi design was applied to improve the physicochemical conditions for nano-Ag production, and the optimum conditions which increased its mass weight 3 times larger than a basal condition were as follows: AgNO3 (0.01 M), diluted reductant (10 v/v, pH 5) and incubated at 30 °C, 200 rpm for 24 hr. Kinetic conversion rates in submerged batch cultivation in 7 L stirred tank bioreactor on using semi-defined cultivation medium was as follows: the maximum biomass production (Xmax) and maximum nano-Ag mass weight (Pmax) calculated (60.5 g/l and 78.4 g/l respectively). The best nano-Ag concentration that formed large inhibition zones was 100 μg/ml which showed against A.alternate (43 mm) followed by Helminthosporium sp. (35 mm), Botrytis sp. (32 mm) and P. arenaria (28 mm).
89 citations
TL;DR: Extracellular xylanase production by selected isolates of the current study was characterized during their growth on xylan as a sole carbon source on solid medium and the results revealed that 93.3% of the isolates tested could degrade xylan and the highest activity against xylan was showed by members of Aspergillus, Fusarium, Penicillium and Trichoderma.
89 citations
TL;DR: Tyrosinase release was observed most frequently in interactions involving T. harzianum SIWT 25, although all other species also released tyrosin enzyme under certain conditions, and peroxidaseRelease was detected during all interspecific interactions.
89 citations
TL;DR: This study suggests the possible role of T. harzianum in the induction of antifungal compounds against F. oxysporum f.
89 citations