Showing papers on "Trichoderma longibrachiatum published in 2017"

Mechanisms and characterization of Trichoderma longibrachiatum T6 in suppressing nematodes (Heterodera avenae) in wheat

Abstract: Heterodera avenae is an important soil-borne pathogen that affects field crops worldwide. Chemical nematicides can be used to control the nematode, but they bring toxicity to the environment and human. Trichoderma longibrachiatum has been shown to have the ability to control H. avenae cysts, but detailed microscopic observations and bioassays are lacking. In this study, we used microscopic observations and bioassays to study the effect of T. longibrachiatum T6 (TL6) on the eggs and second stage juveniles (J2s) of H. avenae, and investigate the role of TL6 in inducing the resistance to H. avenae in wheat seedling at physiological and biochemical levels. Microscopic observations recorded that TL6 parasitized on the H. avenae eggs, germinated, and produced a large number of hyphae on the eggs surface at the initial stage, thereafter, the eggs were completely surrounded by dense mycelia and the contents of eggs were lysed at the late stage. Meanwhile, the conidia suspension of TL6 parasitized on the surface of J2s, produced a large number of hyphae that penetrated the cuticle and caused deformation of the nematodes. TL6 at the concentration of 1.5×107 conidia ml-1 had the highest rates of parasitism on eggs and J2s, reflected by the highest hatching-inhibition of eggs and the mortality of J2s. In the greenhouse experiments, wheat seedlings treated with TL6 at 1.5×107 conidia ml-1 had reduced H. avenae infection, and increased plant growth significantly compared to the control. The cysts and juveniles in soil were reduced by 89.8% and 92.7%, the juveniles and females in roots were reduced by 88.3% and 91.3%, whereas the activity of chitinase and β-1, 3-glucanase, total flavonoids and lignin contents in wheat roots were increased significantly at different stage after inoculation with the eggs and TL6 conidia in comparison to the control. Maximum activity of chitinase and β-1, 3-glucanase were recorded at the 20th and 15th Days after inoculation with TL6 and thereafter it declined. The maximum contents of total flavonoids and lignin were recorded at the 35th and 40th Days after inoculation with TL6. After being stained with the rapid vital dyes of acridine orange (AO)......

Suppressive Effect of Trichoderma spp. on toxigenic Fusarium species.

Abstract: The aim of the present study was to examine the abilities of twenty-four isolates belonging to ten different Trichoderma species (i.e., Trichoderma atroviride, Trichoderma citrinoviride, Trichoderma cremeum, Trichoderma hamatum, Trichoderma harzianum, Trichoderma koningiopsis, Trichoderma longibrachiatum, Trichoderma longipile, Trichoderma viride and Trichoderma viridescens) to inhibit the mycelial growth and mycotoxin production by five Fusarium strains (i.e., Fusarium avenaceum, Fusarium cerealis, Fusarium culmorum, Fusarium graminearum and Fusarium temperatum). Dual-culture bioassay on potato dextrose agar (PDA) medium clearly documented that all of the Trichoderma strains used in the study were capable of influencing the mycelial growth of at least four of all five Fusarium species on the fourth day after co-inoculation, when there was the first apparent physical contact between antagonist and pathogen. The qualitative evaluation of the interaction between the colonies after 14 days of co-culturing on PDA medium showed that ten Trichoderma strains completely overgrew and sporulated on the colony at least one of the tested Fusarium species. Whereas, the microscopic assay provided evidence that only T. atroviride AN240 and T. viride AN255 formed dense coils around the hyphae of the pathogen from where penetration took place. Of all screened Trichoderma strains, T. atroviride AN240 was also found to be the most efficient (69-100% toxin reduction) suppressors of mycotoxins (deoxynivalenol, 3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol, nivalenol, zearalenone, beauvericin, moniliformin) production by all five Fusarium species on solid substrates. This research suggests that T. atroviride AN240 can be a promising candidate for the biological control of toxigenic Fusarium species.

Volatile organic compounds emitted by filamentous fungi isolated from flooded homes after Hurricane Sandy show toxicity in a Drosophila bioassay

Abstract: Superstorm Sandy provided an opportunity to study filamentous fungi (molds) associated with winter storm damage. We collected 36 morphologically distinct fungal isolates from flooded buildings. By combining traditional morphological and cultural characters with an analysis of ITS sequences (the fungal DNA barcode), we identified 24 fungal species that belong to eight genera: Penicillium (11 species), Fusarium (four species), Aspergillus (three species), Trichoderma (two species), and one species each of Metarhizium, Mucor, Pestalotiopsis, and Umbelopsis. Then, we used a Drosophila larval assay to assess possible toxicity of volatile organic compounds (VOCs) emitted by these molds. When cultured in a shared atmosphere with growing cultures of molds isolated after Hurricane Sandy, larval toxicity ranged from 15 to 80%. VOCs from Aspergillus niger 129B were the most toxic yielding 80% mortality to Drosophila after 12 days. The VOCs from Trichoderma longibrachiatum 117, Mucor racemosus 138a, and Metarhizium anisopliae 124 were relatively non-toxigenic. A preliminary analysis of VOCs was conducted using solid-phase microextraction-gas chromatography-mass spectrometry from two of the most toxic, two of the least toxic, and two species of intermediate toxicity. The more toxic molds produced higher concentrations of 1-octen-3-ol, 3-octanone, 3-octanol, 2-octen-1-ol, and 2-nonanone; while the less toxic molds produced more 3-methyl-1-butanol and 2-methyl-1-propanol, or an overall lower amount of volatiles. Our data support the hypothesis that at certain concentrations, some VOCs emitted by indoor molds are toxigenic.

Suspected Pulmonary Infection with Trichoderma longibrachiatum after Allogeneic Stem Cell Transplantation.

Abstract: Aspergillus and Candida species are the main causative agents of invasive fungal infections in immunocompromised human hosts. However, saprophytic fungi are now increasingly being recognized as serious pathogens. Trichoderma longibrachiatum has recently been described as an emerging pathogen in immunocompromised patients. We herein report a case of isolated suspected invasive pulmonary infection with T. longibrachiatum in a 29-year-old man with severe aplastic anemia who underwent allogeneic stem cell transplantation. A direct microscopic examination of sputum, bronchoaspiration, and bronchoalveolar lavage fluid samples revealed the presence of fungal septate hyphae. The infection was successfully treated with 1 mg/kg/day liposomal amphotericin B.

Microbial enzyme preparation for organic planting soil improvement

Abstract: The invention discloses a microbial enzyme preparation for organic planting soil improvement. The microbial enzyme preparation comprises bacillus coagulans, bacillus megaterium, bacillus mucilaginosus, bacillus subtilis, photosynthetic bacteria, Gram-positive actinomycetes, Aspergillus oryzae, Trichoderma longibrachiatum, lactic acid bacteria, filamentous yeast, Rhizopus, moss bacteria, Nocardia, Actinomyces viscosus, brown azotobacter, phosphorus dissolving bacteria, potassium dissolving bacteria and six high-quality biological enzymes such as protease, lipase, fiber hydrolase, xylanase, alpha-amylase and glucanase. After improvement with the microbial enzyme preparation for 100 days, the soil can be used as an organic cultivation standard special purpose field and can be restored to a state 50 years ago. Through combined action of microorganism and enzyme catalysis, the pesticide residues, chemical residues and various pollutants in the soil can be rapidly degraded, the crop stress resistance is improved, the yield and quality are increased, the economic efficiency is improved and the ecological environment is protected. The microbial enzyme preparation provides an important guarantee for organic planting.

Improving the activity of endoglucanase I (EGI) from Saccharomyces cerevisiae by DNA shuffling

Abstract: To enhance the specific endo-β-1,4-glucanase activity (EGI) of three mixed substances: Trichoderma sp. (Trichoderma reesei, Trichoderma longibrachiatum, and Trichoderma pseudokoningii), we optimized the efficiency of the encoding gene, eg1, using DNA shuffling and Saccharomyces cerevisiae INVSc1 as a host. One variant, SEGI8 (the high activity protein encoded by eg1 gene using DNA shuffling) was found through screening approximately 1000 variants. The extracellular enzyme work of SEGI8 reached its highest level when cultured for 96 h, at 50 °C and a pH of 5.6, akin to the wild types. Clone harboring of the best mutant SEGI8 (selected via first-round mutagenesis) produced 169–257% more activity than transformants with three wild-type EGIs. Mutant SEGI8 produced by the clone showed broad pH stability (5.6–6.6) while performing comparable thermotolerance (60–70 °C) compared to wild EGIs; it also showed a high homology of 95%, 96%, and 97%, identical to the EGI of T. reesei, T. longibrachiatum, and T. pseudokoningii, respectively. Analysis of the sequences indicated that four point mutations causing amino acid substitution (V10A/G100S/F192L/S318G) were replaced. The major structure of the active site in SEGI8 was comprised of loop regions, with the same location as β-helix in NTEGI (the protein encoded by eg1 genes in T. reesei). It has been suggested that the mutation S318G might affect substrate binding and improved actions based on an analysis of the predicted 3D structural-modeling of SEGI8. Overall, this novel system could facilitate cellulose activity and create a foundation for constructing an efficient cellulose degradation system.

Bioactive Peptaibols of Forest-Derived Trichoderma Isolates from Section Longibrachiatum

Abstract: Filamentous fungi are producers of a large number of secondary metabolites with wide spectra of biological effects. Among them, peptaibols represent a group of compounds produced mainly by members of the mycotrophic filamentous fungal genus Trichoderma. A simple peptaibol characterization strategy including purification and structural elucidation steps was applied to examine the peptaibol production of three strains from the Longibrachiatum section of genus Trichoderma, T. aethiopicum TUCIM 1817, T. novae-zelandiae TUCIM 4158 and T. pseudokoningii TUCIM 1277, all deriving from natural forest habitats (disturbed semiforest, native Notophagus forest and the bark of Beilschmiedia tawa, respectively). After the solid phase clean-up of culture extracts, mass spectrometric analysis of peptaibols produced by the examined strains was performed by on-line reversed-phase high performance liquid chromatography coupled to electrospray ionization ion trap mass spectrometry. All three examined species produced 20-residue trichobrachin-like compounds, some of which are known from the literature, while others proved to be different from any peptaibols reported so far. The spectra of the peptaibols produced by these isolates were entirely different from each other. The largest amount of peptaibols consisting of four yet unknown compounds was produced by T. pseudokoningii TUCIM 1277, while ten and eight new, trichobrachin-like compounds were detected from T. aethiopicum TUCIM 1817 and T. novae-zelandiae TUCIM 4158, respectively. Feline fetal lung cell proliferation inhibition tests and membrane damage bio-assay with boar sperm cells revealed that although T. novae-zelandiae TUCIM 4158 produced the least amount of peptaibols, its compounds were the most inhibitory to mammalian cells.

Improvement of Fungal Cellulase Production by Solid State Fermentation

Abstract: Cellulase production studies have been carried out using the fungal strains Trichoderma longibrachiatum and Aspergillus terreus by using two different lignocellulosic materials for solid state fermentation (SSF). The effect of basic fermentation parameters (substrate, moisture content and the fermentation time) on enzyme production was studied. Maximum cellulase production (FPA and endoglucanase) of Trichoderma longibrachiatum were 33.83 U/gds and 167.4 U/gds, respectively, which represented a 1.75 fold improved activities than that of Aspergillus terreus (21.5 U/gds and 95.82 U/gds, respectively) using wheat bran as substrate. The optimal conditions for cellulase production with wheat bran were found to be: initial moisture content 70% and the optimal incubation time for production was three days. The endoglucanase of Trichoderma longibrachiatum was thermostable and showed a half-life of 1 hour at 70°C, while the filter paper activity (APF) lost its total activity after 1 hour at 70°C. Results indicate the scope for further optimization of the production conditions to obtain higher cellulase titres using the Trichoderma longibrachiatum strain under SSF.

Assessment of The Performance of Chicks Fed with Wheat Bran Solid Fermented by Trichoderma longibrachiatum (SF1)

Abstract: The present study was carried out to evaluate the effect of feeding fermented wheat bran (WB) on the broiler performance. Nutritional value of wheat bran was improved through fermentation by cellulase secreting fungi isolated from soil. A total number of 90 one-day- old chicks were randomly assigned into 3 experimental groups (30 each, in 3 replicates). Group 1: diet including 0 % wheat bran (control1), Group 2: diet including 10% unfermented wheat bran (control 2), Group 3: diet including 10% wheat bran solid fermented by Trichoderma longibrachiatum (SF1). All diets were iso-caloric and iso-nitrogenous. Feed and water were provided ad libitum. The statistical evaluation of growth performance at the 5th week of age indicated significant increase in the average live body weight of birds fed with fermented wheat bran which recorded (1640 g) compared with the control-1 (1147.7g) and control-2 (1363.5 g). Feed consumption recorded significant increase to 3915.6 g/ bird, which returned to the best palatability of fermented feed. It was clearly sustained that the best significant feed conversion was recorded in group treated with fermented wheat bran (2.39), while unfermented WB group (2.41) compared to control-1(2.69). The cross sections in duodenum revealed no histopathological features related to such fungi and showing normal histological structure. Concerning the effects of feeding fermented wheat bran on some blood parameters , results showed that most levels are in normal ranges. In conclusion, wheat bran solid fermented by Trichoderma longibrachiatum (SF1) could be successfully used in poultry feed without deleterious effect.

Sugarcane filtered mud fermentation agent and sugarcane filtered mud fermentation method

Abstract: The invention discloses a sugarcane filtered mud fermentation agent and a sugarcane filtered mud fermentation method. The sugarcane filtered mud fermentation agent comprises bacillus subtilis, aspergillus oryzae, saccharomycetes and trichoderma longibrachiatum, wherein the mass ratio of the bacillus subtilis, the aspergillus oryzae, the saccharomycetes and the trichoderma longibrachiatum is (14-16):(9-11):(4-6):(4-6). According to the sugarcane filtered mud fermentation agent, a plurality of strains are combined at a proper ratio, so that the temperature is raised quickly, the high-temperature time is long, the fermentation time is short and fermentation is conducted completely.

Inoculación de Trichoderma longibrachiatum en algodón transgénico: Cambios en compuestos fenólicos y enzimas de estrés oxidativo

Abstract: espanolEl objetivo del presente estudio fue evaluar la actividad de las enzimas fenilalanina amonio-liasa (PAL), peroxidasa (POX) y la produccion de compuestos fenolicos y flavonoides en plantas de algodon transgenico en respuesta a la inoculacion con Trichoderma longibrachiatum (ICA-4). Noventa dias despues de la inoculacion con T. longibrachiatum, hojas de las plantas de algodon fueron colectadas y las enzimas PAL, POX y los compuestos fenolicos fueron determinados. Los resultados mostraron que la inoculacion aumento el contenido de compuestos fenolicos y flavonoides en 18% y 45%, respectivamente; comparadas con plantas no inoculadas. La actividad de las enzimas peroxidasa (4,88 Ux10-5/mg de proteina), y fenilalanina amonia-liasa (0,0176 U/mg de proteina) mostraron diferencias significativas en comparacion con la actividad enzimatica de plantas no inoculadas (3,48x10-5 U/mg proteina para POX y 0,01 U/mg de proteina para PAL). Estos resultados sugieren la induccion de una respuesta de resistencia sistemica inducida por la inoculacion de las semillas con la cepa Trichoderma ICA-4. EnglishThe objective of the present study was to evaluate the activity of phenylalanine ammonia-lyase (PAL), peroxidase (POX) and the production of phenolic compounds and flavonoids in transgenic cotton plants in response to inoculation with Trichoderma longibrachiatum (ICA-4). Ninety days after inoculation with T. longibrachiatum, leaves of the cotton plants were collected and the enzymes PAL, POX and phenolic compounds were determined. The results showed that inoculation increased the content of phenolic compounds and flavonoids by 18% and 45%, respectively, compared to non-inoculatedplants. The activity of the enzymes peroxidase (4.88 Ux10-5/mg protein), and phenylalanine ammonia-lyase (0.0176 U/mg protein) showed significant differences compared to the enzymatic activity of uninoculated plants (3, 48x10-5 U/mg proteinfor POX and 0.01 U/mg proteinfor PAL). These results suggest the induction of a systemic resistance response induced by seed inoculation with the Trichoderma ICA-4 strain.

Microorganism bacterium agent for preventing and treating ginger basal stem rot and preparation method and application thereof

Abstract: The invention relates to a microorganism bacterium agent for preventing and treating the ginger basal stem rot and a preparation method and application thereof. The microorganism bacterium agent comprises trichoderma longibrachiatum leavening and a mixed material; the mixed material is formed by humic acid, amino acid and medical stones; a mass ratio of the trichoderma longibrachiatum leavening to the humic acid to the amino acid to the medical stones is (3 to 6):(1 to 2):(1 to 2):(4 to 5); the number of active living bacteria in the trichoderma longibrachiatum leavening is greater than or equal to 2*10 per gram. According to the invention, the trichoderma longibrachiatum leavening is applied to prevention and treatment of the ginger basal stem rot; the microorganism bacterium agent for preventing and treating the ginger basal stem rot is prepared; the obtained number of the active living bacteria in trichoderma longibrachiatum is not smaller than 2*10 per gram; a prevention and treatment rate for the basal stem rot is high; the morbidity of the ginger basal stem rot can be greatly reduced by not less than 80%; yield of ginger can be improved by not less than 10%; the economic benefits are obvious; quality of the ginger product is improved.

Preparation method and application of Trichoderma longibrachiatum DQ2 wettable pulvis

Abstract: The invention relates to a preparation method and an application of a Trichoderma longibrachiatum DQ2 wettable pulvis. The preparation method of the Trichoderma longibrachiatum DQ2 wettable pulvis comprises the following steps: 1, inoculating a PDA slant medium with a Trichoderma longibrachiatum DQ2 strain to produce a spore liquid, inoculating a sterilized PD medium with the spore liquid, and culturing the spore liquid; 2, preparing a spore suspension used as a solid shallow tray fermentation seed liquid; 3, uniformly mixing wheat bran, diatomaceous earth, streptomycin and sterile water according to a mass ratio of 50:25:1:50 to prepare a solid shallow tray fermentation medium, packaging the solid shallow tray fermentation matrix in a plastic bag, sterilizing the packaged solid shallow tray fermentation matrix, mixing the sterilized fermentation matrix with the sold shallow tray fermentation seed liquid, carrying out culturing, and crushing an air-dried shallow tray fermentation product to obtain Trichoderma longibrachiatum DQ2 spore powder; and 4, preparing the Trichoderma longibrachiatum DQ2 wettable pulvis. The Trichoderma longibrachiatum DQ2 wettable pulvis can promote the absorption of a fertilizer and the growth of crops, is nontoxic to humans and animals, and does not pollute the environment.

Biological product for stimulating the growth of plants and their protection against phytopathogens on the basis of strains of trichoderma, strains of trichoderma for the production thereof (variants), method for producing a biological product on the basis of such strains

Abstract: FIELD: biotechnology.SUBSTANCE: group of inventions relates to biotechnology and can be used to create bioprotection of plants against phytopathogens and to stimulate their growth. Group of inventions comprises the strains of the fungus of Trichoderma longibrachiatum species (3 options); biological product for stimulating the growth of plants and their protection against phytopathogens on the basis of these strains and the method for producing a biological product. Strains of the Trichoderma longibrachiatum fungus are deposited at the ARRIAM FSBSI under registration numbers: RCAM 03324, RCAM 03323, RCAM 03325 respectively. Biological product contains spores and fragments of mycelium of above strains from 2×10to 4×10CFU/cm; has antagonistic activity against phytopathogenic fungi of the Fusarium and Cladosporium species. Method of producing a biological product involves a two-stage joint cultivation of strains in a quantitative ratio of 1:1:1 consistently on two nutrient media for 4–5 days. This group of inventions provides increase in seed germination on 4–15 % and the increase of linear sizes of plants on 0.5–24 %.EFFECT: increased seed germination and the increase of linear sizes of plants.5 cl, 2 tbl, 5 ex

Microbial inoculant and an extract of Trichoderma longibrachiatum with xylanase activity effect on chemical composition,fermentative profile and aerobic stabilityof guinea grass ( Pancium maximum Jacq.) silage

Abstract: Tropical grasses, such as guinea grass (Panicum maximum Jacq), are relatively high yielding, exhibit perennial growth, and may be conserved for periods of forage shortage. However, tropical grasses have low contents of DM and soluble carbohydrates, high buffering capacity, and can contain large air volumes trapped within the silo. These features result in prolonged plant respiration and aerobic microbial ABSTRACT. The objective of this study was to determine the effects of increasing dose of a microbial inoculant alone or in combination with a Trichoderma longibrachiatum extract with xylanase activity on total losses, chemical composition, fermentative profile, microbiological quality and aerobic stability of guinea grass (Panicum maximum Jacq. cv. Mombasa) silage. Sixty minisilos (0.022 m3) were used in a 3 × 2 factorial experiment, composed by three levels (0, 4 or 8 g · t−1 of fresh forage) of microbial inoculant (INO) and two levels (0 or 1 IU · g−1 of fresh forage) of enzyme product (ENZ). INO consisted of Lactobacillus plantarum at 4 × 1010 cfu · g−1 and Pediococcus acidilactici at 4 × 1010 cfu · g−1. Silos were opened after 60 days. The combination of INO8 with ENZ caused the lowest gas losses. ENZ increased silage crude protein content, as well as the dry matter and neutral detergent fibre (NDF) in vitro digestibility. INO doses exerted a positive quadratic effect on NDF in vitro digestibility. ENZ addition increased acetic acid concentration, while INO treatments linearly decreased acetic and butyric acid concentrations and linearly increased lactic and propionic acid concentrations in silage. INO exhibited a negative quadratic effect on pH and NH3-N concentration of guinea grass silage and positive linear increase in the counts of anaerobic bacteria. Combinations of ENZ and INO8 decreased silage aerobic stability. Although there was observed no combined effect of ENZ and INO on silage chemical composition and fermentative profile, they exerted positive influence on NDF in vitro digestibility of the guinea grass silage when added alone (ENZ and INO at a dose of 4 g · t−1). Received: 22 April 2017 Revised: 1 August 2017 Accepted: 4 December 2017

Screening of strains of soil micromycetes – antagonists of fungal and bacterial plant pathogens

Abstract: The antagonistic activity of 23 strains of micromycetes belonging to different taxonomic groups, against phythopathogenic bacteria and fungi was studied. The antagonistic activity of the micromycetes was tested by agar diffusion (the method of blocks). For the determination of the influence of the micromycetes on plants, spring barley seeds were treated by cultural liquid of fungi (dilution 1 : 10) for 24 hours and germinated in Petri dishes on moist filter paper. Two strains Trichoderma longibrachiatum 17 and T. lignorum 14 showed the highest antagonistic activity against the phytopathogenic bacteria and fungi. T. longibrachiatum 17 actively suppressed the growth of fungi Fusarium oxysporum 54201, F . culmorum 50716, F . oxysporum 12, F . moniliforme 23, Cladosporium herbarum 16878, Alternaria alternata 16, Aspergillus niger 25 and bacteria Agrobacterium tumefaciens 8628, Xanthomonas campestris 8003b, Pectobacterium carotovorum 8982, Pseudomonas syringae pv. atrofaciens 8254, P. syringae pv. lachrymans 7595, zones inhibition of growth were 20.7–38.3 and 14.7–24.7 mm, respectively. The strain of T. lignorum 14 inhibited the growth of fungi F. culmorum 50716, C . herbarum 16878, F . moniliforme 23, A . alternata 16, A . niger 25 and bacteria A. tumefaciens 8628, P. carotovorum 8982, P. syringae pv. atrofaciens 8254, P. syringae pv. lachrymans 7595, zones of inhibition of growth were 14.0–38.7 and 12.3–23.3 mm, respectively. Treatment of spring barley seeds by T. longibrachiatum 17 cultural liquid showed a positive effect on seed germination, both strains T. longibrachiatum 17 and T. lignorum 14 increased the dry weight of the roots (by 17.5% and 22.0%, respectively) and the stems (by 8.0%) of spring barley plants compared with the water-treated controls. The results presented in this article indicate that the strains T. longibrachiatum 17 and T. lignorum 14 can be recommended as promising microbial agents to protect plants from fungal and bacterial diseases.

Preparation method of an enzyme detergent for wooden furniture

Abstract: The invention relates to a preparation method of an enzyme detergent for wooden furniture. According to the technical scheme, the enzyme detergent is prepared by mixing L-sodium ascorbate, Trichoderma longibrachiatum, bifidobacterium adolescentis, oat polypeptides, polyquaternary ammonium salt, wheat germ, costus oil, green tea essence, AES, probiotics, methyl ethyl ester, fish collagen, TX10 emulsifier, trehalose, Kathon 2, sodium alcohol ether sulfate, enzymes, acetic acid, lemon flavor and water.

Enzyme heavy oil stain strong-cleaning agent preparation method

Abstract: The technical scheme of the enzyme heavy oil stain strong-cleaning agent of the present invention is that the enzyme heavy oil stain strong-cleaning agent is prepared by mixing a stephania tetrandra extract, dipotassium glycyrrhizinate, a natto fermentation extract, L-ascorbate sodium, Trichoderma longibrachiatum, Bifidobacterium adolescentis, artemisia argyi oil, green tea essence, AES, probiotic flora, methyl ethyl ester, fish collagen, a TX10 emulsifier, trehalose, kathon 2, docusate sodium, enzyme, acetic acid, apple perfume, and water.

Preparation method of enzyme kitchen range cleaning agent

Abstract: An enzyme kitchen range cleaning agent is prepared by mixing L-sodium ascorbate, Trichoderma longibrachiatum, bifidobacterium adolescentis, tea saponin, ceramide, wheat germs, patchouli oil, Green tea flavor, AES, probiotics, methyl ethyl ester, fish collagen protein, citric acid, trehalose, disodium hydrogen phosphate, Dioctyl Sodium Sulfosuccinate, enzyme, acetic acid, apple incense and water.

Preparation method of an enzyme detergent for automotive glass

Abstract: The invention relates to a preparation method of an enzyme detergent for automotive glass. According to the technical scheme, the enzyme detergent is prepared by mixing Stephania tetrandra root extract, dipotassium glycyrrhizinate, natto fermentation extract, oat polypeptides, polyquaternary ammonium salt, bisabolol, cortex magnoliae officinalis oil, green tea essence, AES, L-sodium ascorbate, Trichoderma longibrachiatum, bifidobacterium adolescentis, ethanol, trehalose, Kathon 2, sodium alcohol ether sulfate, enzymes, acetic acid, lemon flavor and water.

Preparation method of enzyme detergent for glass

Abstract: The invention discloses a preparation method of an enzyme detergent for glass. According to the technical scheme, the enzyme detergent for glass is prepared by mixing extract of stephania tetrandra root, natto fermentation extract, oat polypeptide, polyquaternium, wheat germ, chuanxiong rhizome oil, green tea essence, AES, probiotics, methyl ethyl ester, fish collagen protein, emulsifier TX10, trehalose, Kathon 2, L-sodium ascorbate, trichoderma longibrachiatum, bifidobacterium adolescentis, apple flavor and water.