scispace - formally typeset
Search or ask a question
Topic

Trichoderma longibrachiatum

About: Trichoderma longibrachiatum is a research topic. Over the lifetime, 452 publications have been published within this topic receiving 10591 citations.


Papers
More filters
Journal ArticleDOI
TL;DR: This is the first report on ion‐channel forming peptaibols with synergistic toxicity from T. longibrachiatum strains isolated from clinical samples, and the toxic effects on boar sperm cells depended on these peptibols, named trilongins.
Abstract: Certain species of the filamentous fungal genus Trichoderma (e.g. Trichoderma longibrachiatum and Trichoderma citrinoviride) are among the emerging clinical pathogens and also the most common species in the indoor space of mould-damaged buildings. The molecules involved in its pathology are not known. In the present study, we report that 0.5-2.6 wt% of the T. longibrachiatum mycelial biomass consisted of thermostable secondary metabolites mitochondriotoxic to mammalian cells. These were identified by LC/MS as one 11-residue and eight 20-residue peptaibols, AcAib-Asn-Leu/Ile-Leu/Ile-Aib-Pro-Leu/Ile-Leu/Ile-Aib-Pro-Leuol/Ileol (1175 Da) and AcAib-Ala-Aib-Ala-Aib-Ala/Aib-Gln-Aib-Val/Iva-Aib-Gly-Leu/Ile-Aib-Pro-Val/Iva-Aib-Val/Iva/Aib-Gln/Glu-Gln-Pheol(1936-1965 Da) (Aib, α-aminoisobutyric acid; Ac, acetyl; Ileol, isoleucinol; Iva, isovaline; Leuol, leucinol; Pheol, phenylalaninol). The toxic effects on boar sperm cells depended on these peptaibols, named trilongins. The trilongins formed voltage dependent, Na(+)/K(+) permeable channels in biomembranes. The permeability ratios for Na(+) ions, relative to K(+), of the 11-residue trilongin channel (0.95 : 1) and the 20-residue trilongin channel (0.8 : 1) were higher than those of alamethicin. The combined 11-residue and 20-residue trilongins generated channels that remained in an open state for a longer time than those formed by either one of the peptaibols alone. Corresponding synergy was observed in toxicokinetics. With 11-residue and 20-residue trilongins combined 1 : 2 w/w, an effective median concentration (EC(50) ) of 0.6 μg·mL(-1) was reached within 30 min, and the EC(50) shifted down to 0.2 μg·mL(-1) upon extended exposure. By contrast, with 11-residue or 20-residue trilonging separately in 30 min of exposure, the EC(50) values were 15 and 3 μg·mL(-1) , respectively, and shifted down to 1.5 and 0.4 μg·mL(-1) upon extended exposure. This is the first report on ion-channel forming peptaibols with synergistic toxicity from T. longibrachiatum strains isolated from clinical samples.

63 citations

Journal ArticleDOI
TL;DR: A case of acute invasive sinusitis due to an unusual moniliaceous fungus, Trichoderma longibrachiatum Rifai 1969, in a small bowel and liver transplant recipient treated with tacrolimus (FK-506) and prednisone is described.
Abstract: We describe a case of acute invasive sinusitis due to an unusual moniliaceous fungus, Trichoderma longibrachiatum Rifai 1969 (a member of the class Hyphomycetes), in a small bowel and liver transplant recipient treated with tacrolimus (FK-506) and prednisone The patient was successfully treated with surgical debridements and amphotericin B followed by oral itraconazole

62 citations

Journal ArticleDOI
TL;DR: The role, and functions of some major biocontrol genes present in the Trichoderma species viz., Trichodingma harzianum, Trichodma viride, TrICHoderma atroviride , Trichodyma reesei, Trchoderma hamatum and Trichodema longibrachiatum are explained.
Abstract: Accepted 25 November, 2011 In the world, the traditional agricultural practices are getting affected by various problems such as disease, pest, drought, decreased soil fertility due to use of hazardous chemical pesticides, pollution and global warming. As a result, there is a need for some eco-friendly biocontrol agents that help in resolving the previous mentioned problems. The various types of biological control agents such as bacteria and fungi are involved in biocontrol activity. Among them, fungal genus Trichoderma plays a major role in controlling the plant diseases. The species of Trichoderma are known to produce different kinds of enzymes which have a significant role in biocontrol activity like cell wall degradation, biotic and abiotic stress tolerance, hyphal growth, antagonistic activity against plant pathogens. By the advance techniques laid in the molecular biology, we can easily isolate, characterize, clone, sequence and express the functions of these genes and can study their functions and role in the biocontrol mechanism. This review article explains about the role, and functions of some major biocontrol genes present in the Trichoderma species viz., Trichoderma harzianum , Trichoderma viride , Trichoderma atroviride , Trichoderma reesei , Trichoderma hamatum and Trichoderma longibrachiatum .

61 citations

Patent
17 Mar 1993
TL;DR: In this paper, the EG III cellulase enzyme from Trichoderma longibrachiatum and the amino acid sequence of the secreted (mature) and non-secreted (preprotein) forms of the enzyme were presented.
Abstract: The present invention is directed to purified EG III cellulase enzyme isolated from Trichoderma longibrachiatum and the amino acid sequence of the secreted (mature) and non-secreted (preprotein) forms. The present invention is further directed to the DNA fragment and sequence that encodes the EG III cellulase enzyme. Also disclosed are methods for isolating either purified or highly enriched EG III cellulase obtained from Trichoderma spp. or genetically modified strains of Trichoderma spp.

61 citations

Journal ArticleDOI
TL;DR: A novel S-adenosyl-l-methionine (SAM)-dependent methyltransferase catalyzing the O methylation of several chlorophenols and other halogenated phenols was purified 220-fold to apparent homogeneity from mycelia of Trichoderma longibrachiatum CECT 20431.
Abstract: A novel S-adenosyl-l-methionine (SAM)-dependent methyltransferase catalyzing the O methylation of several chlorophenols and other halogenated phenols was purified 220-fold to apparent homogeneity from mycelia of Trichoderma longibrachiatum CECT 20431. The enzyme could be identified in partially purified protein preparations by direct photolabeling with [methyl-3H]SAM, and this reaction was prevented by previous incubation with S-adenosylhomocysteine. Gel filtration indicated that the Mr was 112,000, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the enzyme was composed of two subunits with molecular weights of approximately 52,500. The enzyme had a pH optimum between 8.2 and 8.5 and an optimum temperature of 28°C, with a pI of 4.9. The Km values for 2,4,6-trichlorophenol and SAM were 135.9 ± 12.8 and 284.1 ± 35.1 μM, respectively. S-Adenosylhomocysteine acted as a competitive inhibitor, with a Ki of 378.9 ± 45.4 μM. The methyltransferase was also strongly inhibited by low concentrations of several metal ions, such as Cu2+, Hg2+, Zn2+, and Ag+, and to a lesser extent by p-chloromercuribenzoic acid, but it was not significantly affected by several thiols or other thiol reagents. The methyltransferase was specifically induced by several chlorophenols, especially if they contained three or more chlorine atoms in their structures. Substrate specificity studies showed that the activity was also specific for halogenated phenols containing fluoro, chloro, or bromo substituents, whereas other hydroxylated compounds, such as hydroxylated benzoic acids, hydroxybenzaldehydes, phenol, 2-metoxyphenol, and dihydroxybenzene, were not methylated.

59 citations


Network Information
Related Topics (5)
Bacillus subtilis
19.6K papers, 539.4K citations
76% related
Chitin
6.5K papers, 253.9K citations
76% related
Bacteria
23.6K papers, 715.9K citations
74% related
Cellulase
16.1K papers, 479.5K citations
74% related
Yeast
31.7K papers, 868.9K citations
74% related
Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20221
202121
202026
201926
201819
201723