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Trichoderma longibrachiatum

About: Trichoderma longibrachiatum is a research topic. Over the lifetime, 452 publications have been published within this topic receiving 10591 citations.


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Journal ArticleDOI
TL;DR: ABFI and BXLI correspond to a previously purified alpha-L-arabinofuranosidase and a beta-xylosidase from T. reesei, respectively, as confirmed by partial amino acid sequencing of the Trichoderma-produced enzymes.
Abstract: A cDNA expression library of Trichoderma reesei RutC-30 was constructed in the yeast Saccharomyces cerevisiae Two genes, abf1 and bxl1, were isolated by screening the yeast library for extracellular alpha-L-arabinofuranosidase activity with the substrate p-nitrophenyl-alpha-L-arabinofuranoside The genes abf1 and bxl1 encode 500 and 758 amino acids, respectively, including the signal sequences The deduced amino acid sequence of ABFI displays high-level similarity to the alpha-L-arabinofuranosidase B of Aspergillus niger, and the two can form a new family of glycosyl hydrolases The deduced amino acid sequence of BXLI shows similarities to the beta-glucosidases grouped in family 3 The yeast-produced enzymes were tested for enzymatic activities against different substrates ABFI released L-arabinose from p-nitrophenyl-alpha-L-arabinofuranoside and arabinoxylans and showed some beta-xylosidase activity toward p-nitrophenyl-beta-D-xylopyranoside BXLI did not release L-arabinose from arabinoxylan It showed alpha-L-arabinofuranosidase, alpha-L-arabinopyranosidase, and beta-xylosidase activities against p-nitrophenyl-alpha-L-arabinofuranosidase, p-nitrophenyl-alpha-L-arabinopyranoside, and p-nitrophenyl-beta-D- xylopyranoside, respectively, with the last activity being the highest It was also able to hydrolyze xylobiose and slowly release xylose from polymeric xylan ABFI and BXLI correspond to a previously purified alpha-L-arabinofuranosidase and a beta-xylosidase from T reesei, respectively, as confirmed by partial amino acid sequencing of the Trichoderma-produced enzymes Both enzymes produced in yeasts displayed hydrolytic properties similar to those of the corresponding enzymes purified from T reesei

188 citations

Journal ArticleDOI
TL;DR: The synergistic effect between ruminal and exogenous enzymes increases the hydrolytic potential within the rumen environment and is likely a significant mechanism by which enzyme additives improve feed digestion in ruminants.

180 citations

Journal ArticleDOI
TL;DR: The results indicated that the strain of T6 ameliorated the adverse effects significantly, protecting the seedlings from salt stress during their growth period, and may be due to the improvement of the antioxidative defense system and gene expression in the stressed wheat plants.
Abstract: Soil salinity is a serious problem worldwide that reduces agricultural productivity. Trichoderma longibrachiatum T6 (T6) has been shown to promote wheat growth and induce plant resistance to parasitic nematodes, but whether the plant-growth-promoting fungi T6 can enhance plant tolerance to salt stress is unknown. Here we determined the effect of plant-growth-promoting fungi T6 on wheat seedlings’ growth and development under salt stress, and investigated the role of T6 in inducing the resistance to NaCl stress at physiological, biochemical, and molecular levels. Wheat seedlings were inoculated with the strain of T6 and then compared with non-inoculated controls. Shoot height, root length, and shoot and root weights were measured on 15 days old wheat seedlings grown either under 150 mM NaCl or in a controlled setting without any NaCl. A number of colonies were re-isolated from the roots of wheat seedlings under salt stress. The relative water content in the leaves and roots, chlorophyll content, and root activity were significantly increased, and the accumulation of proline content in leaves was markedly accelerated with the plant growth parameters, but the content of leaf malondialdehyde (MDA) under saline condition was significantly decreased. The antioxidant enzymes-superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) in wheat seedlings were increased by 29%, 39% and 19%, respectively, with the application of the strain of T6 under salt stress; the relative expression of SOD, POD and CAT genes in these wheat seedlings were significantly up-regulated. Our results indicated that the strain of T6 ameliorated the adverse effects significantly, protecting the seedlings from salt stress during their growth period. The possible mechanisms by which T6 suppresses the negative effect of NaCl stress on wheat seedling growth may be due to the improvement of the antioxidative defense system and gene expression in the stressed wheat plants.

179 citations

Journal ArticleDOI
TL;DR: This review introduces to the scientific community the development of modern tools for Trichoderma species identification: the oligonucleotide barcode program TrichOKEY version 1.0, and TrichoBLAST, the multilocus database of vouchered sequences powered by a similarity search tool, which make it possible to identify all known Trichodma species based on sequence analysis.

176 citations

Journal ArticleDOI
TL;DR: Trichogin A IV (GA IV) is the main component of the natural trichog in mixture, a new peptide group extracted from in vitro cultures of the fungus Trichoderma longibrachiatum, and its amino acid sequence was elucidated by FAB mass spectrometry and high-field NMR.
Abstract: Trichogin A IV (GA IV) is the main component of the natural trichogin mixture, a new peptide group extracted from in vitro cultures of the fungus Trichoderma longibrachiatum. GA IV was isolated by reversed-phase HPLC, and its amino acid sequence was elucidated by FAB mass spectrometry and high-field NMR. Complete 1 H and 13 C resonance assignments were carried out using HOHAHA, ROESY, 1 H- 13 C COSY, and COLOC two-dimensional spectroscopies

176 citations


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Performance
Metrics
No. of papers in the topic in previous years
YearPapers
20221
202121
202026
201926
201819
201723