scispace - formally typeset
Search or ask a question
Topic

Trichoderma harzianum

About: Trichoderma harzianum is a research topic. Over the lifetime, 4731 publications have been published within this topic receiving 96796 citations.


Papers
More filters
Journal ArticleDOI
TL;DR: Treatment of banana fruits with T. viride 4 h prior to inoculation with L. theobromae provided better protection than simultaneous application or treatment 4 h after inoculation, and Trichoderma harzianum exhibited the greatest inhibition in dual culture.

61 citations

Journal ArticleDOI
TL;DR: Sugarcane bagasse was used as substrate for xylanase production by means of a strain of Trichoderma harzianum Rifai isolated from decaying Aspidosperma sp.

61 citations

Journal ArticleDOI
TL;DR: The results suggest that each β-1,3-glucanase produced by T. harzianum is different and is probably encoded by different genes.
Abstract: A β-1,3-glucanase, from culture filtrates of Trichoderma harzianum, was purified in sequential steps by gel filtration, hydrophobic interaction and ion exchange chromatography. A typical procedure provided 69-fold purification with 0.32% yield. The molecular mass of the protein was found to be approximately 29 kDa, as estimated by SDS-PAGE on a 10% slab gel. The KM and Vmax values for β-1,3-glucanase, using laminarin as substrate, were 1.72 mg ml−1 and 3.10 U ml−1, respectively. The pH optimum for the enzyme was pH 4.4 and maximum activity was obtained at 50°C. The enzyme was strongly inhibited by HgCl2 and SDS. These results suggest that each β-1,3-glucanase produced by T. harzianum is different and is probably encoded by different genes.

60 citations

Journal ArticleDOI
TL;DR: The specific mechanisms promoting increased biocontrol capacity of insect + fungus combinations, though not clearly defined here, must lie within a complex of factors including preference of R. solani as a food source for P. minuta, aversion of the insect to the two sporulating Hyphomycetes used for biOControl, and direct parasitism of P. Solani by the fungal agents.
Abstract: A rhizosphere-inhabiting collembolan, Proisotoma minuta (Insecta: Isotomidae), and three known biocontrol fungi were studied in sterilized and non-sterilized soil for suppression of Rhizoctonia solani on cotton in a greenhouse environment. R. solani in dried oat culture was incorporated into soil at four inoculum densities ranging from 10 to 150mg kg−1. Trichoderma harzianum on wheat bran and Gliocladium virens as dried oatmeal culture were incorporated at 200 and 50 mg kg−1 soil, respectively, and Laetisaria arvalis dried, micromilled mycelium was applied as a seed dressing. Each fungus was applied either alone or with a population of P. minuta at 1000 kg− soil. Most effective biological control occurred in sterilized soil when the fungal biocontrol agents were integrated with the insect population; all combinations provided more effective disease suppression than the fungal agents used alone. In non-sterilized soil, having a natural competitive microflora, only P. minuta used alone and the L. arvalis + P. minuta treatment provided consistently significant disease reduction compared to R. solaniinfested soil without added agents. Moderate disease control in non-sterilized soil was obtained with T. harzianum or G. virens when combined with the insect population. Plant-growth dry weight measurements did not consistently reflect the disease control benefit. The specific mechanisms promoting increased biocontrol capacity of insect + fungus combinations, though not clearly defined here, must lie within a complex of factors including preference of R. solani as a food source for P. minuta, aversion of the insect to the two sporulating Hyphomycetes used for biocontrol, and direct parasitism of R. solani by the fungal agents.

60 citations

Journal ArticleDOI
TL;DR: Type C-4 strain of Trichoderma harzianum was isolated as a microorganism with high cellulolytic activity and β-Glucosidase, involved in the last step of cellulose saccharification by degrading cellobiose to glucose, plays an important role in the cellulase enzyme system with a synergic action with endoglucanase and cellobio-hydrolase for cellulose degradation.
Abstract: Type C-4 strain of Trichoderma harzianum was isolated as a microorganism with high cellulolytic activity. β-Glucosidase is involved in the last step of cellulose saccharification by degrading cellobiose to glucose, and plays an important role in the cellulase enzyme system with a synergic action with endoglucanase and cellobio-hydrolase for cellulose degradation. β-Glucosidase from T. harzianum type C-4 was purified to homogeneity through Sephacryl S-300, DEAE-Sephadex A-50, and Mono P column chromatographies. It was a single polypeptide with the molecular mass of 75,000 by SDS-PAGE. The enzyme was very active at pH 5.0 and 45 °C. No significant inhibition was observed in the presence of metal ions, thiol reagents, or EDTA. The enzyme was stable in the presence of 5% ox gall and digestive enzymes. p-Nitrophenyl-β-D-cellobioside worked as a substrate for the enzyme as much as p-nitrophenyl-β-glucopyranoside. Glucose and gluconolactone showed competitive inhibition with a Ki of 1 mM and 1.8 μM, respectively...

60 citations


Network Information
Related Topics (5)
Rhizosphere
21.9K papers, 756.3K citations
82% related
Shoot
32.1K papers, 693.3K citations
82% related
Germination
51.9K papers, 877.9K citations
81% related
Sowing
33.8K papers, 273.4K citations
81% related
Oryza sativa
12.2K papers, 303.5K citations
80% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
2023163
2022383
2021200
2020254
2019251
2018228