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Trichoderma harzianum
About: Trichoderma harzianum is a research topic. Over the lifetime, 4731 publications have been published within this topic receiving 96796 citations.
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TL;DR: It is suggested that higher levels of microbial soil biomass result in increased interactions between introduced T. harzianum and soil microorganisms, and further that microbial competition in soil favors a shift from hyphal growth to sporulation in T. HARZianum, potentially reducing its biocontrol efficacy.
73 citations
TL;DR: The purification to electrophoretical homogeneity of BGN16.1, the second beta-1, 6-glucanase enzyme, shows an endo-hydrolytic mode of action and the probable role of this enzyme in the antagonistic action of T. harzianum is discussed.
Abstract: The antagonistic fungus Trichoderma harzianum CECT 2413 produces at least two extracellular beta-1,6-glucanases, among other hydrolases acting on polysaccharides from fungal cell walls, when grown in chitin as the sole carbon source We have previously reported on the purification and biochemical characterization of the major activity, which corresponds to an acidic enzyme named BGN162 [de la Cruz, J, Pintor-Toro, JA, Benitez, T & Llobell, A (1995) J Bacteriol 177, 1864-1871] In this paper, we report on the purification to electrophoretical homogeneity of BGN161, the second beta-1, 6-glucanase enzyme BGN161 was purified by ammonium sulfate precipitation followed by adsorption and digestion of pustulan (a beta-1,6-glucan), chromatofocusing and gel-filtration chromatography BGN161 is a non-glycosylated protein with an apparent molecular mass of 51 kDa and a basic isoelectric point (pI 74-77) The enzyme was active toward substrates containing beta-1,6-glycosidic linkages, including yeast cell walls The Km was 08 mg x mL-1 with pustulan as the substrate Reaction product analysis by HPLC clearly indicated that BGN161 has an endo-hydrolytic mode of action The probable role of this enzyme in the antagonistic action of T harzianum is also discussed
72 citations
TL;DR: Pea seeds treated for 24 h with the dilution of 10 −2 of Trichoderma inoculant extracts resulted in the most rapid plant growth and highest phenolic content of pea seedlings.
72 citations
TL;DR: Recent advances in the research of Trichoderma species as potent and efficient aromatic hydrocarbon-degrading organisms are focused on as well as aimed to provide insight into its potential role in the bioremediation of soils contaminated with heavy hydrocarbons.
Abstract: Fungi belonging to Trichoderma genus are ascomycetes found in soils worldwide. Trichoderma has been studied in relation to diverse biotechnological applications and are known as successful colonizers of their common habitats. Members of this genus have been well described as effective biocontrol organisms through the production of secondary metabolites with potential applications as new antibiotics. Even though members of Trichoderma are commonly used for the commercial production of lytic enzymes, as a biological control agent, and also in the food industry, their use in xenobiotic biodegradation is limited. Trichoderma stands out as a genus with a great range of substrate utilization, a high production of antimicrobial compounds, and its ability for environmental opportunism. In this review, we focused on the recent advances in the research of Trichoderma species as potent and efficient aromatic hydrocarbon-degrading organisms, as well as aimed to provide insight into its potential role in the bioremediation of soils contaminated with heavy hydrocarbons. Several Trichoderma species are associated with the ability to metabolize a variety of both high and low molecular weight polycyclic aromatic hydrocarbons (PAHs) such as naphthalene, phenanthrene, chrysene, pyrene, and benzo[a]pyrene. PAH-degrading species include Trichoderma hamatum, Trichoderma harzianum, Trichoderma reesei, Trichoderma koningii, Trichoderma viride, Trichoderma virens, and Trichoderma asperellum using alternate enzyme systems commonly seen in other organisms, such as multicooper laccases, peroxidases, and ring-cleavage dioxygenases. Within these species, T. asperellum stands out as a versatile organism with remarkable degrading abilities, high tolerance, and a remarkable potential to be used as a remediation agent in polluted soils.
72 citations
TL;DR: The application of these formulations on chickpea and groundnut plants significantly reduced the percent mortality due to chickPEa wilt complex and groundnuts collar rot disease, respectively.
72 citations