Trichoderma longibrachiatum

About: Trichoderma longibrachiatum is a research topic. Over the lifetime, 452 publications have been published within this topic receiving 10591 citations.

Production and characterization of cellulolytic activities produced by Trichoderma longibrachiatum (GHL)

Abstract: The indigenous cellulolytic fungus Trichoderma longibrachiatum (GHL) isolated from soil near an Algerian hot spring was used for the production of cellulases by submerged fermentation on Mandels medium with cellulose Avicel (1%) as the sole carbon source. Endoglucanase and filter paper activities of the wild-type strain of Trichoderma were compared to the hypercellulolytic mutated Trichoderma reesei Rut C-30, in shake flask cultures at 35°C. After seven days of fermentation, T. longibrachiatum show equivalent activities than T. reesei (10.61 IU/ml of endoglucanase (CMCase) and 2.04 IU/ml of filter paper activity (FPA)). On the other hand, the s-glucosidase activity of Trichoderma GHL was twice more important than that of T. reseei . The influence of inoculum size on cellulase activities did not prove significant differences in enzymatic activities for spore concentrations between 105 and 108 spores/ml. The cellulases produced by the isolated strain were also characterized. The optimum temperatures were 55 and 60°C for endoglucanase and FPA, respectively. The endoglucanase was thermostable at 70°C after 5 h incubation, and it preserved 80% of the original activity. The half-life of the FPA appeared to be 3 h at 60°C. The endoglucanase was optimally active at pH 4.0, and the FPA was optimal at pH 4.0 and 5.0. These activities were stable at 50°C after 5 h incubation in a pH range of 3.0 to 6.0 and 4.0 to 6.0, respectively. These results suggest that the non-mutated strain T. longibrachiatum (GHL) should be an attractive producer for cellulases production. Keywords : Cellulase, Trichoderma reesei, Trichoderma longibrachiatum , submerged fermentation, characterization African Journal of Biotechnology Vol. 12(5), pp. 465-475

Inhibition of ruminant feed enzyme polysaccharidase activities by extracts from silages

Abstract: Extracts from 14 barley silages inhibited endo-1, 4-β-xylanase and α-amylase activities of a ruminant feed enzyme additive from Trichoderma longibrachiatum by 23 to 50% but had little effect on cellulase activity. The inhibitory factor(s) were <10 kDa in size and were stable to autoclaving. These observations may explain why feed enzymes are generally less effective when applied to silages than when applied to dry feeds. Key words: Silage, fibrolytic enzymes, Trichoderma, xylanase, inhibitors

Purification and molecular cloning of eg iii cellulase

Abstract: The present inventon is directed to purified EG III cellulase enzyme isolated from Trichoderma longibrachiatum and the amino acid sequence of the secreted (mature) and non-secreted (preprotein) forms. The present invention is further directed to the DNA fragment and sequence that encodes the EG III cellulase enzyme. Also disclosed are methods for isolating either purified or highly enriched EG III cellulase obtained from Trichoderma spp. or genetically modified strains of $i(Trichoderma spp.)