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Trichoderma longibrachiatum
About: Trichoderma longibrachiatum is a research topic. Over the lifetime, 452 publications have been published within this topic receiving 10591 citations.
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TL;DR: The role of competition for nitrogen in interactions of pathogenic fungi and antagonists was investigated and the antagonistic fungi used nitrogen as ammonium at statistically significant higher rates during the first six days of cultivation than the phytopathogenic fungi.
57 citations
TL;DR: A marine strain of Trichoderma longibrachiatum isolated from blue mussels (Mytilus edulis) was investigated for short peptaibol production and there was an exponential relation between their relative hydrophobicity and their cytotoxicity on KB cells.
56 citations
TL;DR: A laminarinase has been purified from Trichoderma longibrachiatum cultivated with d-glucose as the growth substrate and was found to hydrolyze laminationarin to oligosaccharides varying in size from glucose to pentaose and to lesser amounts of larger oligosACcharides.
Abstract: A laminarinase [endo-(1,3)-β-d-glucanase] has been purified from Trichoderma longibrachiatum cultivated with d-glucose as the growth substrate. The enzyme was found to hydrolyze laminarin to oligosaccharides varying in size from glucose to pentaose and to lesser amounts of larger oligosaccharides. The enzyme was unable to cleave laminaribiose but hydrolyzed triose to laminaribiose and glucose. The enzyme cleaved laminaritetraose, yielding laminaritriose, laminaribiose, and glucose, and similarly cleaved laminaripentaose, yielding laminaritetraose, laminaritriose, laminaribiose, and glucose. The enzyme cleaved only glucans containing β-1,3 linkages. The pH and temperature optima were 4.8 and 55°C, respectively. Stability in the absence of a substrate was observed at temperatures up to 50°C and at pH values between 4.9 and 9.3. The molecular mass was determined to be 70 kilodaltons by sodium dodecyl sulfate-12.5% polyacrylamide gel electrophoresis, and the pI was 7.2. Enzyme activity was significantly inhibited in the presence of HgCl2, MnCl2, KMnO4, and N-bromosuccinimide. The Km of the enzyme on laminarin was 0.0016%, and the Vmax on laminarin was 3,170 μmol of glucose equivalents per mg of the pure enzyme per min.
55 citations
TL;DR: Among these isolates, D-3-1 (T. longibrachiatum ) showed the strongest inhibition of the growth of Fusarium sambucinum .
Abstract: One hundred and forty six (146) isolates of Trichoderma spp. were obtained from rhizosphere soils of potato plants in the middle areas of Gansu Province, China. By means of dual culture method, they were examined for antagonism against Fusarium sambucinum , which causes potato dry rot. Ten of the isolates were found to be evidently antagonistic to the pathogen. Based on morphological characteristics and molecular analyses, the antagonistic isolates were identified as Trichoderma harzianum Rifai , Trichoderma longibrachiatum Rifai, Trichoderma atroviride Karsten and Trichoderma virens. Among these isolates, D-3-1 (T. longibrachiatum ) showed the strongest inhibition of the growth of Fusarium sambucinum . Key words: Trichoderma , potato, dry rot, biological control, Fusarium sambucinum.
55 citations
TL;DR: The methodology used to establish the sequences of short peptaibols in a mixture without previous individual separation is described and eight of them are new, namely as trichobrachin A I-IV (Aib9-Pro10 sequence) and as Trichoderma longibrachin B I- IV (Val9- pro10 sequence).
Abstract: The production of peptaibols by a marine-related Trichoderma longibrachiatum strain was studied using electrospray ionisation multiple-stage ion trap mass spectrometry (ESI-MSn-IT) and gas chromatography/electron impact mass spectrometry (GC/EI-MS). Two major groups of peptaibols were identified, those with long sequences (20 amino acids) and others with short sequences (11 amino acids). This paper describes the methodology used to establish the sequences of short peptaibols in a mixture without previous individual separation. Nine peptaibols were identified. Among them, eight are new, namely as trichobrachin A I-IV (Aib9-Pro10 sequence) and as trichobrachin B I-IV (Val9-Pro10 sequence). Original Pro6-Val7 and Val9-Pro10 sequences have to be noted.
54 citations