Topic
Trichoderma longibrachiatum
About: Trichoderma longibrachiatum is a research topic. Over the lifetime, 452 publications have been published within this topic receiving 10591 citations.
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TL;DR: A comparatively high diversity of species was found, comprising 29 taxa, including T. asperellum was the prevalent species and was represented by two distinct genotypes with different metabolic profiles and habitat preferences, along with 11 currently undescribed species.
168 citations
TL;DR: All xylans generated XO with different degree of polymerization (DP), but the DP of XO depended on the enzyme specificity and the structure of substrate, according to the structural differences of the xylan types.
147 citations
TL;DR: The data suggest that the SA pathway and ethylene pathway together coordinate the activation of defense mechanisms in cellulase-induced cotyledons and a partial interaction between both signaling pathways is found.
Abstract: Infiltration of cellulase (EC 3.2.1.4) from Trichoderma longibrachiatum into melon (Cucumis melo) cotyledons induced several key defense mechanisms and hypersensitive reaction-like symptoms. An oxidative burst was observed 3 hours after treatment and was followed by activation of ethylene and salicylic acid (SA) signaling pathways leading to marked induction of peroxidase and chitinase activities. The treatment of cotyledons by heat-denatured cellulase also led to some induction of peroxidase and chitinase activities, but the oxidative burst and SA production were not observed. Co-infiltration of aminoethoxyvinil-glycine (an ethylene inhibitor) with the active cellulase did not affect the high increase of peroxidase and chitinase activities. In contrast, co-infiltration of aminoethoxyvinil-glycine with the denatured enzyme blocked peroxidase and chitinase activities. Our data suggest that the SA pathway (induced by the cellulase activity) and ethylene pathway (induced by heat-denatured and active protein) together coordinate the activation of defense mechanisms. We found a partial interaction between both signaling pathways since SA caused an inhibition of the ethylene production and a decrease in peroxidase activity when co-infiltrated with denatured cellulase. Treatments with active or denatured cellulase caused a reduction in powdery mildew (Sphaerotheca fuliginea) disease.
145 citations
Patent•
24 May 1995TL;DR: In this paper, the cloning and high level expression of novel truncated cellulase proteins or derivatives thereof in the filamentous fungus Trichoderma longibrachiatum were discussed.
Abstract: The present invention relates to the cloning and high level expression of novel truncated cellulase proteins or derivatives thereof in the filamentous fungus Trichoderma longibrachiatum. Further aspects of the present invention relate to fungal transformants that express the novel truncated cellulases and derivatives, and expression vectors comprising the DNA gene fragments or variants thereof that code for the truncated cellulases derived from Trichoderma longibrachiatum using genetic engineering techniques.
141 citations
TL;DR: Mixed culturing is beneficial for the economic production of cellulases on nutritionally poor agricultural residues, without the need for supplementation with expensive organic supplements.
138 citations