Topic
Trichoderma harzianum
About: Trichoderma harzianum is a research topic. Over the lifetime, 4731 publications have been published within this topic receiving 96796 citations.
Papers published on a yearly basis
Papers
More filters
82 citations
TL;DR: Using a biomimetic system based on binding of the lectin to the surface of inert nylon fibres revealed that the presence of the purified agglutinin on thesurface of the fibres specifically induced mycoparasitic behaviour in Trichoderma harzianum.
Abstract: A novel lectin was isolated and purified from the culture filtrate of the soilborne plant pathogenic fungus Sclerotium rolfsii by anion-exchange chromatography using a DEAE-Sepharose column. The lectin came through column with the flow-through, whereas all the non-agglutinating proteins present in the crude preparation remained bound to the column until elution in a NaCI gradient. SDS-PAGE analysis of the agglutinating fraction revealed single band corresponding to a protein with a molecular mass of approximately 45 kDa. Agglutination of Escherichia coli cells by the purified lectin was not inhibited by any of the mono- or disaccharides tested, wheres the glycoproteins mucin and asialomucin did inhibit agglutination. Protease as well as 1,3-β-glucanase, were found to be totally destructive to agglutination activity, indicating that both protein and 1,3-β-glucan are necessary for agglutination. Using a biomimetic system based on binding of the lectin to the surface of inert nylon fibres revealed that the presence of purified agglutinin on the surface of the fibres specifically induced mycoparasitic behaviour in Trichoderma harzianum. Trichoderma formed tightly adhering coils, which were significantly more frequent with the purified agglutinin-treated fibres than with untreated ones or with those treated with non-agglutinating extracellular proteins from S. rolfsii. Other mycoparasite-related structures, such as appressorium-like bodies and hyph loops, were only observed in the interaction between T. harzianum and the purified agglutinin-treated fibres.
81 citations
TL;DR: Four fungi capable of reducing the incidence of onion white rot relative to the untreated control in two soil-box trials are demonstrated.
Abstract: Four fungi (Chaetomium globosum, Trichoderma viride, T. harzianum, Trichoderma sp.) capable of reducing the incidence of onion white rot relative to the untreated control in two soil-box trials. When applied as a soil additive (sand: bran: fungal homogenate, 1:1:2) at the rate of 0-1% wheat bran/g dry soil, all fun gal isolates provided levels of disease control equivalent to the fungicide (procymidone 0-5 g a.i./100 g seed) treatment. The best results were achieved with the Chaetomium globosum and Trichoderma (C62) isolates which gave 78% and 73% control of white rot, respectively, in trial 1 and 67% and 73% control, respectively, in trial 2. Reduced control was observed when the test fungi were applied as seed coatings or incorporated into alginate pellets [...]
81 citations
TL;DR: Light induction ofphr1 in non-sporulating mutants shows that a complete sporulation pathway is not required for photoregulation, suggesting that photoinduction of sporulation and of photolyase expression is distinct in their photoreceptor system or in the transduction of the blue light signal.
81 citations
TL;DR: It is revealed that drenching with a T39 suspension induces systemic resistance against B. cinerea and primes salicylic acid- and ethylene-related gene expression in a manner proportional to the concentration of the biocontrol agent.
Abstract: Gray mold (Botrytis cinerea) is an important disease of tomato (Solanum lycopersicum). This study examined defense-related gene expression involved in the resistance to B. cinerea that is ...
81 citations