Trichoderma harzianum

About: Trichoderma harzianum is a research topic. Over the lifetime, 4731 publications have been published within this topic receiving 96796 citations.

Identification of differentially expressed genes from Trichoderma harzianum during growth on cell wall of Fusarium solani as a tool for biotechnological application

Abstract: The species of T harzianum are well known for their biocontrol activity against many plant pathogens However, there is a lack of studies concerning its use as a biological control agent against F solani, a pathogen involved in several crop diseases In this study, we have used subtractive library hybridization (SSH) and quantitative real-time PCR (RT-qPCR) techniques in order to explore changes in T harzianum genes expression during growth on cell wall of F solani (FSCW) or glucose RT-qPCR was also used to examine the regulation of 18 genes, potentially involved in biocontrol, during confrontation between T harzianum and F solani Data obtained from two subtractive libraries were compared after annotation using the Blast2GO suite A total of 417 and 78 readable EST sequence were annotated in the FSCW and glucose libraries, respectively Functional annotation of these genes identified diverse biological processes and molecular functions required during T harzianum growth on FSCW or glucose We identified various genes of biotechnological value encoding to proteins which function such as transporters, hydrolytic activity, adherence, appressorium development and pathogenesis Fifteen genes were up-regulated and sixteen were down-regulated at least at one-time point during growth of T harzianum in FSCW During the confrontation assay most of the genes were up-regulated, mainly after contact, when the interaction has been established This study demonstrates that T harzianum expressed different genes when grown on FSCW compared to glucose It provides insights into the mechanisms of gene expression involved in mycoparasitism of T harzianum against F solani The identification and evaluation of these genes may contribute to the development of an efficient biological control agent

Application of Trichoderma harzianum SQR-T037 bio-organic fertiliser significantly controls Fusarium wilt and affects the microbial communities of continuously cropped soil of cucumber

Abstract: BACKGROUND: The reduction in diversity of the soil microbial community causes the disorder of continuous cropping. The aim of this study was to determine the effects of applying Trichoderma harzianum SQR-T037 bio-organic fertiliser (BIO) on the microbial community in continuously cropped cucumber soil. Four treatments were set: (1) control, where neither seedling nursery soil (N) nor transplanted soil (T) was amended with BIO; (2) N treatment, where nursery soil was amended with BIO (1% w/w) but transplanted soil was not; (3) N + T treatment, where BIO was added to both nursery soil (1% w/w) and transplanted soil (0.5% w/w); (4) uncropped soil, where soil was left uncropped consistently. RESULTS: A disease index of 72.2% was found for the control treatment, while the N and N + T treatments had disease indices of only 25 and 15% respectively. Analysis of the denaturing gradient gel electrophoresis (DGGE) profiles showed that the bacterial communities of the N and N + T treatments were similar to those of the uncropped soil but distinct from those of the control soil. The fungal communities of the N and N + T treatments differed from those of both the uncropped soil and the control. CONCLUSION: Addition of BIO to both the nursery soil and the transplanted soil can diversify the microbial community in continuously cropped cucumber soil and thus effectively control Fusarium wilt of cucumber plants. Copyright © 2012 Society of Chemical Industry