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Trichoderma harzianum
About: Trichoderma harzianum is a research topic. Over the lifetime, 4731 publications have been published within this topic receiving 96796 citations.
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TL;DR: Strain FJ1, a filamentous fungus isolated from rotten wood, showed high ability to hydrolyze cellulosic materials and utilized various cellulosics materials and organic nitrogen sources to produce cellulases and xylanase, and also considerably a crystalline and/or insoluble material like Avicel and rice straw.
Abstract: Strain FJ1, a filamentous fungus isolated from rotten wood, showed high ability to hydrolyze cellulosic materials. To identify the strain FJ1, ITS sequencing analysis and morphological observation were performed. The strain FJ I was identified as Trichoderma harzianum. The strain produced a large amount of CMCase, xylanase, β-glucosidase, and avicelase. Optimal culture conditions for the production of the enzymes, such as pH, temperature, and inoculation concentration, were initial pH 6.0-7.0, 25-30°C, and 10 4 ea-spores/ml in Mandel's medium, respectively. T harzianum FJ I utilized various cellulosic materials and organic nitrogen sources to produce cellulases and xylanase, and also considerably a crystalline and/or insoluble material like Avicel and rice straw. The highest levels of CMCase and xylanase were 41.2 and 65.6 U/ml in 7 days of cultivation using 2.5% of carbon source (Avicel+CMC) and 0.5% of nitrogen source (peptone), respectively.
60 citations
TL;DR: It is concluded that Trichoderma and Talaromyces employing different mechanisms might be potential biocontrol agents for controlling R. solani-induced sugar beet damping-off disease.
60 citations
TL;DR: It was found that fungal growth rate on straw-based agar plates per se was indicative of the ability of white-rot fungi to establish in the presence of a competing fungus, but was a poor indicator of the fungus’ ability to degrade PAHs.
60 citations
TL;DR: The early expression profiles of these Trichoderma strains were studied after 20 h of incubation in the presence of tomato plants, using a high-density oligonucleotide (HDO) microarray, and compared to the profiles in the absence of plants as discussed by the authors.
Abstract: Trichoderma spp. are widely used as biopesticides and biofertilizers to control diseases and to promote positive physiological responses in plants. In vitro and in vivo assays with Trichoderma harzianum CECT 2413 (T34), Trichoderma virens Gv29-8 (T87) and Trichoderma hamatum IMI 224801 (T7) revealed that these strains affected the growth and development of lateral roots in tomato plants in different ways. The early expression profiles of these Trichoderma strains were studied after 20 h of incubation in the presence of tomato plants, using a high-density oligonucleotide (HDO) microarray, and compared to the profiles in the absence of plants. Out of the total 34 138 Trichoderma probe sets deposited on the microarray, 1077 (3.15 %) showed a significant change of at least 2-fold in expression in the presence of tomato plants. The numbers of probe sets identified in the individual Trichoderma strains were 593 in T. harzianum T34, 336 in T. virens T87 and 94 in T. hamatum T7. Carbohydrate metabolism – the chitin degradation enzymes N-acetylglucosamine-6-phosphate deacetylase, glucosamine-6-phosphate deaminase and chitinase – was the most significantly overrepresented process commonly observed in the three Trichoderma strains in early interactions with tomato plants. Strains T7 and T34, which had similar positive effects on plant development in biological assays, showed a significantly overrepresented hexokinase activity in interaction with tomato. In addition, genes encoding a 40S ribosomal protein and a P23 tumour protein were altered in both these strains.
59 citations
01 Jan 2010
TL;DR: Level of all the determined physiological parameters greatly changed in tomato plants in response to Fusarium oxysporum (FO), TH fungi and hormonal elicitor and reflected many components of defense signals which leading to the activation of power defense system in tomato against pathogen attack.
Abstract: Defense system of the plant against pathogen attach is the ultimate goal of any controlling process of the pathogen. Biological control and hormonal inducers represents an interesting strategy to stimulate the defense system of the plant especially when applied together. In the present work Trichoderma.Harzianum, salicylic acid and low dose of thiophanate methyl as recommended fungicide were used as a new strategy to enhance tomato defense response against wilt disease caused by Fusarium oxysporum f. sp. Lycopersici under greenhouse conditions. Changes in various physiological defenses including enzymes like polyphenol oxidase (PPO), peroxidase (POD) and acid invertase (AI); total soluble phenols; protein and chlorophyll content were investigated. In the present study, tomato plants infected by Fusarium oxysporum f. sp. Lycopersici one week after inoculated with Trichoderma Harzianum (TH) fungi (seedling root dipping and/ or soil treatment) and/ or sprayed daily for one week with hormonal inducer (SA). Plants were harvested at 35 days after pathogen infection. All applied treatments completely protected tomato seedlings against Fusarium wilt. Disease index percentage (DI%) was highly significantly reduced up to zero%. Level of all the determined physiological parameters greatly changed in tomato plants in response to Fusarium oxysporum (FO), TH fungi and hormonal elicitor and reflected many components of defense signals which leading to the activation of power defense system in tomato against pathogen attack. Application of Trichoderma harzianum and salicylic acid stimulated all these parameters not only to reach but also exceed their content in healthy control.
59 citations